ProfessorJaneMitchell

Jordan KB, Evans TW, Mitchell JA, 1998, Production of the isoprostane, 8-iso PGF₂α by human pulmonary artery smooth muscle cells is cyclooxygenase-2-dependent, BRITISH JOURNAL OF PHARMACOLOGY, Vol: 123, Pages: U100-U100, ISSN: 0007-1188

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Jourdan KB, Evans TW, Mitchell JA, 1998, Interleukin-1β inhibits proliferation of human pulmonary artery smooth muscle cells via a cyclooxygenase-2 dependent pathway, BRITISH JOURNAL OF PHARMACOLOGY, Vol: 123, ISSN: 0007-1188

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• Citations: 1

Cirino G, Saunders M, Belvisi MG, Jourdan KB, Evans TW, Coppini A, Mitchell JAet al., 1998, Relative potencies of nonsteroidal anti-inflammatory drugs on purified cyclo-oxygenase-1 and -2, BRITISH JOURNAL OF PHARMACOLOGY, Vol: 123, Pages: U101-U101, ISSN: 0007-1188

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Chabot F, Mitchell JA, Gutteridge JMC, Evans TWet al., 1998, Reactive oxygen species in acute lung injury, EUROPEAN RESPIRATORY JOURNAL, Vol: 11, Pages: 745-757, ISSN: 0903-1936

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• Citations: 289

Mitchell JA, Bishop-Bailey D, Evans TW, Giembycz MA, Belvisi MG, Chivers S, Williams TJ, Pepper JRet al., 1998, Release of neutrophil activating cytokines by human arterial and venous smooth muscle cells, BRITISH JOURNAL OF PHARMACOLOGY, Vol: 123, Pages: U56-U56, ISSN: 0007-1188

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Niebauer J, Webb-Peploe KM, Jourdan K, Mitchell JA, Quinlan GJ, Coats AJSet al., 1998, Chronic exercise training modulates oxidative stress in patients with chronic heart failure, JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, Vol: 31, Pages: 509A-509A, ISSN: 0735-1097

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Brett SJ, Quinlan GJ, Mitchell J, Pepper JR, Evans TWet al., 1998, Production of nitric oxide during surgery involving cardiopulmonary bypass, CRITICAL CARE MEDICINE, Vol: 26, Pages: 272-278, ISSN: 0090-3493

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• Citations: 52

Lu YT, Liu SF, Mitchell JA, Malik AB, Hellewell PG, Evans TWet al., 1998, The role of endogenous nitric oxide in modulating ischemia-reperfusion injury in the isolated, blood-perfused rat lung, AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, Vol: 157, Pages: 273-279, ISSN: 1073-449X

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• Citations: 36

John M, Au BT, Jose PJ, Lim S, Saunders M, Barnes PJ, Mitchell JA, Belvisi MG, Chung KFet al., 1998, Expression and release of interleukin-8 by human airway smooth muscle cells: Inhibition by Th-2 cytokines and corticosteroids, AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, Vol: 18, Pages: 84-90, ISSN: 1044-1549

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• Citations: 146

Patel HJ, Mitchell JA, Chung FK, Belvisi MGet al., 1998, Endogenous nitric oxide suppresses growth of human ASM cells, NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, Vol: 358, Pages: R310-R310, ISSN: 0028-1298

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Woods M, Bishop-Bailey D, Pepper TR, Evans TW, Mitchell TA, Warner TDet al., 1998, Cytokine and lipopolysaccharide stimulation of endothelin-1 release from human internal mammary artery and saphenous vein smooth-muscle cells, JOURNAL OF CARDIOVASCULAR PHARMACOLOGY, Vol: 31, Pages: S348-S350, ISSN: 0160-2446

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• Citations: 27

Jourdan KB, Mitchell JA, Evans TW, 1997, Co-induction of iNOS and COX-2 in P.A. smooth muscle cells: A comparison between rat and human, Thorax, Vol: 52, ISSN: 0040-6376

