Publications
495 results found
TAGUCHI T, SEKO A, KITAJIMA K, et al., 1993, PRECISE STRUCTURAL DETERMINATION OF UNIQUE HIGHLY BRANCHED MULTIANTENNARY N-GLYCAN UNITS PRESENT IN FISH EGG HYOSOPHORIN, GLYCOCONJUGATE JOURNAL, Vol: 10, Pages: 280-280, ISSN: 0282-0080
SICILIANO RA, MORRIS HR, MCDOWELL RA, et al., 1993, STRUCTURAL CHARACTERIZATION OF THE SULFATED SACCHARIDE EPITOPES OF BOVINE PROOPIOMELANOCORTIN, GLYCOCONJUGATE JOURNAL, Vol: 10, Pages: 278-278, ISSN: 0282-0080
DUFFIN KL, LANGE GW, WELPLY JK, et al., 1993, IDENTIFICATION AND OLIGOSACCHARIDE STRUCTURE-ANALYSIS OF RHODOPSIN GLYCOFORMS CONTAINING GALACTOSE AND SIALIC-ACID, GLYCOBIOLOGY, Vol: 3, Pages: 365-380, ISSN: 0959-6658
- Author Web Link
- Cite
- Citations: 25
KHOO KH, MORRIS HR, MCDOWELL RA, et al., 1993, FABMS DERIVATIZATION STRATEGIES FOR THE ANALYSIS OF HEPARIN-DERIVED OLIGOSACCHARIDES, CARBOHYDRATE RESEARCH, Vol: 244, Pages: 205-223, ISSN: 0008-6215
- Author Web Link
- Cite
- Citations: 33
SICILIANO RA, MORRIS HR, MCDOWELL RA, et al., 1993, THE LEWIS-X EPITOPE IS A MAJOR NONREDUCING STRUCTURE IN THE SULFATED N-GLYCANS ATTACHED TO ASN-65 OF BOVINE PROOPIOMELANOCORTIN, GLYCOBIOLOGY, Vol: 3, Pages: 225-239, ISSN: 0959-6658
- Author Web Link
- Cite
- Citations: 42
SAMBASIVAM H, RASSOULI M, MURRAY RK, et al., 1993, STUDIES ON THE CARBOHYDRATE MOIETY AND ON THE BIOSYNTHESIS OF RAT C-REACTIVE PROTEIN, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 268, Pages: 10007-10016, ISSN: 0021-9258
- Author Web Link
- Cite
- Citations: 15
YAMADA SH, YOSHIDA K, SUGIURA M, et al., 1993, STRUCTURAL STUDIES ON THE BACTERIAL LYASE-RESISTANT TETRASACCHARIDES DERIVED FROM THE ANTITHROMBIN-III-BINDING SITE OF PORCINE INTESTINAL HEPARIN, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 268, Pages: 4780-4787, ISSN: 0021-9258
- Author Web Link
- Cite
- Citations: 90
TAGUCHI T, SEKO A, KITAJIMA K, et al., 1993, STRUCTURAL STUDIES OF A NOVEL TYPE OF TETRAANTENNARY SIALOGLYCAN UNIT IN A CARBOHYDRATE-RICH GLYCOPEPTIDE ISOLATED FROM THE FERTILIZED-EGGS OF INDIAN MEDAKA FISH, ORYZIAS-MELASTIGMA, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 268, Pages: 2353-2362, ISSN: 0021-9258
- Author Web Link
- Cite
- Citations: 42
BESRA GS, MCNEIL MR, RIVOIRE B, et al., 1993, FURTHER STRUCTURAL DEFINITION OF A NEW FAMILY OF GLYCOPEPTIDOLIPIDS FROM MYCOBACTERIUM-XENOPI, BIOCHEMISTRY, Vol: 32, Pages: 347-355, ISSN: 0006-2960
- Author Web Link
- Cite
- Citations: 21
MCNEIL M, BESRA GS, CHATTERJEE D, et al., 1993, CHEMICAL AND ENZYMATIC METHODS DECIPHER INTRICATE NONREPEATING STRUCTURES IN MYCOBACTERIAL CELL-WALL POLYSACCHARIDES, JOURNAL OF CELLULAR BIOCHEMISTRY, Pages: 9-9, ISSN: 0730-2312
Siddiqui KS, Rangarajan M, Hartley BS, et al., 1993, Arthrobacter D-xylose isomerase: partial proteolysis with thermolysin., Biochem J, Vol: 289 ( Pt 1), Pages: 201-208, ISSN: 0264-6021
The pattern and kinetics of partial proteolysis of Arthrobacter D-xylose isomerase tetramer was studied in order to determine the flexibility of surface loops that may control its stability. It was completely resistant to trypsin, chymotrypsin and elastase at 37 degrees C, but thermolysin cleaved specifically and quantitatively at Thr-347-Leu-348 between helices 10 and 11 to remove 47 residues from the C-terminus of each 43.3 kDa subunit. At high temperatures, helices 9 and 10 were removed from each 38 kDa subunit