Please see the Rudenko laboratory website for a full description of our research: http://rudenkolab.co.uk/
My research group studies African trypanosomes, which cause African Sleeping Sickness in sub-Saharan Africa. African trypanosomes are single-celled organisms which multiply extracellularly in the bloodstream of the infected mammalian host. Trypanosomes are very adept at changing their Variant Surface Glycoprotein (VSG) surface coat, allowing them to continuously escape from antibody attack during the course of a chronic infection. This continuous switching of the VSG surface coat is referred to as antigenic variation. We are studying the molecular mechanisms mediating antigenic variation in African trypanosomes. We are interested in how this protective VSG coat is synthesised, and how VSG protein is monitored during the trypanosome cell cycle. In addition, we are interested in how the transcription of VSG is regulated. Individual trypanosomes have many hundreds of VSG genes, of which only one VSG variant is expressed at a time. We are currently investigating how this stringent transcriptional control is mediated.
I am a Wellcome Senior Research Fellow/ Professor in Molecular Microbiology. I have an M.Sc in Biochemistry from the University of Leiden, the Netherlands and a Ph.D in Molecular Biology from the University of Amsterdam on the basis of research performed in the Department of Genetics, Columbia University, New York, NY. I did postdoctoral research on antigenic variation in African trypanosomes in the laboratory of Prof. Piet Borst at the Netherlands Cancer Institute, Amsterdam. I came to the UK as a Wellcome Senior Fellow in the Basic Biomedical Sciences at the University of Oxford, where I set up a research group in the Peter Medawar Building for Pathogen Research with an academic affiliation with the Department of Biochemistry.
My research group is located in the Sir Alexander Fleming Building on the Imperial College-South Kensington Campus.
et al., The role of genomic location and flanking 3’UTR in the generation of functional levels of Variant Surface Glycoprotein in Trypanosoma brucei, Molecular Microbiology, ISSN:0950-382X
et al., 2017, Selective inhibition of RNA polymerase I transcription as a potential approach to treat African trypanosomiasis, Plos Neglected Tropical Diseases, Vol:11, ISSN:1935-2735
et al., 2017, Well-positioned nucleosomes punctuate polycistronic pol II transcription units and flank silent VSG gene arrays in Trypanosoma brucei, Epigenetics & Chromatin, Vol:10, ISSN:1756-8935
et al., 2016, Blocking Synthesis of the Variant Surface Glycoprotein Coat in Trypanosoma brucei Leads to an Increase in Macrophage Phagocytosis Due to Reduced Clearance of Surface Coat Antibodies, Plos Pathogens, Vol:12, ISSN:1553-7366
et al., 2015, Identification of the ISWI chromatin remodeling complex of the early branching eukaryote Trypanosoma brucei, Journal of Biological Chemistry, Vol:290, ISSN:0021-9258, Pages:26954-26967