Imperial College London
Alternate

DrGeraldLarrouy-Maumus

Faculty of Natural Sciences, Department of Life Sciences

Reader in Molecular Microbiology
 
 
 
//

Contact

 

+44 (0)20 7594 7463g.larrouy-maumus

 
 
//

Location

 

3.42Flowers buildingSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Wan:2021:10.1101/2021.10.28.21265436,
author = {Wan, Y and Myall, AC and Boonyasiri, A and Bolt, F and Ledda, A and Mookerjee, S and Weiße, AY and Getino, M and Turton, JF and Abbas, H and Prakapaite, R and Sabnis, A and Abdolrasoulia, A and Malpartida-Cardenas, K and Miglietta, L and Donaldson, H and Gilchrist, M and Hopkins, KL and Ellington, MJ and Otter, JA and Larrouy-Maumus, G and Edwards, AM and Rodriguez-Manzano, J and Didelot, X and Barahona, M and Holmes, AH and Jauneikaite, E and Davies, F},
doi = {10.1101/2021.10.28.21265436},
title = {Integrated analysis of patient networks and plasmid genomes reveals a regional, multi-species outbreak of carbapenemase-producing Enterobacterales carrying both<i>bla</i><sub>IMP</sub>and<i>mcr-9</i>genes},
url = {http://dx.doi.org/10.1101/2021.10.28.21265436},
year = {2021}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - <jats:title>Abstract</jats:title><jats:sec><jats:title>Background</jats:title><jats:p>Carbapenemase-producing Enterobacterales (CPE) are challenging in the healthcare setting, with resistance to multiple classes of antibiotics and a high associated mortality. The incidence of CPE is rising globally, despite enhanced awareness and control efforts. This study describes an investigation of the emergence of IMP-encoding CPE amongst diverse Enterobacterales species between 2016 and 2019 in patients across a London regional hospital network.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>We carried out a network analysis of patient pathways, using electronic health records, to identify contacts between IMP-encoding CPE positive patients. Genomes of IMP-encoding CPE isolates were analysed and overlayed with patient contacts to imply potential transmission events.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>Genomic analysis of 84 Enterobacterales isolates revealed diverse species (predominantly<jats:italic>Klebsiella</jats:italic>spp,<jats:italic>Enterobacter</jats:italic>spp,<jats:italic>E. coli</jats:italic>), of which 86% (72/84) harboured an IncHI2 plasmid, which carried both<jats:italic>bla</jats:italic><jats:sub>IMP</jats:sub>and the mobile colistin resistance gene<jats:italic>mcr-9</jats:italic>(68/72). Phylogenetic analysis of IncHI2 plasmids identified three lineages which showed significant association with patient contact and movements between four hospital sites and across medical specialities, which had been missed on initial investigations.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>Combined, our patient network and plasmid analyses demonstrate an interspecies, plasmid-med
AU  - Wan,Y
AU  - Myall,AC
AU  - Boonyasiri,A
AU  - Bolt,F
AU  - Ledda,A
AU  - Mookerjee,S
AU  - Weiße,AY
AU  - Getino,M
AU  - Turton,JF
AU  - Abbas,H
AU  - Prakapaite,R
AU  - Sabnis,A
AU  - Abdolrasoulia,A
AU  - Malpartida-Cardenas,K
AU  - Miglietta,L
AU  - Donaldson,H
AU  - Gilchrist,M
AU  - Hopkins,KL
AU  - Ellington,MJ
AU  - Otter,JA
AU  - Larrouy-Maumus,G
AU  - Edwards,AM
AU  - Rodriguez-Manzano,J
AU  - Didelot,X
AU  - Barahona,M
AU  - Holmes,AH
AU  - Jauneikaite,E
AU  - Davies,F
DO  - 10.1101/2021.10.28.21265436
PY  - 2021///
TI  - Integrated analysis of patient networks and plasmid genomes reveals a regional, multi-species outbreak of carbapenemase-producing Enterobacterales carrying both<i>bla</i><sub>IMP</sub>and<i>mcr-9</i>genes
UR  - http://dx.doi.org/10.1101/2021.10.28.21265436
ER  -
Download