Imperial College London

DrIainDunlop

Faculty of EngineeringDepartment of Materials

Senior Lecturer in Biomaterials
 
 
 
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Contact

 

+44 (0)20 7594 6731i.dunlop

 
 
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Location

 

1.02Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

24 results found

Gonzalez-Carter DA, Ong ZY, McGilvery CM, Dunlop IE, Dexter DT, Porter AEet al., 2019, L-DOPA functionalized, multi-branched gold nanoparticles as brain-targeted nano-vehicles, NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE, Vol: 15, Pages: 1-11, ISSN: 1549-9634

JOURNAL ARTICLE

Wu P-J, Kabakova IV, Ruberti JW, Sherwood JM, Dunlop IE, Paterson C, Torok P, Overby DRet al., 2018, Water content, not stiffness, dominates Brillouin spectroscopy measurements in hydrated materials, NATURE METHODS, Vol: 15, Pages: 561-562, ISSN: 1548-7091

JOURNAL ARTICLE

Loftus C, Saeed M, Davis DM, Dunlop IEet al., 2018, Activation of Human Natural Killer Cells by Graphene Oxide-Templated Antibody Nanoclusters, NANO LETTERS, Vol: 18, Pages: 3282-3289, ISSN: 1530-6984

JOURNAL ARTICLE

Luongo G, Campagnolo P, Perez JE, Kosel J, Georgiou TK, Regoutz A, Payne DJ, Stevens MM, Ryan MP, Porter AE, Dunlop IEet al., 2017, Scalable High-Affinity Stabilization of Magnetic Iron Oxide Nanostructures by a Biocompatible Antifouling Homopolymer, ACS APPLIED MATERIALS & INTERFACES, Vol: 9, Pages: 40059-40069, ISSN: 1944-8244

JOURNAL ARTICLE

Ong ZY, Chen S, Nabavi E, Regoutz A, Payne DJ, Elson DS, Dexter DT, Dunlop IE, Porter AEet al., 2017, Multibranched Gold Nanoparticles with Intrinsic LAT-1 Targeting Capabilities for Selective Photothermal Therapy of Breast Cancer, ACS APPLIED MATERIALS & INTERFACES, Vol: 9, Pages: 39259-39270, ISSN: 1944-8244

JOURNAL ARTICLE

Delcassian D, Sattler S, Dunlop IE, 2017, T cell immunoengineering with advanced biomaterials, INTEGRATIVE BIOLOGY, Vol: 9, Pages: 211-222, ISSN: 1757-9694

JOURNAL ARTICLE

Podhorska L, Delcassian D, Goode AE, Agyei M, McComb DW, Ryan MP, Dunlop IEet al., 2016, Mechanisms of Polymer-Templated Nanoparticle Synthesis: Contrasting ZnS and Au, LANGMUIR, Vol: 32, Pages: 9216-9222, ISSN: 0743-7463

JOURNAL ARTICLE

Perez JE, Contreras MF, Vilanova E, Felix LP, Margineanu MB, Luongo G, Porter AE, Dunlop IE, Ravasi T, Kosel Jet al., 2016, Cytotoxicity and intracellular dissolution of nickel nanowires., Nanotoxicology, Vol: 10, Pages: 871-880

The assessment of cytotoxicity of nanostructures is a fundamental step for their development as biomedical tools. As widely used nanostructures, nickel nanowires (Ni NWs) seem promising candidates for such applications. In this work, Ni NWs were synthesized and then characterized using vibrating sample magnetometry, energy dispersive X-Ray analysis, and electron microscopy. After exposure to the NWs, cytotoxicity was evaluated in terms of cell viability, cell membrane damage, and induced apoptosis/necrosis on the model human cell line HCT 116. The influence of NW to cell ratio (10:1 to 1000:1) and exposure times up to 72 hours was analyzed for Ni NWs of 5.4 μm in length, as well as for Ni ions. The results show that cytotoxicity markedly increases past 24 hours of incubation. Cellular uptake of NWs takes place through the phagocytosis pathway, with a fraction of the dose of NWs dissolved inside the cells. Cell death results from a combination of apoptosis and necrosis, where the latter is the outcome of the secondary necrosis pathway. The cytotoxicity of Ni ions and Ni NWs dissolution studies suggest a synergistic toxicity between NW aspect ratio and dissolved Ni, with the cytotoxic effects markedly increasing after 24 hours of incubation.

