Publications
673 results found
Kola B, Boscaro M, Rutter GA, et al., 2006, Expanding role of AMPK in endocrinology, TRENDS IN ENDOCRINOLOGY AND METABOLISM, Vol: 17, Pages: 205-215, ISSN: 1043-2760
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- Citations: 170
Varadi A, Grant A, McCormack M, et al., 2006, Intracellular ATP-sensitive K<SUP>+</SUP> channels in mouse pancreatic beta cells:: against a role in organelle cation homeostasis, DIABETOLOGIA, Vol: 49, Pages: 1567-1577, ISSN: 0012-186X
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- Citations: 32
Rutter GA, Hill EV, 2006, Insulin vesicle release: Walk, kiss, pause... then run, PHYSIOLOGY, Vol: 21, Pages: 189-196, ISSN: 1548-9213
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- Citations: 40
Rutter GA, 2006, Moving Ca<SUP>2+</SUP> from the encloplasmic reticulum to mitochondria:: is spatial intimacy enough?, BIOCHEMICAL SOCIETY TRANSACTIONS, Vol: 34, Pages: 351-355, ISSN: 0300-5127
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- Citations: 16
Bell CJ, Rutter GA, Griffiths EJ, 2006, Use of the mitochondrial Ca<SUP>2+</SUP>-transport inhibitors Ru360 and clonazepam to investigate cell Ca<SUP>2+</SUP>-signalling in adult cardiomyocytes:: A cautionary tale, 28th Annual International-Society-for-Heart-Research North American Section Meeting, Publisher: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, Pages: 924-924, ISSN: 0022-2828
Varadi A, Grant A, McCormack M, et al., 2006, Intracellular ATP-sensitive K(+) channels in mouse pancreatic beta cells: against a role in organelle cation homeostasis., Diabetologia, ISSN: 0012-186X
AIMS/HYPOTHESIS: ATP-sensitive K(+) (K(ATP)) channels located on the beta cell plasma membrane play a critical role in regulating insulin secretion and are targets for the sulfonylurea class of antihyperglycaemic drugs. Recent reports suggest that these channels may also reside on insulin-containing dense-core vesicles and mitochondria. The aim of this study was to explore these possibilities and to test the hypothesis that vesicle-resident channels play a role in the control of organellar Ca(2+) concentration or pH. METHODS: To quantify the subcellular distribution of the pore-forming subunit Kir6.2 and the sulfonylurea binding subunit SUR1 in isolated mouse islets and clonal pancreatic MIN6 beta cells, we used four complementary techniques: immunoelectron microscopy, density gradient fractionation, vesicle immunopurification and fluorescence-activated vesicle isolation. Intravesicular and mitochondrial concentrations of free Ca(2+) were measured in intact or digitonin-permeabilised MIN6 cells using recombinant, targeted aequorins, and intravesicular pH was measured with the recombinant fluorescent probe pHluorin. RESULTS: SUR1 and Kir6.2 immunoreactivity were concentrated on dense-core vesicles and on vesicles plus the endoplasmic reticulum/Golgi network, respectively, in both islets and MIN6 cells. Reactivity to neither subunit was detected on mitochondria. Glibenclamide, tolbutamide and diazoxide all failed to affect Ca(2+) uptake into mitochondria, and K(ATP) channel regulators had no significant effect on intravesicular free Ca(2+) concentrations or vesicular pH. CONCLUSIONS/INTERPRETATION: A significant proportion of Kir6.2 and SUR1 subunits reside on insulin-secretory vesicles and the distal secretory pathway in mouse beta cells but do not influence intravesicular ion homeostasis. We propose that dense-core vesicles may serve instead as sorting stations for the delivery of channels to the plasma membrane.