to give a 36 kDa tetramer. The kinetics of nicking by thermolysin indicated that the Thr-347-Leu-348 loop is locked at low temperatures, but 'melts' at 25 degrees C and is fully flexible above 34 degrees C. The flexibility appears to be associated with binding of Ca2+ ions at the active site, since Co2+, Mg2+ and xylitol protect in proportion to their ability to displace Ca2+. The missing C-terminal helices make many intersubunit contacts that appear in the structure to stabilize the tetramer, but the properties of the purified nicked proteins are almost indistinguishable from the native enzyme. Both the 38 kDa tetramer and the 36 kDa tetramer are identically active and dissociate similarly in urea or SDS to fully active dimers, but the nicked dimers are slightly less stable to urea at 62 degrees C. In the Mg2+ form the thermostability of the 38 kDa tetramer is identical with that of the native enzyme, but the 36 kDa tetramer has a slightly lower 'melting point' (70 degrees C versus 80 degrees C), which may be due to unravelling from the end of helix 8. Since elimination of all the C-terminal helices and many intersubunit contacts has so little effect, one can conclude that the 'weak point' that controls the protein's thermostability lies within the N-terminal beta-barrel domain.
SIDDIQUI KS, RANGARAJAN M, HARTLEY BS, et al., 1993, ARTHROBACTER D-XYLOSE ISOMERASE - PARTIAL PROTEOLYSIS WITH THERMOLYSIN, BIOCHEMICAL JOURNAL, Vol: 289, Pages: 201-208, ISSN: 0264-6021
- Author Web Link
- Cite
- Citations: 17
KHOO KH, MORRIS HR, DELL A, 1993, STRUCTURAL CHARACTERIZATION OF THE MAJOR GLYCANS OF TOXOCARA-CANIS ES ANTIGENS, Symposium on Toxocariasis, at the 1992 Spring Meeting of the British-Society-for-Parasitology, Publisher: INST BIOLOGY, Pages: 133-140
- Author Web Link
- Cite
- Citations: 9
DEWARDENER HE, HOLLAND S, ALAGHBANDZADEH J, et al., 1993, A POSSIBLE CONNECTION BETWEEN AN INCREASED CONCENTRATION OF A CYTOCHEMICALLY DETECTABLE SUBSTANCE IN THE HYPOTHALAMUS OF THE SPONTANEOUSLY HYPERTENSIVE RAT AND CERTAIN CEREBRAL CHOLINERGIC DISTURBANCES, SATELLITE MEETING ON NATRIURETIC FACTORS AND HYPERTENSION, AT THE 14TH SCIENTIFIC MEETING OF THE INTERNATIONAL-SOC-OF-HYPERTENSION, Publisher: LIPPINCOTT-RAVEN PUBL, Pages: S109-S111, ISSN: 0160-2446
- Author Web Link
- Cite
- Citations: 2
de Wardener HE, Holland S, Alaghband-Zadeh J, et al., 1993, A possible connection between an increased concentration of a cytochemically detectable substance in the hypothalamus of the spontaneously hypertensive rat and certain cerebral cholinergic disturbances., J Cardiovasc Pharmacol, Vol: 22 Suppl 2, Pages: S109-S111, ISSN: 0160-2446
The hypothalamus of the spontaneously hypertensive rat (SHR) contains an increased concentration of a cytochemically detectable choline-like substance, the physicochemical properties of which are similar to the choline analogue dimethyl methylene immonium. In the hypothalamus of the adult SHR there is an increased turnover of acetylcholine in the cholinergic pressor posterior hypothalamic nucleus and the neurons of the dorsomedial cholinergic depressor nucleus are atrophic, possibly due to diminished cholinergic activity. Both of these changes are pressor. It is suggested that an increased hypothalamic content of a choline-like analogue might either be responsible for, or an indicator of, these abnormalities.