Introduction Nitric oxide synthase (NOS) and cyclooxygenase (COX) enzymes exist in both inducible and constitutive forms (iNOS and COX-2). They are induced by inflammatory mediators such as cytokines and endotoxin, levels of which are elevated during conditions such as sepsis and ARDS. We have looked at their co-induction in isolated pulmonary artery (PA) smooth muscle cells. Methods PA smooth muscle cells from both rat and human tissue were cultured by explant in culture medium containing 20% foetal calf serum. PGE2 was measured by radioimmuno assay and nitrite was measured by the Greiss assay. Results Both rat (open columns) and human (hatched columns) cells released PGE2 after treatment with IL-1 β. LPS did not stimulate a release of PGE2 from human cells but did induce a release from rat cells. (fig A). Both LPS and IL-1β stimulated the release of nitrite from rat cells but not from the human cells (fig B). In all cases the release of nitrite was completely inhibited by aminoguanidine the specific iNOS inhibitor (Griffiths MJ et al Am J Respir Crit Care Med 152(5), 1599-1604 (1995)), and PGE2 release was inhibited by the specific COX-2 inhibitor L-745,337 (Chan C-C et al J Pharmacol and Exp Ther 274, 1531-1537 (1995)). (Graph Presented) Conclusions The inducible forms of NOS and COX are co-expressed in rat pulmonary artery smooth muscle cells after treatment with IL-1β and LPS. By contrast human PA smooth muscle cells expressed detectable COX-2 but not iNOS. Thus, iNOS may be expressed in relatively low amounts and modulate vascular function differently in human than in rat cells. These observations have direct clinical relevance to diseases such as sepsis where iNOS and COX-2 have been implicated.

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Jourdan KB, Evans TW, Quinlan G, Mitchell JAet al., 1997, Oxidant stress induces the release of both PGE<inf>2</inf> and 8-iso PGF<inf>2α</inf> by a COX-2 dependent pathway, Thorax, Vol: 52, ISSN: 0040-6376

Introduction 8-iso prostaglandin (PG) F2α is one of a series of prostaglandin-like compounds the isoprostanes. 8-iso PGF2α has been shown to have potent vasoactive properties on both rat and human pulmonary artery (PA). Their synthesis was initially described as independent of the enzyme cyclooxygenase and through free radical action on arachidonic acid, although recent evidence suggests the involvement of cyclooxygenase. We have compared the release of PGE2 (a typical COX metabolite) and 8-iso PGF2α from human PA smooth muscles cells subjected to oxidant stress by a combination of xanthine oxidase (XO) and its substrate hypoxanthine (HX). Methods Human PA smooth muscle cells were cultured by explant from human PA denuded of endothelium in culture medium containing 20% foetal calf serum. PGE2 was measured by radioimmumoassay and 8-iso PGF2α by enzyme immunoassay. Cell respiration was measured by the conversion of 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide to a blue formazan product. Results 8-iso PGF2α was increased approximately two-fold after incubation with XO and it's substrate HX compared to XO alone. This ratio of increase was the same for PGE2 (fig. A). Oxidant stress was confirmed by a reduction of cellular respiration in the presence of XO and HX (fig. B). The release of both the prostanoids was inhibited more than 80% by the COX-2 specific inhibitor L-745,337. Similar observations were made when prostanoid release was stimulated by cytokines. (Graph Presented) Conclusion Oxidant stress stimulates the release of the isoporstane, 8-iso PGF2α and the typical COX metabolite, PGE2. Moreover, COX-2 appears to be important in the release of both prostanoids. These observations may help to explain why prostaglandin and isoprostane release is increased in conditions of oxidant stress.

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Mitchell JA, Belvisi MG, 1997, Too many COX (cyclo-oxygenase) spoil the broth: aspirin-sensitive asthma and 5-lipoxygenase, THORAX, Vol: 52, Pages: 933-935, ISSN: 0040-6376

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• Citations: 18

BishopBailey D, Pepper JR, Larkin S, Evans T, Mitchell JAet al., 1997, Interleukin-1 beta induces anti-proliferative prostanoids via cyclo-oxygenase-2 in human saphenous vein and internal mammary artery smooth muscle cells, BRITISH JOURNAL OF PHARMACOLOGY, Vol: 122, Pages: P20-P20, ISSN: 0007-1188

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• Citations: 2

BishopBailey D, BurkeGaffney A, Hellewell PG, Pepper JR, Mitchell JAet al., 1997, Interleukin (IL)-1 beta-induced cyclo-oxygenase-2 activity inhibits IL-1 beta-induced expression of ICAM-1-and IL-4-induced expression of VCAM-1 on saphenous vein smooth muscle cells, BRITISH JOURNAL OF PHARMACOLOGY, Vol: 122, Pages: P21-P21, ISSN: 0007-1188

