Imperial College London
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ProfessorHectorKeun

Faculty of Medicine, Department of Surgery & Cancer

Professor of Biochemistry
 
 
 
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Contact

 

+44 (0)20 7594 3161h.keun

 
 
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Location

 

officesInstitute of Reproductive and Developmental BiologyHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Lau:2017:10.1038/srep46079,
author = {Lau, C-HE and Tredwell, GD and Ellis, JK and Lam, EW-F and Keun, HC},
doi = {10.1038/srep46079},
journal = {SCIENTIFIC REPORTS},
title = {Metabolomic characterisation of the effects of oncogenic PIK3CA transformation in a breast epithelial cell line},
url = {http://dx.doi.org/10.1038/srep46079},
volume = {7},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Somatic mutations in PIK3CA are frequently found in a number of human cancers, including breast cancer, altering cellular physiology and tumour sensitivity to chemotherapy. This renders PIK3CA an attractive molecular target for early detection and personalised therapy. Using 1H Nuclear Magnetic Resonance spectroscopy (NMR) and Gas Chromatography – Mass Spectrometery (GC-MS) together with 13C stable isotope-labelled glucose and glutamine as metabolic tracers, we probed the phenotypic changes in metabolism following a single copy knock-in of mutant PIK3CA (H1047R) in the MCF10A cell line, an important cell model for studying oncogenic transformation in breast tissues. We observed effects in several metabolic pathways, including a decrease in glycerophosphocholine level together with increases in glutaminolysis, de novo fatty acid synthesis and pyruvate entry into the tricarboxylic acid cycle. Our findings highlight altered glyceroplipid metabolism and lipogenesis, as key metabolic phenotypes of mutant PIK3CA transformation that are recapitulated in the MCF10A cellular model.
AU  - Lau,C-HE
AU  - Tredwell,GD
AU  - Ellis,JK
AU  - Lam,EW-F
AU  - Keun,HC
DO  - 10.1038/srep46079
PY  - 2017///
SN  - 2045-2322
TI  - Metabolomic characterisation of the effects of oncogenic PIK3CA transformation in a breast epithelial cell line
T2  - SCIENTIFIC REPORTS
UR  - http://dx.doi.org/10.1038/srep46079
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000398970300001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/48205
VL  - 7
ER  -
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