492 results found
Buenaventura T, Kanda N, Douzenis PC, et al., 2018, A Targeted RNAi Screen Identifies Endocytic Trafficking Factors That Control GLP-1 Receptor Signaling in Pancreatic beta-Cells, DIABETES, Vol: 67, Pages: 385-399, ISSN: 0012-1797
Callingham RM, Pullen TJ, Rutter GA, 2018, The role of a long non-coding RNA at the Pax6 locus in controlling beta cell identity and function, Publisher: WILEY, Pages: 50-50, ISSN: 0742-3071
Carrat GR, Haythorne E, Chabosseau P, et al., 2018, The Type 2 diabetes genome-wide association study (GWAS) gene STARD10 controls beta cell granule morphogenesis and proinsulin release, Publisher: WILEY, Pages: 46-46, ISSN: 0742-3071
Chabosseau P, Cheung R, Rutter GA, 2018, CRISPR/Cas9-mediated engineering of insulin-secreting cells to study Type 2 diabetes risk variants of the gene SLC30A8/ZnT8 the Zn2+transporter, Publisher: WILEY, Pages: 45-45, ISSN: 0742-3071
Cheung R, Rolando D, Chabosseau P, et al., 2018, miR-125b is a new regulator of pancreatic beta cell function, Publisher: WILEY, Pages: 47-47, ISSN: 0742-3071
Fine NHF, Doig CL, Elhassan YS, et al., 2018, Glucocorticoids Reprogram beta-Cell Signaling to Preserve Insulin Secretion, DIABETES, Vol: 67, Pages: 278-290, ISSN: 0012-1797
Gharavy NM, Li X, Leclerc I, et al., 2018, Roles for the Type 2 diabetes-associated genes C2CD4A and C2CD4B in the control of insulin secretion, Publisher: WILEY, Pages: 40-41, ISSN: 0742-3071
Hamilton A, Zhang Q, Salehi A, et al., 2018, Adrenaline Stimulates Glucagon Secretion by Tpc2-Dependent Ca2+ Mobilization From Acidic Stores in Pancreatic alpha-Cells, DIABETES, Vol: 67, Pages: 1128-1139, ISSN: 0012-1797
Izzi-Engbeaya C, Comninos AN, Clarke SA, et al., 2018, The effects of kisspeptin on β-cell function, serum metabolites and appetite in humans., Diabetes Obes Metab
AIMS: To investigate the effect of kisspeptin on glucose-stimulated insulin secretion and appetite in humans. MATERIALS AND METHODS: In 15 healthy men (age: 25.2 ± 1.1 years; BMI: 22.3 ± 0.5 kg m-2 ), we compared the effects of 1 nmol kg-1 h-1 kisspeptin versus vehicle administration on glucose-stimulated insulin secretion, metabolites, gut hormones, appetite and food intake. In addition, we assessed the effect of kisspeptin on glucose-stimulated insulin secretion in vitro in human pancreatic islets and a human β-cell line (EndoC-βH1 cells). RESULTS: Kisspeptin administration to healthy men enhanced insulin secretion following an intravenous glucose load, and modulated serum metabolites. In keeping with this, kisspeptin increased glucose-stimulated insulin secretion from human islets and a human pancreatic cell line in vitro. In addition, kisspeptin administration did not alter gut hormones, appetite or food intake in healthy men. CONCLUSIONS: Collectively, these data demonstrate for the first time a beneficial role for kisspeptin in insulin secretion in humans in vivo. This has important implications for our understanding of the links between reproduction and metabolism in humans, as well as for the ongoing translational development of kisspeptin-based therapies for reproductive and potentially metabolic conditions.
Janjuha S, Singh SP, Tsakmaki A, et al., 2018, Age-related islet inflammation marks the proliferative decline of pancreatic beta-cells in zebrafish, ELIFE, Vol: 7, ISSN: 2050-084X
Jenkins A, Lengyel I, Rutter GA, et al., 2018, Obesity, diabetes and zinc: A workshop promoting knowledge and collaboration between the UK and Israel, november 28-30, 2016-Israel, JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY, Vol: 49, Pages: 79-85, ISSN: 0946-672X
Jones B, Buenaventura T, Kanda N, et al., 2018, Targeting GLP-1 receptor trafficking to improve agonist efficacy, NATURE COMMUNICATIONS, Vol: 9, ISSN: 2041-1723
Khan R, Kanda N, Bloom SR, et al., 2018, An investigation into the role of compound 5d in incretin-dependent pancreatic beta cell function, Publisher: WILEY, Pages: 54-54, ISSN: 0742-3071
Kimura T, Obata A, Shimoda M, et al., 2018, Down-regulation of vascular GLP-1 receptor expression in human subjects with obesity, SCIENTIFIC REPORTS, Vol: 8, ISSN: 2045-2322
Kleiner S, Gomez D, Megra B, et al., 2018, Mice harboring the human SLC30A8 R138X loss-of-function mutation have increased insulin secretory capacity, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 115, Pages: E7642-E7649, ISSN: 0027-8424
Laughlin WE, Buenaventura T, Oqua AI, et al., 2018, Control of glucagon-like peptide-1 receptor (GLP-1R) palmitoylation, lipid raft partitioning, clustering and signalling by biased agonism, Publisher: WILEY, Pages: 54-55, ISSN: 0742-3071
Ma Y, Ratnasabapathy R, Izzi-Engbeaya C, et al., 2018, Hypothalamic arcuate nucleus glucokinase regulates insulin secretion and glucose homeostasis, DIABETES OBESITY & METABOLISM, Vol: 20, Pages: 2246-2254, ISSN: 1462-8902
Martinez-Sanchez A, Nguyen-Tu M-S, Cebola I, et al., 2018, MiR-184 expression is regulated by AMPK in pancreatic islets, FASEB JOURNAL, Vol: 32, Pages: 2587-2600, ISSN: 0892-6638
Martinez-Sanchez A, Nguyen-Tu M-S, Leclerc I, et al., 2018, Manipulation and Measurement of AMPK Activity in Pancreatic Islets., Methods Mol Biol, Vol: 1732, Pages: 413-431
The role of the energy sensor AMPK-activated protein kinase (AMPK) in the insulin-secreting β-cell remains unclear and a subject of intense research. With this chapter, we aim to provide a detailed description of the methods that our group routinely applies to the study of AMPK function in mouse and human pancreatic islets. Thus, we provide detailed protocols to isolate and/or culture mouse and human islets, to modulate and measure AMPK activity in isolated islets, and to evaluate its impact on islet function.
