Imperial College London


Faculty of MedicineDepartment of Immunology and Inflammation

Visiting Professor



+44 (0)20 7594 1190g.screaton Website




Ms Claire Puddephatt +44 (0)20 7594 1190




2.15Faculty BuildingSouth Kensington Campus






BibTex format

author = {Yacoub, S and Lam, PK and Huynh, TT and Nguyen, Ho HH and Dong, Thi HT and Van, NT and Lien, LT and Ha, QNT and Le, DHT and Mongkolspaya, J and Culshaw, A and Yeo, TW and Wertheim, H and Simmons, C and Screaton, G and Wills, B},
doi = {cid/cix567},
journal = {Clinical Infectious Diseases},
pages = {1453--1461},
title = {Endothelial nitric oxide pathways in the pathophysiology of dengue: a prospective observational study.},
url = {},
volume = {65},
year = {2017}

RIS format (EndNote, RefMan)

AB - Background: Dengue can cause increased vascular permeability that may lead to hypovolemic shock. Endothelial dysfunction may underlie this; however the association of endothelial nitric oxide pathways with disease severity is unknown. Methods: We performed a prospective observational study in two Vietnamese hospitals, assessing patients presenting early (<72 hours fever) and patients hospitalized with warning signs or severe dengue. The reactive hyperaemic index (RHI), which measures endothelium-dependent vasodilation and is a surrogate marker of endothelial function and NO bioavailability was evaluated using peripheral artery tonometry (EndoPAT) and plasma levels of L-arginine, Arginase-1 and ADMA were measured at serial time-points. The main outcome of interest was plasma leakage severity. Results: 314 patients were enrolled, median age of the participants was 21 (IQR 13-30) years. No difference was found in the endothelial parameters between dengue and other febrile illness (OFI). Considering dengue patients, the RHI was significantly lower for patients with severe plasma leakage compared to those with no leakage (1.46 vs. 2.00, P<0.001), over acute time-points, apparent already in the early febrile phase (1.29 vs. 1.75, P=0.012). RHI correlated negatively with arginase-1, and positively with L-arginine (P=0.001). Endothelial dysfunction/NO bioavailability is associated with worse plasma leakage, occurs early in dengue illness and correlates with hypoargininaemia and high arginase-1 levels.
AU - Yacoub,S
AU - Lam,PK
AU - Huynh,TT
AU - Nguyen,Ho HH
AU - Dong,Thi HT
AU - Van,NT
AU - Lien,LT
AU - Mongkolspaya,J
AU - Culshaw,A
AU - Yeo,TW
AU - Wertheim,H
AU - Simmons,C
AU - Screaton,G
AU - Wills,B
DO - cid/cix567
EP - 1461
PY - 2017///
SN - 1058-4838
SP - 1453
TI - Endothelial nitric oxide pathways in the pathophysiology of dengue: a prospective observational study.
T2 - Clinical Infectious Diseases
UR -
UR -
VL - 65
ER -