JOURNAL ARTICLE

Gonzalez-Carter D, Goode AE, Fiammengo R, Dunlop IE, Dexter DT, Porter AEet al., 2016, Inhibition of Leptin-ObR Interaction Does not Prevent Leptin Translocation Across a Human Blood-Brain Barrier Model., J Neuroendocrinol, Vol: 28

The adipocyte-derived hormone leptin regulates appetite and energy homeostasis through the activation of leptin receptors (ObR) on hypothalamic neurones; hence, leptin must be transported through the blood-brain barrier (BBB) to reach its target sites in the central nervous system. During obesity, however, leptin BBB transport is decreased, in part precluding leptin as a viable clinical therapy against obesity. Although the short isoform of the ObR (ObRa) has been implicated in the transport of leptin across the BBB as a result of its elevated expression in cerebral microvessels, accumulating evidence indicates that leptin BBB transport is independent of ObRa. In the present study, we employed an ObR-neutralising antibody (9F8) to directly examine the involvement of endothelial ObR in leptin transport across an in vitro human BBB model composed of the human endothelial cell line hCMEC/D3. Our results indicate that, although leptin transport across the endothelial monolayer was nonparacellular, and energy- and endocytosis-dependent, it was not inhibited by pre-treatment with 9F8, despite the ability of the latter to recognise hCMEC/D3-expressed ObR, prevent leptin-ObR binding and inhibit leptin-induced signal transducer and activator of transcription 3 (STAT-3) phosphorylation in hCMEC/D3 cells. Furthermore, hCMEC/D3 cells expressed the transporter protein low-density lipoprotein receptor-related protein-2 (LRP-2), which is capable of binding and endocytosing leptin. In conclusion, our results demonstrate that leptin binding to and signalling through ObR is not required for efficient transport across human endothelial monolayers, indicating that ObR is not the primary leptin transporter at the human BBB, a role which may fall upon LRP-2. A deeper understanding of leptin BBB transport will help clarify the exact causes for leptin resistance seen in obesity and aid in the development of more efficient BBB-penetrating leptin analogues.

JOURNAL ARTICLE

Harrison RH, Steele JAM, Chapman R, Gormley AJ, Chow LW, Mahat MM, Podhorska L, Palgrave RG, Payne DJ, Hettiaratchy SP, Dunlop IE, Stevens MMet al., 2015, Modular and Versatile Spatial Functionalization of Tissue Engineering Scaffolds through Fiber-Initiated Controlled Radical Polymerization, ADVANCED FUNCTIONAL MATERIALS, Vol: 25, Pages: 5748-5757, ISSN: 1616-301X

JOURNAL ARTICLE

Dunlop IE, Ryan MP, Goode AE, Schuster C, Terrill NJ, Weaver JVMet al., 2014, Direct synthesis of PEG-encapsulated gold nanoparticles using branched copolymer nanoreactors, RSC ADVANCES, Vol: 4, Pages: 27702-27707, ISSN: 2046-2069

JOURNAL ARTICLE

Delcassian D, Depoil D, Rudnicka D, Liu M, Davis DM, Dustin ML, Dunlop IEet al., 2013, Nanoscale Ligand Spacing Influences Receptor Triggering in T Cells and NK Cells, NANO LETTERS, Vol: 13, Pages: 5608-5614, ISSN: 1530-6984

JOURNAL ARTICLE

Leo BF, Chen S, Kyo Y, Herpoldt K-L, Terrill NJ, Dunlop IE, McPhail DS, Shaffer MS, Schwander S, Gow A, Zhang J, Chung KF, Tetley TD, Porter AE, Ryan MPet al., 2013, The Stability of Silver Nanoparticles in a Model of Pulmonary Surfactant, ENVIRONMENTAL SCIENCE & TECHNOLOGY, Vol: 47, Pages: 11232-11240, ISSN: 0013-936X

