Imperial College London
Alternate

ProfessorIanWilson

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Visiting Professor
 
 
 
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Contact

 

i.wilson

 
 
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Location

 

311Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Gray:2017:10.1021/acs.analchem.6b04623,
author = {Gray, N and Zia, R and King, A and Patel, VC and Wendon, J and McPhail, MJW and Coen, M and Plumb, RS and Wilson, ID and Nicholson, JK},
doi = {10.1021/acs.analchem.6b04623},
journal = {Analytical Chemistry},
pages = {2478--2487},
title = {High speed quantitative UPLC-MS analysis of multiple amines in human plasma and serum via pre-column derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate: Application to acetaminophen-induced liver failure},
url = {http://dx.doi.org/10.1021/acs.analchem.6b04623},
volume = {89},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - A targeted reversed-phase gradient UPLC-MS/MS assay has been developed for the quantification/monitoring of amino acids and amino-containing compounds in human plasma and serum using pre-column derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AccQTag UltraTM). Derivatization of the target amino-containing compounds reagent required minimal sample preparation and resulted in analytes with excellent chromatographic and mass spectrometric properties. The resulting method, which requires only 10 µl of sample, provides the reproducible and robust separation of 66 analytes in 7.5 minutes, including baseline resolution of isomers such as e.g. leucine and isoleucine. The assay has been validated for the quantification of 33 amino compounds (predominantly amino acids) over a concentration range from 2-20 and 800µM. Intra- and inter-day accuracy of between 0.05-15.6 and 0.78 -13.7 % and precision between 0.91-16.9 % and 2.12-15.9 % were obtained. A further 33 biogenic amines can be monitored in samples for relative changes in concentration rather than quantification. Application of the assay to samples derived from healthy controls and patients suffering from acetaminophen (APAP, paracetamol) induced acute liver failure (ALF) showed significant differences in the amounts of aromatic and branched chain amino acids between the groups as well as a number of other analytes, including the novel observation of increased concentrations of sarcosine in ALF patients. The properties of the developed assay, including short analysis time, make it suitable for high throughput targeted UPLC-ESI-MS/MS metabonomic analysis in clinical and epidemiological environments.
AU  - Gray,N
AU  - Zia,R
AU  - King,A
AU  - Patel,VC
AU  - Wendon,J
AU  - McPhail,MJW
AU  - Coen,M
AU  - Plumb,RS
AU  - Wilson,ID
AU  - Nicholson,JK
DO  - 10.1021/acs.analchem.6b04623
EP  - 2487
PY  - 2017///
SN  - 1520-6882
SP  - 2478
TI  - High speed quantitative UPLC-MS analysis of multiple amines in human plasma and serum via pre-column derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate: Application to acetaminophen-induced liver failure
T2  - Analytical Chemistry
UR  - http://dx.doi.org/10.1021/acs.analchem.6b04623
UR  - http://hdl.handle.net/10044/1/44533
VL  - 89
ER  -
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