Fungal gene regulation
Emeritus Professor Herb Arst’s group does basic research on the control of gene expression using the model filamentous fungus Aspergillus nidulans. Current research interest is focused on the regulation of gene expression by ambient pH and calcium signalling and homeostasis. The pH regulatory system controls expression of genes involved in metabolite (eg toxins and antibiotics) export, extracellular enzymes and nutrient uptake and its functioning is crucial for fungal infections of both plants and animals. The calcium project originated from the identification of a novel mutational combination which results in strains requiring high levels of calcium for growth and an indicator activity which reflects calcium status. Work done elsewhere indicates that perturbation of calcium signalling/homeostasis is likely to prevent fungal virulence as well.
A mechanistic model showing a number of features of the pH regulatory system in Aspergillus.
Reprinted from Trends in Genetics 19, H.N. Arst, Jr. & M.A. Penalva, pH regulation in Aspergillus and parallels with higher eukaryotic regulatory systems, pp 224-231, 2003 with permission from Elsevier.
et al., 2019, Characterization of Aspergillus nidulans TRAPPs uncovers unprecedented similarities between fungi and metazoans and reveals the modular assembly of TRAPPII, Plos Genetics, Vol:15, ISSN:1553-7390
et al., 2018, Endocytic recycling via the TGN underlies the polarized hyphal mode of growth, Plos Genetics, Vol:14, ISSN:1553-7390
et al., 2017, Genetic studies on the physiological role of CORVET in Aspergillus nidulans., Fems Microbiology Letters, Vol:364, ISSN:0378-1097
Arst HN, Mellado L, Espeso EA, 2016, Proteolytic activation of both components of the cation-stress responsive Slt pathway in Aspergillus nidulans, Molecular Biology of the Cell, Vol:27, ISSN:1939-4586, Pages:2598-2612
et al., Mutational analysis of the Aspergillus ambient pH receptor PalH underscores its potential as a target for antifungal compounds, Molecular Microbiology, ISSN:1365-2958