Publications
673 results found
Varadi A, Tsuboi T, Johnson-Cadwell LI, et al., 2003, Kinesin I and cytoplasmic dynein orchestrate glucose-stimulated insulin-containing vesicle movements in clonal MIN6 β-cells, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol: 311, Pages: 272-282, ISSN: 0006-291X
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- Citations: 66
Rutter GA, Da Silva Xavier G, 2003, Roles of 5'-AMP-activated protein kinase (AMPK) in mammalian glucose homoeostasis., Biochem J, Vol: 375, Pages: 1-16
Eto K, Yamashita T, Hirose K, et al., 2003, Glucose metabolism and glutamate analog acutely alkalinize pH of insulin secretory vesicles of pancreatic β-cells, American Journal of Physiology - Endocrinology and Metabolism, Vol: 285, ISSN: 0193-1849
We studied acute changes of secretory vesicle pH in pancreatic β-cells with a fluorescent pH indicator, lysosensor green DND-189. Fluorescence was decreased by 0.66 ± 0.10% at 149 ± 16 s with 22.2 mM glucose stimulation, indicating that vesicular pH was alkalinized by ∼0.016 unit. Glucose-responsive pH increase was observed when cytosolic Ca2+ influx was blocked but disappeared when an inhibitor of glycolysis or mitochondrial ATP synthase was present. Glutamate dimethyl ester (GME), a plasma membrane-permeable analog of glutamate, potentiated glucose-stimulated insulin secretion at 5 mM without changing cellular ATP content or cytosolic Ca2+ concentration ([Ca2+]. Application of GME at basal glucose concentration decreased DND-189 fluorescence by 0.83 ± 0.19% at 38 ± 2 s. These results indicated that the acutely alkalinizing effect of glucose on β-cell secretory vesicle pH was dependent on glucose metabolism but independent of modulations of cytosolic [Ca2+]. Moreover, glutamate derived from glucose may be one of the mediators of this alkalinizing effect of glucose, which may have potential relevance to the alteration of secretory function by glutamate.
Mitchell KJ, Rutter GA, 2003, Role of plasma membrane-related Ca<SUP>2+</SUP>-ATPase-1 (PMR1) in islet β-cell Ca<SUP>2+</SUP> homeostasis., 18th Congress of the International-Diabetes-Federation, Publisher: SPRINGER-VERLAG, Pages: A158-A159, ISSN: 0012-186X
Rutter GA, Nicholls L, Tsuboi T, et al., 2003, Cytoplasmic dynein is not the principle molecular motor involved in retrograde movement of insulin-containing vesicles., 18th Congress of the International-Diabetes-Federation, Publisher: SPRINGER-VERLAG, Pages: A163-A164, ISSN: 0012-186X
Tsuboi T, Rutter GA, 2003, Insulin secretion by 'kiss-and-run' exocytosis in clonal pancreatic islet β-cells, BIOCHEMICAL SOCIETY TRANSACTIONS, Vol: 31, Pages: 833-836, ISSN: 0300-5127
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- Citations: 31
Eto K, Yamashita T, Hirose K, et al., 2003, Glucose metabolism and glutamate analog acutely alkalinize pH of insulin secretory vesicles of pancreatic β-cells, AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, Vol: 285, Pages: E262-E271, ISSN: 0193-1849
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- Citations: 31
Tsuboi T, Lippiat JD, Ashcroft FM, et al., 2003, ATP-dependent interaction of the cytosolic domains of Kir6.2 revealed by fluorescence resonance energy transfer, 18th Congress of the International-Diabetes-Federation, Publisher: SPRINGER-VERLAG, Pages: A161-A161, ISSN: 0012-186X
Xavier GDS, Qian Q, Rutter GA, 2003, Role of insulin and insulin-like growth factor-1 receptors in glucose-regulated preproinsulin gene expression probed by RNA interference and single cell promoter analysis., 18th Congress of the International-Diabetes-Federation, Publisher: SPRINGER-VERLAG, Pages: A153-A153, ISSN: 0012-186X
Rutter GA, 2003, Calcium signalling: NAADP comes out of the shadows., Biochem J, Vol: 373, Pages: e3-e4, ISSN: 0264-6021
The molecular mechanisms through which extracellular stimuli mobilize intracellular Ca(2+) stores are still incompletely understood. Recent work suggests that nicotinic acid-adenine dinucleotide phosphate (NAADP) can finally join the ranks of genuine Ca(2+)-mobilizing second messengers.