Martinez-Sanchez A, Nguyen-Tu MS, Cebola I, et al., 2018, Adenosine Monophosphate (AMP)-activated protein kinase (AMPK) regulates the expression of miR-184 and other miRNAs important for beta cell function, Publisher: WILEY, Pages: 48-48, ISSN: 0742-3071
Martinez-Sanchez A, Pizza G, Marchetti P, et al., 2018, Glucose regulates miR-125b expression via AMP-activated protein kinase (AMPK), Publisher: WILEY, Pages: 49-49, ISSN: 0742-3071
Millership SJ, Xavier GDS, Choudhury AI, et al., 2018, Neuronatin regulates pancreatic beta cell insulin content and secretion, JOURNAL OF CLINICAL INVESTIGATION, Vol: 128, Pages: 3369-3381, ISSN: 0021-9738
Nguyen-Tu M-S, da Silva Xavier G, Leclerc I, et al., 2018, Transcription factor-7-like 2 (TCF7L2) gene acts downstream of the Lkb1/Stk11 kinase to control mTOR signaling, β cell growth, and insulin secretion., J Biol Chem, Vol: 293, Pages: 14178-14189
Variants in the transcription factor-7-like 2 (TCF7L2/TCF4) gene, involved in Wnt signaling, are associated with type 2 diabetes. Loss of Tcf7l2 selectively from the β cell in mice has previously been shown to cause glucose intolerance and to lower β cell mass. Deletion of the tumor suppressor liver kinase B1 (LKB1/STK11) leads to β cell hyperplasia and enhanced glucose-stimulated insulin secretion, providing a convenient genetic model for increased β cell growth and function. The aim of this study was to explore the possibility that Tcf7l2 may be required for the effects of Lkb1 deletion on insulin secretion in the mouse β cell. Mice bearing floxed Lkb1 and/or Tcf7l2 alleles were bred with knockin mice bearing Cre recombinase inserted at the Ins1 locus (Ins1Cre), allowing highly β cell-selective deletion of either or both genes. Oral glucose tolerance was unchanged by the further deletion of a single Tcf7l2 allele in these cells. By contrast, mice lacking both Tcf7l2 alleles on this background showed improved oral glucose tolerance and insulin secretion in vivo and in vitro compared with mice lacking a single Tcf7l2 allele. Biallelic Tcf7l2 deletion also enhanced β cell proliferation, increased β cell mass, and caused changes in polarity as revealed by the "rosette-like" arrangement of β cells. Tcf7l2 deletion also increased signaling by mammalian target of rapamycin (mTOR), augmenting phospho-ribosomal S6 levels. We identified a novel signaling mechanism through which a modifier gene, Tcf7l2, lies on a pathway through which LKB1 acts in the β cell to restrict insulin secretion.
Nguyen-Tu MS, Rutter GA, Xavier GD, 2018, Adipose tissue-selective deletion of the Type 2 diabetes Genome-wide association studies (GWAS) gene Tcf7l2 in mice impairs insulin sensitivity and secretion, Publisher: WILEY, Pages: 83-84, ISSN: 0742-3071
Pullen TJ, Groen N, van Oudenaarden A, et al., 2018, Identification of potential hub beta cells using single-cell RNA-Seq, Publisher: WILEY, Pages: 51-51, ISSN: 0742-3071
Rutter GA, Haythorne EA, Georgiadou E, et al., 2018, Pancreatic beta cell-selective deletion of the mitochondrial calcium uniporter (MCU) impairs glucose-stimulated insulin secretion in vitro but not in vivo, Publisher: WILEY, Pages: 42-42, ISSN: 0742-3071
Rutter GA, Hodson DJ, 2018, Beta cell connectivity in pancreatic islets: a type 2 diabetes target? (vol 72, pg 453, 2015), CELLULAR AND MOLECULAR LIFE SCIENCES, Vol: 75, Pages: 1303-1305, ISSN: 1420-682X
Scott F, Parks S, Gao T, et al., 2018, The calcium sensor sorcin maintains activating transcription factor 6 (ATF6) transcriptional activity while lowering ER stress, Publisher: WILEY, Pages: 44-44, ISSN: 0742-3071
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