REASON AJ, MORRIS HR, PANICO M, et al., 1992, LOCALIZATION OF O-GLCNAC MODIFICATION ON THE SERUM RESPONSE TRANSCRIPTION FACTOR, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 267, Pages: 16911-16921, ISSN: 0021-9258
- Author Web Link
- Cite
- Citations: 119
SINGH A, BLENCH I, MORRIS HR, et al., 1992, SYNERGISTIC INTERACTION OF GROWTH-FACTORS AND ALBUMIN IN REGULATING ESTRADIOL SYNTHESIS IN BREAST-CANCER CELLS, MOLECULAR AND CELLULAR ENDOCRINOLOGY, Vol: 85, Pages: 165-173, ISSN: 0303-7207
- Author Web Link
- Cite
- Citations: 27
MORRIS HR, 1992, ACTION ON ACADEMIC PAY, NATURE, Vol: 356, Pages: 10-10, ISSN: 0028-0836
- Author Web Link
- Cite
- Citations: 3
OLDS RJ, LANE DA, CASO R, et al., 1992, ANTITHROMBIN-III BUDAPEST - A SINGLE AMINO-ACID SUBSTITUTION (429PRO TO LEU) IN A REGION HIGHLY CONSERVED IN THE SERPIN FAMILY, BLOOD, Vol: 79, Pages: 1206-1212, ISSN: 0006-4971
- Author Web Link
- Cite
- Citations: 51
RASSOULI M, SAMBASIVAM H, AZADI P, et al., 1992, DERIVATION OF THE AMINO-ACID-SEQUENCE OF RAT C-REACTIVE PROTEIN FROM CDNA CLONING WITH ADDITIONAL STUDIES ON THE NATURE OF ITS DIMERIC COMPONENT, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 267, Pages: 2947-2954, ISSN: 0021-9258
- Author Web Link
- Cite
- Citations: 36
GALADARI SHI, MORRIS HR, DIMARZO V, 1992, THE EFFECT OF A CAMP ANALOG ON CA-2+ IONOPHORE-INDUCED, ANTIGEN-INDUCED AND AGONIST-INDUCED INOSITOL PHOSPHATE RELEASE IN RAT BASOPHILIC LEUKEMIA (RBL-1) CELLS, BIOCHIMICA ET BIOPHYSICA ACTA, Vol: 1133, Pages: 218-222, ISSN: 0006-3002
- Author Web Link
- Cite
- Citations: 2
ROQUEMORE EP, DELL A, MORRIS HR, et al., 1992, VERTEBRATE LENS ALPHA-CRYSTALLINS ARE MODIFIED BY O-LINKED N-ACETYLGLUCOSAMINE, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 267, Pages: 555-563, ISSN: 0021-9258
- Author Web Link
- Cite
- Citations: 98
SINGH A, GHILCHIK MW, PATEL SR, et al., 1992, IDENTIFICATION OF ALBUMIN IN BREAST-TUMOR CYTOSOL AS A FACTOR INVOLVED IN THE STIMULATION OF ESTRADIOL 17-BETA-HYDROXYSTEROID DEHYDROGENASE (REDUCTIVE) ACTIVITY, MOLECULAR AND CELLULAR ENDOCRINOLOGY, Vol: 83, Pages: 85-92, ISSN: 0303-7207
- Author Web Link
- Cite
- Citations: 18
REASON AJ, DELL A, MORRIS HR, et al., 1991, CHARACTERIZATION OF THE N-LINKED OLIGOSACCHARIDES OF THE LIGHT CHAIN OF HUMAN GLYCOPROTEIN IIB BY FAB-MS, CARBOHYDRATE RESEARCH, Vol: 221, Pages: 169-177, ISSN: 0008-6215
- Author Web Link
- Cite
- Citations: 2
Reason AJ, Blench IP, Haltiwanger RS, et al., 1991, High-sensitivity FAB-MS strategies for O-GlcNAc characterization., Glycobiology, Vol: 1, Pages: 585-594, ISSN: 0959-6658
In this paper we report the first application of fast atom bombardment mass spectrometry (FAB-MS) to O-linked N-acetylglucosamine (O-GlcNAc)-bearing glycopeptides. Using N-acetylgalactosamine (GalNAc)- and Gal-GalNAc-containing glycopeptides (isolated from Tn glycophorin and desialylated normal glycophorin, respectively) as readily available model compounds, rapid and sensitive derivatization/FAB-MS strategies applicable to serine/threonine-rich glycopeptides have been devised. Peptides and glycopeptides were propionylated in a 1 min reaction using a mixture of trifluoroacetic anhydride and propionic acid, and the product mixtures were analysed directly by FAB-MS. Glycopeptides and peptides rich in hydroxylated residues afforded characteristic clusters of molecular ions at high sensitivity. Additional sensitivity enhancement was achieved by prior esterification of carboxyl groups. These methods were used in a study of O-GlcNAc glycopeptides produced by purified O-GlcNAc transferase addition of GlcNAc to the synthetic peptides YSDSPSTST and YSGSPSTST in which Y is tyrosine, S is serine, D is aspartic acid, P is proline, T is threonine and G is glycine. The propionyl derivatives afforded high-quality spectra which unequivocally showed that the majority of the glycopeptides were substituted with a single GlcNAc residue. Low pmol quantities of material gave detectable signals. The propionylation/FAB-MS procedure has been combined with gas-phase sequencing strategies and shows promise for defining the sites of glycosylation of O-GlcNAc glycopeptides that are available in limited quantities.