Rutter GA, 2003, Insulin secretion: Fatty acid signalling via serpentine receptors, CURRENT BIOLOGY, Vol: 13, Pages: R403-R405, ISSN: 0960-9822
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- Citations: 12
da Silva Xavier G, Leclerc I, Varadi A, et al., 2003, Role for AMP-activated protein kinase in glucose-stimulated insulin secretion and preproinsulin gene expression, Biochem J, Vol: 371, Pages: 761-774, ISSN: 0264-6021
Tsuboi T, Rutter GA, 2003, Multiple forms of "kiss-and-run" exocytosis revealed by evanescent wave microscopy, CURRENT BIOLOGY, Vol: 13, Pages: 563-567, ISSN: 0960-9822
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- Citations: 160
Mitchell KJ, Lai FA, Rutter GA, 2003, Ryanodine receptor type I and nicotinic acid adenine dinucleotide phosphate receptors mediate Ca<SUP>2+</SUP> release from insulin-containing vesicles in living pancreatic β-cells (MIN6), JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 278, Pages: 11057-11064, ISSN: 0021-9258
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- Citations: 154
Tsuboi T, da Silva Xavier G, Holz GG, et al., 2003, Glucagon-like peptide-1 mobilizes intracellular Ca2+ and stimulates mitochondrial ATP synthesis in pancreatic MIN6 beta-cells., Biochem J, Vol: 369, Pages: 287-299
Varadi A, Ainscow EK, Allan VJ, et al., 2002, Involvement of conventional kinesin in glucose-stimulated secretory granule movements and exocytosis in clonal pancreatic β-cells, JOURNAL OF CELL SCIENCE, Vol: 115, Pages: 4177-4189, ISSN: 0021-9533
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- Citations: 116
Zhang P, Bennoun M, Gogard C, et al., 2002, Expression of COUP-TFII in metabolic tissues during development, MECHANISMS OF DEVELOPMENT, Vol: 119, Pages: 109-114, ISSN: 0925-4773
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- Citations: 30
Ainscow EK, Mirshamsi S, Tang T, et al., 2002, Dynamic imaging of free cytosolic ATP concentration during fuel sensing by rat hypothalamic neurones:: evidence for ATP-independent control of ATP-sensitive K<SUP>+</SUP> channels, JOURNAL OF PHYSIOLOGY-LONDON, Vol: 544, Pages: 429-445, ISSN: 0022-3751
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- Citations: 158
Pinton P, Tsuboi T, Ainscow EK, et al., 2002, Dynamics of glucose-induced membrane recruitment of protein kinase C βII in living pancreatic islet β-cells, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 277, Pages: 37702-37710, ISSN: 0021-9258
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- Citations: 76
Andreolas C, da Silva Xavier G, Diraison F, et al., 2002, Stimulation of acetyl-CoA carboxylase gene expression by glucose requires insulin release and sterol regulatory element binding protein 1c in pancreatic MIN6 beta-cells, Diabetes, Vol: 51, Pages: 2536-2545, ISSN: 0012-1797
Diraison F, Ferre P, Foufelle F, et al., 2002, Impact of over-expression of sterol response element binding protein-1c (SREBP-1c) on glucose-stimulated insulin secretion from rat islets., 38th EASD Annual Meeting of the European-Association-for-the-Study-of-Diabetes, Publisher: SPRINGER-VERLAG, Pages: A151-A151, ISSN: 0012-186X
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- Citations: 3
Nicholls LI, Varadi A, Ainscow EK, et al., 2002, Role of an intact mitochondrial reticulum in the triggering of insulin release by glucose., 38th EASD Annual Meeting of the European-Association-for-the-Study-of-Diabetes, Publisher: SPRINGER-VERLAG, Pages: A170-A170, ISSN: 0012-186X
Tsuboi T, Pinton P, Rizzuto R, et al., 2002, Role of localized sub-plasma membrane increases in Ca<SUP>2+</SUP> concentration, detected with a SNAP25.: Pericam chimaera, in the membrane translocation of protein kinase CβII, DIABETES, Vol: 51, Pages: A71-A71, ISSN: 0012-1797