Rutter GA, Varadi A, Tsuboi T, et al., 2006, Insulin secretion in health and disease: genomics, proteomics and single vesicle dynamics, Biochemical Society Transactions, Vol: 34, Pages: 247-250, ISSN: 0300-5127
<jats:p>Defective insulin secretion from pancreatic islet β-cells is a sine qua non of Type II (non-insulin-dependent) diabetes. Digital imaging analysis of the nanomechanics of individual exocytotic events, achieved using total internal reflection fluorescence microscopy, has allowed us to demonstrate that insulin is released via transient or ‘cavicapture’ events whereby the vesicle and plasma membranes fuse transiently and reversibly. Such studies reveal that an increase in the number of abortive fusion events contributes to defective insulin secretion in in vitro models of Type II diabetes. Complementary analyses of genome-wide changes in β-cell gene expression, at both the mRNA and protein levels, are now facilitating the identification of key molecular players whose altered expression may contribute to the secretory defects in the diabetic β-cell.</jats:p>
Rutter GA, Varadi A, Tsuboi T, et al., 2006, Insulin secretion in health and disease: genomics, proteomics and single vesicle dynamics, BIOCHEMICAL SOCIETY TRANSACTIONS, Vol: 34, Pages: 247-250, ISSN: 0300-5127
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- Citations: 11
Tsuboi T, Ravier MA, Parton LE, et al., 2006, Sustained exposure to high glucose concentrations modifies glucose signaling and the mechanics of secretory vesicle fusion in primary rat pancreatic β-cells, DIABETES, Vol: 55, Pages: 1057-1065, ISSN: 0012-1797
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- Citations: 54
Storto M, Capobianco L, Battaglia G, et al., 2006, Insulin secretion is controlled by mGlu5 metabotropic glutamate receptors, MOLECULAR PHARMACOLOGY, Vol: 69, Pages: 1234-1241, ISSN: 0026-895X
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- Citations: 42
Richards SK, Parton LE, Leclerc I, et al., 2005, Over-expression of AMP-activated protein kinase impairs pancreatic β-cell function <i>in vivo</i>, JOURNAL OF ENDOCRINOLOGY, Vol: 187, Pages: 225-235, ISSN: 0022-0795
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- Citations: 79
Tsuboi T, Ravier MA, Xie H, et al., 2005, Mammalian exocyst complex is required for the docking step of insulin vesicle exocytosis, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 280, Pages: 25565-25570, ISSN: 0021-9258
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- Citations: 52
Ravier MA, Rutter GA, 2005, Glucose or insulin, but not zinc ions, inhibit glucagon secretion from mouse pancreatic α-cells, DIABETES, Vol: 54, Pages: 1789-1797, ISSN: 0012-1797
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- Citations: 215
Bell CJ, Rutter GA, Griffiths EJ, 2005, Mitochondrial and cytosolic calcium transients in adult cardiomyocytes detected using targeted aequorin, 25th Annual Scientific Session of the European Section of the International-Society-for-Heart-Research, Publisher: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, Pages: 1004-1005, ISSN: 0022-2828
Varadi A, Tsuboi T, Rutter GA, 2005, Myosin Va transports dense core secretory vesicles in pancreatic MIN6 β-cells, MOLECULAR BIOLOGY OF THE CELL, Vol: 16, Pages: 2670-2680, ISSN: 1059-1524
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- Citations: 125
Gallo A, Ceolotto G, Pinton P, et al., 2005, Metformin prevents glucose-induced protein kinase C-β2 activation in human umbilical vein endothelial cells through an antioxidant mechanism, DIABETES, Vol: 54, Pages: 1123-1131, ISSN: 0012-1797
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- Citations: 86
Richards SK, LeClerc I, Mathieson PW, et al., 2005, The effect of AMP-activated protein kinase on success rate and glycaemic control of murine islet isografts, Annual Meeting of the Association-of-Surgeons-of-Great-Britian-and-Ireland, Publisher: JOHN WILEY & SONS LTD, Pages: 34-34, ISSN: 0007-1323
