Imperial College London
Portrait Picture

ProfessorIanGould

Faculty of Natural Sciences, Department of Chemistry

Senior Research Investigator
 
 
 
//

Contact

 

+44 (0)20 7594 5809i.gould

 
 
//

Location

 

110BMolecular Sciences Research HubWhite City Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Toroz:2019:10.1021/acsomega.8b02271,
author = {Toroz, D and Khanna, T and Gould, I},
doi = {10.1021/acsomega.8b02271},
journal = {ACS Omega},
pages = {3341--3350},
title = {Modelling the effect of BSEP inhibitors in lipid bilayers by means of all atom Molecular Dynamics (MD) simulation},
url = {http://dx.doi.org/10.1021/acsomega.8b02271},
volume = {4},
year = {2019}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The human bile salt export pump (BSEP) is a membrane protein expressed on the canalicular plasma membrane domain of hepatocytes, which mediates active transport of unconjugated and conjugated bile salts from liver cells into bile. Genetically inherited defects in BSEP expression or activity causes cholestatic liver injury, and many drugs that cause cholestatic drug-induced liver injury (DILI) in humans have been shown to inhibit BSEP activity in vitro and in vivo, suggesting this could be one of the mechanisms that initiates human DILI. The relationship between BSEP inhibition and molecular physicochemical properties has been previously investigated identifying calculated lipophilicity and molecular weight to be significantly correlated with BSEP inhibition. Predictive BSEP classification models, constructed through multiple quantitative structure-activity relationship modeling approaches, exhibit significant anomalies with differences in experimental IC50 values of three orders of magnitude for molecules of the same calculated lipophilicity and molecular weight. The interaction of these molecules with the lipid bilayer membrane has been identified as a major contributory factor to BSEP inhibition. In this study we apply unbiased molecular dynamics (MD) simulations to study the permeation times as well as orientation preferences of BSEP inhibitors in two different lipids (saturated DMPC and unsaturated POPC). The simulations reveal that strong BSEP inhibitors have the slowest permeation times, in both POPC and DMPC, with a secondary conclusion that the time of permeation is more rapid in POPC than DMPC. The orientation of the molecules in the membrane reveals strong correlation with chemical structure, molecules containing only hydroxyl and carboxylic groups orient themselves perpendicular to the membrane whereas molecules containing nitrogen atoms exhibit no orientational preference in respect of the membrane. Finally, H-bonding interactions computed between the mo
AU  - Toroz,D
AU  - Khanna,T
AU  - Gould,I
DO  - 10.1021/acsomega.8b02271
EP  - 3350
PY  - 2019///
SN  - 2470-1343
SP  - 3341
TI  - Modelling the effect of BSEP inhibitors in lipid bilayers by means of all atom Molecular Dynamics (MD) simulation
T2  - ACS Omega
UR  - http://dx.doi.org/10.1021/acsomega.8b02271
UR  - http://hdl.handle.net/10044/1/66985
VL  - 4
ER  -
Download