Imperial College London
Alternate

DrGeraldLarrouy-Maumus

Faculty of Natural Sciences, Department of Life Sciences

Reader in Molecular Microbiology
 
 
 
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Contact

 

+44 (0)20 7594 7463g.larrouy-maumus

 
 
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Location

 

3.42Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Dortet:2020:jac/dkz405,
author = {Dortet, L and Broda, A and bernabeu, S and GLUPCZYNSKI, Y and bogaerts, P and bonnin, R and naas, T and Filloux, A and Larrouy-Maumus, G},
doi = {jac/dkz405},
journal = {Journal of Antimicrobial Chemotherapy},
pages = {110--116},
title = {Optimization of the MALDIxin test for the rapid identification of colistin resistance in Klebsiella pneumoniae using MALDI-TOF-MS},
url = {http://dx.doi.org/10.1093/jac/dkz405},
volume = {75},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Background. With the dissemination of carbapenemase producers, a revival of colistin was observed for the treatment of infections caused by multidrug-resistant Gram-negatives. Unfortunately, the increasing usage of colistin led to the emergence of resistance. In Klebsiella pneumoniae, colistin resistance arises through addition of L-arabinose-4N (L-Ara4N) or phosphoethanolamine (pEtN) on the native lipid A. The underlying mechanisms involve numerous chromosome-encoded genes or the plasmid-encoded phosphoethanolamine transferase MCR. Currently, detection of colistin resistance is time consuming since it still relies on MIC determination by broth microdilution. Recently, a rapid diagnostic test based on MALDI-TOF detection of modified lipid A was developed (the MALDIxin test) and tested on Escherichia coli and Acinetobacter baumannii.Objectives. Optimize the MALDIxin test for the rapid detection of colistin resistance in Klebsiella pneumoniae.Methods. This optimization consists on an additional mild-acid hydrolysis of 15 min in 1% acetic acid. The optimized method was tested on a collection of 81 clinical K. pneumoniae isolates including 49 colistin resistant strains among which 45 correspond to chromosome-encoded resistance, 3 MCR-related resistance and one isolate harbouring both mechanisms.Results. The optimized method allowed the rapid (< 30 min) identification of L-Ara4N and pEtN modified lipid A of K. pneumoniae which are known to be the real triggers of polymyxin resistance. In the same time, it discriminates between chromosome-encoded and MCR-related polymyxin resistance.Conclusions. The MALDIxin test has the potential to become an accurate tool for the rapid diagnostic of colistin resistance in clinically-relevant Gram negative bacteria.
AU  - Dortet,L
AU  - Broda,A
AU  - bernabeu,S
AU  - GLUPCZYNSKI,Y
AU  - bogaerts,P
AU  - bonnin,R
AU  - naas,T
AU  - Filloux,A
AU  - Larrouy-Maumus,G
DO  - jac/dkz405
EP  - 116
PY  - 2020///
SN  - 0305-7453
SP  - 110
TI  - Optimization of the MALDIxin test for the rapid identification of colistin resistance in Klebsiella pneumoniae using MALDI-TOF-MS
T2  - Journal of Antimicrobial Chemotherapy
UR  - http://dx.doi.org/10.1093/jac/dkz405
UR  - https://academic.oup.com/jac/article/75/1/110/5580627
UR  - http://hdl.handle.net/10044/1/73328
VL  - 75
ER  -
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