Imperial College London
Alternate

ProfessorIanWilson

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Visiting Professor
 
 
 
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i.wilson

 
 
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Location

 

311Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{King:2020:10.1016/j.chroma.2019.460597,
author = {King, AM and Trengove, RD and Mullin, LG and Rainville, PD and Isaac, G and Plumb, RS and Gethings, LA and Wilson, ID},
doi = {10.1016/j.chroma.2019.460597},
journal = {Journal of Chromatography A},
pages = {1--10},
title = {Rapid profiling method for the analysis of lipids in human plasma using ion mobility enabled-reversed phase-ultra high performance liquid chromatography/mass spectrometry},
url = {http://dx.doi.org/10.1016/j.chroma.2019.460597},
volume = {1611},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The incorporation of ion mobility (IM) into LC–MS analysis has been demonstrated to result in the generation of superior quality MS and MS/MS spectral data as well as providing enhanced resolution in the IM dimension based on lipid class. Here a sub 4 min microbore LC-ion mobility-accurate mass MS (LC-IM-MS) method has been developed for the rapid, profiling of lipids in biological fluids. The method was scaled directly from a conventional, 12 min, LC-MS analysis maintaining the chromatographic performance and lipid separation observed in the longer methodology giving a 75% saving in mobile phase consumption and analysis time. Because of the additional dimension of separation provided by IM, improvements in mass spectral quality from the increased resolution of co-eluting species were also seen when compared to the same separation without IM, thus aiding the identification of target lipids. When applied to human plasma samples some 5037 (positive ESI) and 2020 (negative ESI) mass/retention time features were detected following adduct deconvolution and, of these, 3727 and 800 of those present in the pooled plasma QC samples had a CV of below 30% for positive and negative ESI modes respectively. The method was applied to the analysis of a pilot set of commercially sourced breast cancer plasma samples enabling the differentiation of samples from healthy controls and patients based on their lipid phenotypes. Analysis of the resulting data showed that phosphatidylcholines, triglycerides and diglycerides exhibited lower expression and phosphatidylserine showed increased expression in the breast cancer samples compared to those of healthy subjects. The coefficients of variation, determined by reference to the QC data, for all of the features identified as potential markers of disease, were 6% or less.
AU  - King,AM
AU  - Trengove,RD
AU  - Mullin,LG
AU  - Rainville,PD
AU  - Isaac,G
AU  - Plumb,RS
AU  - Gethings,LA
AU  - Wilson,ID
DO  - 10.1016/j.chroma.2019.460597
EP  - 10
PY  - 2020///
SN  - 0021-9673
SP  - 1
TI  - Rapid profiling method for the analysis of lipids in human plasma using ion mobility enabled-reversed phase-ultra high performance liquid chromatography/mass spectrometry
T2  - Journal of Chromatography A
UR  - http://dx.doi.org/10.1016/j.chroma.2019.460597
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000510312700015&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.sciencedirect.com/science/article/pii/S0021967319310052?via%3Dihub
UR  - http://hdl.handle.net/10044/1/77671
VL  - 1611
ER  -
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