Imperial College London

ProfessorGuyRutter

Faculty of MedicineDepartment of Medicine

Chair in Cell Biology and Head of Cell Biology
 
 
 
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Contact

 

+44 (0)20 7594 3340g.rutter Website

 
 
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Assistant

 

Ms Shazi Singh +44 (0)20 7594 3391

 
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Location

 

327ICTEM buildingHammersmith Campus

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Summary

 

Publications

Publication Type
Year
to

484 results found

Buenaventura T, Kanda N, Douzenis PC, Jones B, Bloom SR, Chabosseau P, Correa IR, Bosco D, Piemonti L, Marchetti P, Johnson PR, Shapiro AMJ, Rutter GA, Tomas Aet al., 2018, A Targeted RNAi Screen Identifies Endocytic Trafficking Factors That Control GLP-1 Receptor Signaling in Pancreatic beta-Cells, DIABETES, Vol: 67, Pages: 385-399, ISSN: 0012-1797

JOURNAL ARTICLE

Callingham RM, Pullen TJ, Rutter GA, 2018, The role of a long non-coding RNA at the Pax6 locus in controlling beta cell identity and function, Publisher: WILEY, Pages: 50-50, ISSN: 0742-3071

CONFERENCE PAPER

Carrat GR, Haythorne E, Chabosseau P, Hodson D, Pullen TJ, Catala AT, Haataja L, Leclerc I, Arvan P, Rutter GAet al., 2018, The Type 2 diabetes genome-wide association study (GWAS) gene STARD10 controls beta cell granule morphogenesis and proinsulin release, Publisher: WILEY, Pages: 46-46, ISSN: 0742-3071

CONFERENCE PAPER

Chabosseau P, Cheung R, Rutter GA, 2018, CRISPR/Cas9-mediated engineering of insulin-secreting cells to study Type 2 diabetes risk variants of the gene SLC30A8/ZnT8 the Zn2+transporter, Publisher: WILEY, Pages: 45-45, ISSN: 0742-3071

CONFERENCE PAPER

Chabosseau P, Woodier J, Cheung R, Rutter GAet al., 2018, Sensors for measuring subcellular zinc pools, METALLOMICS, Vol: 10, Pages: 229-239, ISSN: 1756-5901

JOURNAL ARTICLE

Cheung R, Rolando D, Chabosseau P, Sakamoto K, Smith DM, Rutter GA, Martinez-Sanchez Aet al., 2018, miR-125b is a new regulator of pancreatic beta cell function, Publisher: WILEY, Pages: 47-47, ISSN: 0742-3071

CONFERENCE PAPER

Fine NHF, Doig CL, Elhassan YS, Vierra NC, Marchetti P, Bugliani M, Nano R, Piemonti L, Rutter GA, Jacobson DA, Lavery GG, Hodson DJet al., 2018, Glucocorticoids Reprogram beta-Cell Signaling to Preserve Insulin Secretion, DIABETES, Vol: 67, Pages: 278-290, ISSN: 0012-1797

JOURNAL ARTICLE

Gharavy NM, Li X, Leclerc I, Martinez-Sanchez A, Rutter GAet al., 2018, Roles for the Type 2 diabetes-associated genes C2CD4A and C2CD4B in the control of insulin secretion, Publisher: WILEY, Pages: 40-41, ISSN: 0742-3071

CONFERENCE PAPER

Hamilton A, Zhang Q, Salehi A, Willems M, Knudsen JG, Ringgaard AK, Chapman CE, Gonzalez-Alvarez A, Surdo NC, Zaccolo M, Basco D, Johnson PRV, Ramracheya R, Rutter GA, Galione A, Rorsman P, Tarasov AIet al., 2018, Adrenaline Stimulates Glucagon Secretion by Tpc2-Dependent Ca2+ Mobilization From Acidic Stores in Pancreatic α-Cells., Diabetes, Vol: 67, Pages: 1128-1139