IRELAND H, LANE DA, THOMPSON E, et al., 1991, ANTITHROMBIN GLASGOW-II - ALANINE-382 TO THREONINE MUTATION IN THE SERPIN P12 POSITION, RESULTING IN A SUBSTRATE REACTION WITH THROMBIN, BRITISH JOURNAL OF HAEMATOLOGY, Vol: 79, Pages: 70-74, ISSN: 0007-1048
- Author Web Link
- Cite
- Citations: 34
Alvarez FJ, Morris HR, Priestley JV, 1991, Sub-populations of smaller diameter trigeminal primary afferent neurons defined by expression of calcitonin gene-related peptide and the cell surface oligosaccharide recognized by monoclonal antibody LA4., J Neurocytol, Vol: 20, Pages: 716-731, ISSN: 0300-4864
Immunocytochemistry has been used to examine the trigeminal ganglion cell populations in the rat which expresses calcitonin gene-related peptide (CGRP) and the oligosaccharide antigen recognized by the monoclonal antibody LA4. Calcitonin gene-related peptide and LA4 identify two large but mainly separate populations of trigeminal ganglion cells. Depending on the method of assessment used, CGRP-immunoreactive cells represent 29-37% of trigeminal ganglion cells while LA4 labels 26-40% of the cells, but with only 8% overlap between the two populations. Both CGRP and LA4 label predominantly small diameter cells (mean diameters 23 microns and 25 microns respectively) but with CGRP cells exhibiting a greater range of diameters than LA4 cells. The cell sizes indicate that small diameter CGRP-immunoreactive cells and most LA4-immunoreactive cells are likely to have unmyelinated axons, and together the two populations can account for the great majority of unmyelinated trigeminal primary afferent neurons. Centrally, CGRP and LA4 show distinct patterns of staining. Thus although both antigens are found in lamina II of subnucleus caudalis of the spinal trigeminal nucleus, CGRP is most abundant in lamina I and lamina II outer while LA4 immunoreactivity is most dense in lamina II inner. In addition CGRP-, but not LA4-, immunoreactive fibres occur in the magnocellular portion of caudalis. Previous studies have show that in rat dorsal root ganglion cells CGRP coexists with most other known neuropeptides and can therefore be used as a general marker for peptide-containing primary afferents. In contrast LA4 labels a cell population which is probably largely identical to that identified by the presence of fluoride resistant acid phosphatase or by the binding of lectins such as Griffonia simplicifolia isolectin B4 and this population does not contain neuropeptides. Our results thus provide further evidence that unmyelinated primary afferents can be divided into peptide and non-peptide
Chan AL, Morris HR, Panico M, et al., 1991, A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator., Glycobiology, Vol: 1, Pages: 173-185, ISSN: 0959-6658
We have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins.
DELL A, MORRIS HR, GREER F, et al., 1991, FAST-ATOM-BOMBARDMENT MASS-SPECTROMETRY OF SULFATED OLIGOSACCHARIDES FROM OVINE LUTROPIN, CARBOHYDRATE RESEARCH, Vol: 209, Pages: 33-50, ISSN: 0008-6215
- Author Web Link
- Cite
- Citations: 27
CASO R, LANE DA, THOMPSON EA, et al., 1991, ANTITHROMBIN VICENZA, ALA-384 TO PRO (GCA TO CCA) MUTATION, TRANSFORMING THE INHIBITOR INTO A SUBSTRATE, BRITISH JOURNAL OF HAEMATOLOGY, Vol: 77, Pages: 87-92, ISSN: 0007-1048
- Author Web Link
- Cite
- Citations: 45
This data is extracted from the Web of Science and reproduced under a licence from Thomson Reuters. You may not copy or re-distribute this data in whole or in part without the written consent of the Science business of Thomson Reuters.