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BibTex format

@article{Pang:2011:10.1126/science.1207438,
author = {Pang, P and Chiu, PCN and Lee, C and Chang, L and Panico, M and Morris, HR and Haslam, SM and Khoo, K and Clark, GF and Yeung, WSB and Dell, A},
doi = {10.1126/science.1207438},
journal = {Science},
pages = {1761--1764},
title = {Human Sperm Binding Is Mediated by the Sialyl-Lewisx Oligosaccharide on the Zona Pellucida},
url = {http://dx.doi.org/10.1126/science.1207438},
volume = {333},
year = {2011}
}

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TY  - JOUR
AB  - Human fertilization begins when spermatozoa bind to the extracellular matrix coating of the oocyte, known as the zona pellucida (ZP). One spermatozoan then penetrates this matrix and fuses with the egg cell, generating a zygote. Although carbohydrate sequences on the ZP have been implicated in sperm binding, the nature of the ligand was unknown. Here, ultrasensitive mass spectrometric analyses revealed that the sialyl-Lewisx sequence [NeuAcα2-3Galβ1-4(Fucα1-3)GlcNAc], a well-known selectin ligand, is the most abundant terminal sequence on the N- and O-glycans of human ZP. Sperm-ZP binding was largely inhibited by glycoconjugates terminated with sialyl-Lewisx sequences or by antibodies directed against this sequence. Thus, the sialyl-Lewisx sequence represents the major carbohydrate ligand for human sperm-egg binding.
AU  - Pang,P
AU  - Chiu,PCN
AU  - Lee,C
AU  - Chang,L
AU  - Panico,M
AU  - Morris,HR
AU  - Haslam,SM
AU  - Khoo,K
AU  - Clark,GF
AU  - Yeung,WSB
AU  - Dell,A
DO  - 10.1126/science.1207438
EP  - 1764
PY  - 2011///
SP  - 1761
TI  - Human Sperm Binding Is Mediated by the Sialyl-Lewisx Oligosaccharide on the Zona Pellucida
T2  - Science
UR  - http://dx.doi.org/10.1126/science.1207438
UR  - http://www.sciencemag.org/content/333/6050/1761.abstract
UR  - http://hdl.handle.net/10044/1/15584
VL  - 333
ER  -

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Emeritus Professor Howard R. Morris FRS

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