Adrenaline is a powerful stimulus of glucagon secretion. It acts by activation of β-adrenergic receptors, but the downstream mechanisms have only been partially elucidated. Here, we have examined the effects of adrenaline in mouse and human α-cells by a combination of electrophysiology, imaging of Ca2+ and PKA activity, and hormone release measurements. We found that stimulation of glucagon secretion correlated with a PKA- and EPAC2-dependent (inhibited by PKI and ESI-05, respectively) elevation of [Ca2+]i in α-cells, which occurred without stimulation of electrical activity and persisted in the absence of extracellular Ca2+ but was sensitive to ryanodine, bafilomycin, and thapsigargin. Adrenaline also increased [Ca2+]i in α-cells in human islets. Genetic or pharmacological inhibition of the Tpc2 channel (that mediates Ca2+ release from acidic intracellular stores) abolished the stimulatory effect of adrenaline on glucagon secretion and reduced the elevation of [Ca2+]i Furthermore, in Tpc2-deficient islets, ryanodine exerted no additive inhibitory effect. These data suggest that β-adrenergic stimulation of glucagon secretion is controlled by a hierarchy of [Ca2+]i signaling in the α-cell that is initiated by cAMP-induced Tpc2-dependent Ca2+ release from the acidic stores and further amplified by Ca2+-induced Ca2+ release from the sarco/endoplasmic reticulum.

JOURNAL ARTICLE

Janjuha S, Singh SP, Tsakmaki A, Gharavy SNM, Murawala P, Konantz J, Birke S, Hodson DJ, Rutter GA, Bewick GA, Ninov Net al., 2018, Age-related islet inflammation marks the proliferative decline of pancreatic beta-cells in zebrafish, ELIFE, Vol: 7, ISSN: 2050-084X

JOURNAL ARTICLE

Jenkins A, Lengyel I, Rutter GA, Lowe N, Shai I, Tirosh A, Petro T, Khamaisi M, Andrews S, Zmora N, Gross A, Maret W, Lewis EC, Moran Aet al., 2018, Obesity, diabetes and zinc: A workshop promoting knowledge and collaboration between the UK and Israel, november 28-30, 2016 - Israel., J Trace Elem Med Biol, Vol: 49, Pages: 79-85

JOURNAL ARTICLE

Jones B, Bloom SR, Buenaventura T, Tomas A, Rutter GAet al., 2018, Control of insulin secretion by GLP-1, PEPTIDES, Vol: 100, Pages: 75-84, ISSN: 0196-9781

JOURNAL ARTICLE

Jones B, Buenaventura T, Kanda N, Chabosseau P, Owen BM, Scott R, Goldin R, Angkathunyakul N, Correa IR, Bosco D, Johnson PR, Piemonti L, Marchetti P, Shapiro AMJ, Cochran BJ, Hanyaloglu AC, Inoue A, Tan T, Rutter GA, Tomas A, Bloom SRet al., 2018, Targeting GLP-1 receptor trafficking to improve agonist efficacy, NATURE COMMUNICATIONS, Vol: 9, ISSN: 2041-1723

JOURNAL ARTICLE

Khan R, Kanda N, Bloom SR, Rutter GA, Lindsley CW, Nance KD, Niswender KD, Jones B, Tomas Aet al., 2018, An investigation into the role of compound 5d in incretin-dependent pancreatic beta cell function, Publisher: WILEY, Pages: 54-54, ISSN: 0742-3071

CONFERENCE PAPER

Laughlin WE, Buenaventura T, Oqua AI, Kanda N, Walker A, Klymchenko AS, Bloom SR, Rutter GA, Hanyaloglu A, Jones B, Tomas Aet al., 2018, Control of glucagon-like peptide-1 receptor (GLP-1R) palmitoylation, lipid raft partitioning, clustering and signalling by biased agonism, Publisher: WILEY, Pages: 54-55, ISSN: 0742-3071

CONFERENCE PAPER

Ma Y, Ratnasabapathy R, Izzi-Engbeaya C, Nguyen-Tu M-S, Richardson E, Hussain S, De Backer I, Holton C, Norton M, Carrat G, Schwappach B, Rutter GA, Dhillo WS, Gardiner Jet al., 2018, Hypothalamic arcuate nucleus glucokinase regulates insulin secretion and glucose homeostasis., Diabetes Obes Metab

AIMS: Glucokinase (GK) serves as a glucose sensor in several tissues including glucose-sensitive neurons of the arcuate nucleus within the hypothalamus. We have previously demonstrated a role for arcuate GK in the regulation of food and glucose intake. However, its role in the regulation of glucose homeostasis is less clear. We therefore sought to investigate the role of arcuate GK in the regulation of glucose homeostasis. MATERIALS AND METHODS: Recombinant adeno-associated virus expressing either GK or an antisense GK construct was used to alter GK activity specifically in the hypothalamic arcuate nucleus. GK activity in this nucleus was also increased by stereotactic injection of the GK activator, compound A. The effect of altered arcuate nucleus GK activity on glucose homeostasis was subsequently investigated using glucose and insulin tolerance tests. RESULTS: Increased GK activity specifically within the arcuate nucleus increased insulin secretion and improved glucose tolerance in rats during oral glucose tolerance tests. Decreased GK activity in this nucleus reduced insulin secretion and increased glucose levels during the same tests. Insulin sensitivity was not affected in either case. The effect of arcuate nucleus glucokinase was maintained in a model of type 2 diabetes. CONCLUSIONS: These results demonstrate a role for arcuate nucleus GK in systemic glucose homeostasis. This article is protected by copyright. All rights reserved.