Xavier GD, Leclerc I, Rutter GA, 2002, Glucose-regulated gene expression via inhibition of AMP-activated protein kinase and activated insulin secretion in MIN6 pancreatic β cells, DIABETES, Vol: 51, Pages: A2-A2, ISSN: 0012-1797
Bourdeau I, Bard C, Noël B, et al., 2002, Loss of brain volume in endogenous Cushing's syndrome and its reversibility after correction of hypercortisolism, JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, Vol: 87, Pages: 1949-1954, ISSN: 0021-972X
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- Citations: 167
Varadi A, Ainscow EK, Allan VJ, et al., 2002, Molecular mechanisms involved in secretory vesicle recruitment to the plasma membrane in <i>β</i>-cells, Biochemical Society Transactions, Vol: 30, Pages: 328-332, ISSN: 0300-5127
<jats:p>Glucose stimulates the release of insulin in part by activating the recruitment of secretory vesicles to the cell surface. While this movement is known to be microtubule-dependent, the molecular motors involved are undefined. Active kinesin was found to be essential for vesicle translocation in live β-cells, since microinjection of cDNA encoding dominant-negative KHCmut (motor domain of kinesin heavy chain containing a Thr93 → Asn point mutation) blocked vesicular movements. Moreover, expression of KHCmut strongly inhibited the sustained, but not acute, stimulation of secretion by glucose. Thus, vesicles released during the first phase of insulin secretion exist largely within a translocation-independent pool. Kinesin-driven anterograde movement of vesicles is then necessary for the sustained (second phase) of insulin release. Kinesin may, therefore, represent a novel target for increases in intracellular ATP concentrations in response to elevated extracellular glucose and may be involved in the ATP-sensitive K+channel-independent stimulation of secretion by the sugar.</jats:p>
Leclerc I, Viollet B, da Silva Xavier G, et al., 2002, Role of AMP-activated protein kinase in the regulation of gene transcription, Biochemical Society Transactions, Vol: 30, Pages: 307-311, ISSN: 0300-5127
<jats:p>AMP-activated protein kinase (AMPK) is a regulator of cellular metabolism in response to changes in the energy status of the cells. AMPK was known to shut down energy-consuming pathways in response to a fall in the ATP/AMP ratio by phosphorylating key enzymes of intermediate metabolism. Here we will discuss the recent evidence implicating AMPK in the regulation of gene expression in mammals, mainly in the liver and in the pancreatic β-cells.</jats:p>
Leclerc I, Viollet B, da Silva Xavier G, et al., 2002, Role of AMP-activated protein kinase in the regulation of gene transcription., Biochem Soc Trans, Vol: 30, Pages: 307-311, ISSN: 0300-5127
Varadi A, Ainscow EK, Allan VJ, et al., 2002, Molecular mechanisms involved in secretory vesicle recruitment to the plasma membrane in β-cells, BIOCHEMICAL SOCIETY TRANSACTIONS, Vol: 30, Pages: 328-332, ISSN: 0300-5127
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- Citations: 9
Nicholls LI, Ainscow EK, Rutter GA, 2002, Glucose-stimulated insulin secretion does not require activation of pyruvate dehydrogenase: Impact of adenovirus-mediated overexpression of PDH kinase and PDH phosphate phosphatase in pancreatic islets, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol: 291, Pages: 1081-1088, ISSN: 0006-291X
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- Citations: 20
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