JOURNAL ARTICLE

Shahal T, Geiger B, Dunlop IE, Spatz JPet al., 2012, Regulation of Integrin Adhesions by Varying the Density of Substrate-Bound Epidermal Growth Factor, BIOINTERPHASES, Vol: 7, ISSN: 1934-8630

JOURNAL ARTICLE

Dunlop IE, Thomas RK, Titmus S, Osborne V, Edmondson S, Huck WTS, Klein Jet al., 2012, Structure and Collapse of a Surface-Grown Strong Polyelectrolyte Brush on Sapphire, LANGMUIR, Vol: 28, Pages: 3187-3193, ISSN: 0743-7463

JOURNAL ARTICLE

Dunlop IE, Briscoe WH, Titmuss S, Jacobs RMJ, Osborne VL, Edmondson S, Huck WTS, Klein Jet al., 2009, Direct Measurement of Normal and Shear Forces between Surface-Grown Polyelectrolyte Layers, JOURNAL OF PHYSICAL CHEMISTRY B, Vol: 113, Pages: 3947-3956, ISSN: 1520-6106

JOURNAL ARTICLE

Dunlop IE, Zorn S, Richter G, Srot V, Kelsch M, van Aken PA, Skoda M, Gerlach A, Spatz JP, Schreiber Fet al., 2009, Titanium-silicon oxide film structures for polarization-modulated infrared reflection absorption spectroscopy, THIN SOLID FILMS, Vol: 517, Pages: 2048-2054, ISSN: 0040-6090

JOURNAL ARTICLE

Dunlop IE, Spatz JP, Dustin ML, 2009, Degenerative diseases: manipulating the micro- and nanoscale architecture regulates immunological synapse function, Nanotechnolgy, Editors: Vogel, Weinheim, Publisher: Wiley-VCH

BOOK CHAPTER

Sugiharto AB, Johnson CM, Dunlop IE, Roke Set al., 2008, Delocalized surface modes reveal three-dimensional structures of complex biomolecules, JOURNAL OF PHYSICAL CHEMISTRY C, Vol: 112, Pages: 7531-7534, ISSN: 1932-7447

JOURNAL ARTICLE

Briscoe WH, Chen M, Dunlop IE, Klein J, Penfold J, Jacobs RMJet al., 2007, Applying grazing incidence X-ray reflectometry (XRR) to characterising nanofilms on mica, JOURNAL OF COLLOID AND INTERFACE SCIENCE, Vol: 306, Pages: 459-463, ISSN: 0021-9797

JOURNAL ARTICLE

Perkin S, Chai L, Kampf N, Raviv U, Briscoe W, Dunlop I, Titmuss S, seo MS, Kumacheva E, Klein Jet al., 2006, Forces between mica surfaces, prepared in different ways, across aqueous and nonaqueous liquids confined to molecularly thin films, LANGMUIR, Vol: 22, Pages: 6142-6152, ISSN: 0743-7463

JOURNAL ARTICLE

Dunlop IE, Briscoe WH, Titmuss S, Sakellariou G, Hadjichristidis N, Klein Jet al., 2004, Interactions between polymer brushes: Varying the number of end-attaching groups, MACROMOLECULAR CHEMISTRY AND PHYSICS, Vol: 205, Pages: 2443-2450, ISSN: 1022-1352

JOURNAL ARTICLE

Titmuss S, Briscoe WH, Dunlop IE, Sakellariou G, Hadjichristidis N, Klein Jet al., 2004, Effect of end-group sticking energy on the properties of polymer brushes: Comparing experiment and theory, JOURNAL OF CHEMICAL PHYSICS, Vol: 121, Pages: 11408-11419, ISSN: 0021-9606

JOURNAL ARTICLE

Krebs MRH, MacPhee CE, Miller AF, Dunlop IE, Dobson CM, Donald AMet al., 2004, The formation of spherulites by amyloid fibrils of bovine insulin, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 101, Pages: 14420-14424, ISSN: 0027-8424

JOURNAL ARTICLE

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