Imperial College London
Alternate

ProfessorIanWilson

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Visiting Professor
 
 
 
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Contact

 

i.wilson

 
 
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Location

 

311Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Gika:2019:10.1016/j.jchromb.2019.04.009,
author = {Gika, H and Virgiliou, C and Theodoridis, G and Plumb, RS and Wilson, ID},
doi = {10.1016/j.jchromb.2019.04.009},
journal = {Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences},
pages = {136--147},
title = {Untargeted LC/MS-based metabolic phenotyping (metabonomics/metabolomics): The state of the art},
url = {http://dx.doi.org/10.1016/j.jchromb.2019.04.009},
volume = {1117},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Liquid chromatography (LC) hyphenated to mass spectrometry is currently the most widely used means of determining metabolic phenotypes via both untargeted and targeted analysis. At present a range of analytical separations, including reversed-phase, hydrophilic interaction and ion-pair LC are employed to maximise metabolome coverage with ultra (high) performance liquid chromatography (UHPLC) increasingly displacing conventional high performance liquid chromatography because of the need for short analysis times and high peak capacity in such applications. However, it is widely recognized that these methodologies do not entirely solve the problems facing researchers trying to perform comprehensive metabolic phenotyping and in addition to these “routine” approaches there are continuing investigations of alternative separation methods including 2-dimensional/multi column approaches. These involve either new stationary phases or multidimensional combinations of the more conventional materials currently used, as well as application of miniaturization or “new” approaches such as supercritical HP and UHP- chromatographic separations. There is also a considerable amount of interest in the combination of chromatographic and ion mobility separations, with the latter providing both an increase in resolution and the potential to provide additional structural information via the determination of molecular collision cross section data. However, key problems remain to be solved including ensuring quality, comparability across different laboratories and the ever present difficulty of identifying unknowns.
AU  - Gika,H
AU  - Virgiliou,C
AU  - Theodoridis,G
AU  - Plumb,RS
AU  - Wilson,ID
DO  - 10.1016/j.jchromb.2019.04.009
EP  - 147
PY  - 2019///
SN  - 1570-0232
SP  - 136
TI  - Untargeted LC/MS-based metabolic phenotyping (metabonomics/metabolomics): The state of the art
T2  - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
UR  - http://dx.doi.org/10.1016/j.jchromb.2019.04.009
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000467891000014&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.sciencedirect.com/science/article/pii/S1570023219301370?via%3Dihub
UR  - http://hdl.handle.net/10044/1/77701
VL  - 1117
ER  -
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