Imperial College London
Alternate

ProfessorIanWilson

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Visiting Professor
 
 
 
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Contact

 

i.wilson

 
 
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Location

 

311Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{King:2019:10.1021/acs.jproteome.9b00334,
author = {King, A and Baginski, M and Morikawa, Y and Rainville, PD and Gethings, LA and Wilson, ID and Plumb, RS},
doi = {10.1021/acs.jproteome.9b00334},
journal = {Journal of Proteome Research},
pages = {4055--4064},
title = {Application of a novel mass spectral data acquisition approach to lipidomic analysis of liver extracts from sitaxentan-treated liver-humanized PXB mice.},
url = {http://dx.doi.org/10.1021/acs.jproteome.9b00334},
volume = {18},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The application of a data-independent acquisition (DIA) method ("SONAR") that employs a rapidly scanning quadrupole is described for the lipidomic analysis of complex biological extracts. Using this approach, the MS acquisition window can be varied between 1 and 25 Da, enabling the isolation of ions prior to their entering the collision cell. By rapidly scanning the resolving quadrupole window over a specified mass range, co-eluting precursor ions are transmitted sequentially into the collision cell, where collision energies are cycled between low and elevated levels to induce fragmentation. This method of data generation provides both precursor and fragment ion information at high specificity, allowing for greater accuracy of compound identification, whether using a database, spectral libraries, or comparison to authentic standards. The value of the approach in simplifying and "de-cluttering" the spectra of co-eluting lipids is shown with examples from lipidomic profiles obtained in investigations of the composition of organic extracts of livers obtained from SCID and chimeric liver-humanized mice administered under various experimental conditions.
AU  - King,A
AU  - Baginski,M
AU  - Morikawa,Y
AU  - Rainville,PD
AU  - Gethings,LA
AU  - Wilson,ID
AU  - Plumb,RS
DO  - 10.1021/acs.jproteome.9b00334
EP  - 4064
PY  - 2019///
SN  - 1535-3893
SP  - 4055
TI  - Application of a novel mass spectral data acquisition approach to lipidomic analysis of liver extracts from sitaxentan-treated liver-humanized PXB mice.
T2  - Journal of Proteome Research
UR  - http://dx.doi.org/10.1021/acs.jproteome.9b00334
UR  - https://www.ncbi.nlm.nih.gov/pubmed/31550900
UR  - https://pubs.acs.org/doi/10.1021/acs.jproteome.9b00334
UR  - http://hdl.handle.net/10044/1/77651
VL  - 18
ER  -
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