JOURNAL ARTICLE

Martinez-Sanchez A, Nguyen-Tu M-S, Cebola I, Yavari A, Marchetti P, Piemonti L, de Koning E, Shapiro AMJ, Johnson P, Sakamoto K, Smith DM, Leclerc I, Ashrafian H, Ferrer J, Rutter GAet al., 2018, MiR-184 expression is regulated by AMPK in pancreatic islets, FASEB JOURNAL, Vol: 32, Pages: 2587-2600, ISSN: 0892-6638

JOURNAL ARTICLE

Martinez-Sanchez A, Nguyen-Tu M-S, Leclerc I, Rutter GAet al., 2018, Manipulation and Measurement of AMPK Activity in Pancreatic Islets., Methods Mol Biol, Vol: 1732, Pages: 413-431

The role of the energy sensor AMPK-activated protein kinase (AMPK) in the insulin-secreting β-cell remains unclear and a subject of intense research. With this chapter, we aim to provide a detailed description of the methods that our group routinely applies to the study of AMPK function in mouse and human pancreatic islets. Thus, we provide detailed protocols to isolate and/or culture mouse and human islets, to modulate and measure AMPK activity in isolated islets, and to evaluate its impact on islet function.

JOURNAL ARTICLE

Martinez-Sanchez A, Nguyen-Tu MS, Cebola I, Yavari A, Marchetti P, Piemonti L, Leclerc I, Sakamoto K, Smith DM, Rutter GAet al., 2018, Adenosine Monophosphate (AMP)-activated protein kinase (AMPK) regulates the expression of miR-184 and other miRNAs important for beta cell function, Publisher: WILEY, Pages: 48-48, ISSN: 0742-3071

CONFERENCE PAPER

Martinez-Sanchez A, Pizza G, Marchetti P, Piemonti L, Johnson P, Sakamoto K, Smith DK, Leclerc I, Rutter GAet al., 2018, Glucose regulates miR-125b expression via AMP-activated protein kinase (AMPK), Publisher: WILEY, Pages: 49-49, ISSN: 0742-3071

CONFERENCE PAPER

Nguyen-Tu MS, Rutter GA, Xavier GD, 2018, Adipose tissue-selective deletion of the Type 2 diabetes Genome-wide association studies (GWAS) gene Tcf7l2 in mice impairs insulin sensitivity and secretion, Publisher: WILEY, Pages: 83-84, ISSN: 0742-3071

CONFERENCE PAPER

Pullen TJ, Groen N, van Oudenaarden A, Hodson DJ, Carlotti F, Rutter GAet al., 2018, Identification of potential hub beta cells using single-cell RNA-Seq, Publisher: WILEY, Pages: 51-51, ISSN: 0742-3071

CONFERENCE PAPER

Rutter GA, Haythorne EA, Georgiadou E, Xavier GDS, Pullen TJ, Rizzuto R, Martinez-Sanchez A, McGinty JA, French PMet al., 2018, Pancreatic beta cell-selective deletion of the mitochondrial calcium uniporter (MCU) impairs glucose-stimulated insulin secretion in vitro but not in vivo, Publisher: WILEY, Pages: 42-42, ISSN: 0742-3071

CONFERENCE PAPER

Rutter GA, Hodson DJ, 2018, Beta cell connectivity in pancreatic islets: a type 2 diabetes target? (vol 72, pg 453, 2015), CELLULAR AND MOLECULAR LIFE SCIENCES, Vol: 75, Pages: 1303-1305, ISSN: 1420-682X

JOURNAL ARTICLE

Scott F, Parks S, Gao T, Awuapura NJ, Ayathamattam J, Valdivia H, Rutter GA, Leclerc Iet al., 2018, The calcium sensor sorcin maintains activating transcription factor 6 (ATF6) transcriptional activity while lowering ER stress, Publisher: WILEY, Pages: 44-44, ISSN: 0742-3071