Guyot AM, Evagelidis A, Gibson C, et al., 2005, Luciferase mutant expressed in HEK cells reveals transmembrane ATP efflux during mechanical stimulation, Experimental Biology 2005 Meeting/35th International Congress of Physiological Sciences, Publisher: FEDERATION AMER SOC EXP BIOL, Pages: A659-A659, ISSN: 0892-6638
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- Citations: 1
Sorto M, Capobianco L, Battaglia G, et al., 2005, Insulin secretion is under the control of type-5 metabotropic glutamate receptors, 5th International Meeting on Metabotropic Glutamate Receptors, Publisher: PERGAMON-ELSEVIER SCIENCE LTD, Pages: 274-274, ISSN: 0028-3908
Diraison F, Ravier MA, Richards SK, et al., 2005, Sterol regulatory element binding protein1 (SREBP1) is required for the potentiation by culture at high glucose concentrations of glucose-stimulated insulin secretion from mouse islets, 41st Annual Meeting of the European-Association-for-the-Study-of-Diabetes, Publisher: SPRINGER, Pages: A165-A166, ISSN: 0012-186X
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- Citations: 1
Ravier MA, Tillmar L, Rutter GA, 2005, Glucose-regulated plasma membrane recruitment of protein kinase C β11 in clonal pancreatic α-cells imaged by confocal and total internal reflection fluorescence microscopy, 65th Annual Meeting of the American-Diabetes-Association, Publisher: AMER DIABETES ASSOC, Pages: A406-A406, ISSN: 0012-1797
Xavier GD, Diraison F, Qian Q, et al., 2005, Carbohydrate response-element binding protein regulates liver-type pyruvate kinase and pancreatic duodenum homeobox-1 expression in MIN6 β-cells by direct and indirect mechanisms, respectively, 41st Annual Meeting of the European-Association-for-the-Study-of-Diabetes, Publisher: SPRINGER, Pages: A157-A157, ISSN: 0012-186X
Rutter GA, Richards SK, Parton LE, et al., 2005, Over-expression of activated AMP-activated protein kinase (AMPK) impairs in vivo pancreatic β-cell function in transplanted pancreatic islets, 41st Annual Meeting of the European-Association-for-the-Study-of-Diabetes, Publisher: SPRINGER, Pages: A88-A88, ISSN: 0012-186X
McKinnon CM, Rutter GA, 2005, Regulation of glucagon gene expression by insulin is abolished after FOXO1 silencing, 41st Annual Meeting of the European-Association-for-the-Study-of-Diabetes, Publisher: SPRINGER, Pages: A160-A160, ISSN: 0012-186X
Richards SK, Parton LE, Leclerc I, et al., 2005, AMP-activated protein kinase (AMPK) activity in murine islet isografts as a determinant of recipient glycaemic control, 65th Annual Meeting of the American-Diabetes-Association, Publisher: AMER DIABETES ASSOC, Pages: A480-A480, ISSN: 0012-1797
Diraison F, Motakis E, Parton LE, et al., 2004, Impact of adenoviral transduction with SREBP1c or AMPK on pancreatic islet gene expression profile - Analysis with oligonucleotide microarrays, DIABETES, Vol: 53, Pages: S84-S91, ISSN: 0012-1797
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- Citations: 28
Leclerc I, Rutter GA, 2004, AMP-activated protein kinase:: A new β-cell glucose sensor?: Regulation by amino acids and calcium ions, DIABETES, Vol: 53, Pages: S67-S74, ISSN: 0012-1797
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- Citations: 74
Varadi A, Cirulli V, Rutter GA, 2004, Mitochondrial localization as a determinant of capacitative Ca<SUP>2+</SUP> entry in HeLa cells, CELL CALCIUM, Vol: 36, Pages: 499-508, ISSN: 0143-4160
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- Citations: 51
Tsuboi T, McMahon HT, Rutter GA, 2004, Mechanisms of dense core vesicle recapture following "kiss and run" ("cavicapture") exocytosis in insulin-secreting cells, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 279, Pages: 47115-47124, ISSN: 0021-9258
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- Citations: 151
Da Silva Xavier G, Qian Q, Cullen PJ, et al., 2004, Erratum: Distinct roles for insulin and insulin-like growth factor-1 receptors in pancreatic β-cell glucose sensing revealed by RNA silencing (Biochemical Journal (2004)377 (149-158)), Biochemical Journal, Vol: 383, ISSN: 0264-6021
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