CONFERENCE PAPER

Solimena M, Schulte AM, Marselli L, Ehehalt F, Richter D, Kleeberg M, Mziaut H, Knoch K-P, Parnis J, Bugliani M, Siddiq A, Joerns A, Burdet F, Liechti R, Suleiman M, Margerie D, Syed F, Distler M, Gruetzmann R, Petretto E, Moreno-Moral A, Wegbrod C, Soenmez A, Pfriem K, Friedrich A, Meinel J, Wollheim CB, Baretton GB, Scharfmann R, Nogoceke E, Bonifacio E, Sturm D, Meyer-Puttlitz B, Boggi U, Saeger H-D, Filipponi F, Lesche M, Meda P, Dahl A, Wigger L, Xenarios I, Falchi M, Thorens B, Weitz J, Bokvist K, Lenzen S, Rutter GA, Froguel P, von Buelow M, Ibberson M, Marchetti Pet al., 2018, Systems biology of the IMIDIA biobank from organ donors and pancreatectomised patients defines a novel transcriptomic signature of islets from individuals with type 2 diabetes, DIABETOLOGIA, Vol: 61, Pages: 641-657, ISSN: 0012-186X

JOURNAL ARTICLE

Suba K, Nguyen-Tu MS, Chabosseau P, Carrat GR, Leclerc I, Gaboriau DCA, Rothery SM, Salem V, Rutter GAet al., 2018, Measuring real-time islet blood vessel responses to hormonal challenges using the platform of transplanted islets in the anterior chamber of the murine eye, Publisher: WILEY, Pages: 49-49, ISSN: 0742-3071

CONFERENCE PAPER

Tuncay E, Bitirim CV, Olgar Y, Durak A, Rutter GA, Turan Bet al., 2018, Zn2+-transporters ZIP7 and ZnT7 play important role in progression of cardiac dysfunction via affecting sarco(endo)plasmic reticulum-mitochondria coupling in hyperglycemic cardiomyocytes., Mitochondrion

Functional contribution of S(E)R-mitochondria coupling to normal cellular processes is crucial and any alteration in S(E)R-mitochondria axis may be responsible for the onset of diseases. Mitochondrial free Zn2+ level in cardiomyocytes ([Zn2+]Mit) is lower comparison to either its cytosolic or S(E)R level under physiological condition. However, there is little information about distribution of Zn2+-transporters on mitochondria and role of Zn2+-dependent mitochondrial-function associated with [Zn2+]Mit. Since we recently have shown how hyperglycemia (HG)-induced changes in ZIP7 and ZnT7 contribute to Zn2+-transport across S(E)R and contribute to S(E)R-stress in the heart, herein, we hypothesized that these transporters can also be localized to mitochondria and affect the S(E)R-mitochondria coupling, and thereby contribute to cellular Zn2+-muffling between S(E)R-mitochondria in HG-cells. Mitochondrial localizations of ZIP7 and ZnT7 were demonstrated using fluorescence technique while they were confirmed in isolated mitochondrial fractions using biochemical analysis. Markedly decreased ZIP7 and increased ZnT7 levels were measured in isolated mitochondrial fractions from either HG- or doxorubicin, DOX (as positive control)-treated cardiomyocytes. Significantly increases in [Zn2+]Mit and ROS production levels and depolarized mitochondrial membrane potential were also measured in HG cells. The expression levels of some key proteins, responsible for proper S(E)R-mitochondria coupling such as Mfn-1, Fis-1, OPA1, BAP31, STIM1 and PML in either HG- or DOX-cells were supported our above hypothesis, strongly. Overall, this study provides an important description about the role of ZIP7 and ZnT7, localized to both mitochondria and S(E)R and contribute to cellular Zn2+-muffling between cellular-compartments in HG or hypertrophic cardiomyocytes via affecting S(E)R-mitochondria coupling. Any alteration in this axis and/or cellular [Zn2+] may provide new insight for prevention/therapy

JOURNAL ARTICLE

Buenaventura T, Kanda N, Jones B, Correa IR, Bloom SR, Rutter GA, Tomas Aet al., 2017, Characterisation of glucagon-like peptide-1 (GLP-1) receptor trafficking and its significance for pancreatic beta cell function, DIABETIC MEDICINE, Vol: 34, Pages: 53-53, ISSN: 0742-3071

JOURNAL ARTICLE

Carrat G, Haythorne E, Chabosseau P, Hodson DJ, Catala AT, Rutter GAet al., 2017, Role of StarD10 in beta cell physiology, 53rd Annual Meeting of the European-Association-for-the-Study-of-Diabetes (EASD), Publisher: SPRINGER, Pages: S190-S191, ISSN: 0012-186X

CONFERENCE PAPER

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