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• Citations: 1

Woods M, BishopBailey D, Pepper JR, Evans TW, Mitchell JA, Warner TDet al., 1997, Cytokine and LPS stimulation of endothelin-1 release from internal mammary artery and saphenous vein smooth muscle cells, BRITISH JOURNAL OF PHARMACOLOGY, Vol: 122, Pages: P18-P18, ISSN: 0007-1188

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BishopBailey D, Pepper JR, Larkin S, Evans T, Mitchell JAet al., 1997, Differential induction of cyclo-oxygenase-2 in human arterial and venous smooth muscle cells: Role of endogenous prostanoids, BRITISH JOURNAL OF PHARMACOLOGY, Vol: 122, Pages: P19-P19, ISSN: 0007-1188

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• Citations: 1

Hamilton LC, Mitchell JA, Warner TD, 1997, Induction of COX-2 in vivo leads to greatly increased production of 6-keto-PGF(1 alpha) following administration of exogenous arachidonic acid or bradykinin, BRITISH JOURNAL OF PHARMACOLOGY, Vol: 122, Pages: P22-P22, ISSN: 0007-1188

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• Citations: 3

BishopBailey D, Pepper JR, Haddad EB, Newton R, Larkin SW, Mitchell JAet al., 1997, Induction of cyclooxygenase-2 in human saphenous vein and internal mammary artery, ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, Vol: 17, Pages: 1644-1648, ISSN: 1079-5642

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• Citations: 59

Griffiths MJD, Curzen NP, Mitchell JA, Evans TWet al., 1997, In vivo treatment with endotoxin increases rat pulmonary vascular contractility despite NOS induction, AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, Vol: 156, Pages: 654-658, ISSN: 1073-449X

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• Citations: 21

Mitchell JA, Williams FM, Williams TJ, Larkin SWet al., 1997, Role of nitric oxide in the dilator actions of capsaicin-sensitive nerves in the rabbit coronary circulation., Neuropeptides, Vol: 31, Pages: 333-338, ISSN: 0143-4179

Perivascular sensory nerves release calcitonin gene-related peptide (CGRP) and substance P, the dilator actions of which can be regulated by nitric oxide (NO). This study investigated the role of NO in the vasodilation caused by sensory nerve stimulation, by capsaicin, or exogenous CGRP and substance P in the isolated perfused coronary circulation of the rabbit. Coronary perfusion pressure (CPP) was raised in order to observe vasodilator responses, using the thromboxane mimetic, U46619. Capsaicin (3 x 10(-6) moles), alpha CGRP (3 x 10(-11) moles) and substance P (3 x 10(-12) moles) caused comparable reductions in CCP. At these concentrations, responses to capsaicin and CGRP were inhibited by the antagonist CGRP(8-37) but unaffected by the neurokinin-1 receptor antagonist, CP 96,345. The nitric oxide synthase inhibitor, NG nitro L-arginine methyl ester inhibited the effects of substance P and capsaicin but not CGRP. These results suggest that CGRP release following capsaicin-induced sensory nerve activation is modulated by NO.

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Mitchell JA, Saunders M, Barnes PJ, Newton R, Belvisi MGet al., 1997, Sodium salicylate inhibits cyclo-oxygenase-2 activity independently of transcription factor (nuclear factor kappa B) activation: Role of arachidonic acid, MOLECULAR PHARMACOLOGY, Vol: 51, Pages: 907-912, ISSN: 0026-895X

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• Citations: 110

Chabot F, Mitchell JA, Quinlan GJ, Evans TWet al., 1997, Characterization of the vasodilator properties of peroxynitrite on rat pulmonary artery: Role of poly(adenosine 5'-diphosphoribose) synthase, BRITISH JOURNAL OF PHARMACOLOGY, Vol: 121, Pages: 485-490, ISSN: 0007-1188

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• Citations: 51

BishopBailey D, Larkin SW, Warner TD, Chen G, Mitchell JAet al., 1997, Characterization of the induction of nitric oxide synthase and cyclo-oxygenase in rat aorta in organ culture, BRITISH JOURNAL OF PHARMACOLOGY, Vol: 121, Pages: 125-133, ISSN: 0007-1188

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• Citations: 96

Jourdan KB, Mitchell JA, Evans TW, 1997, Release of isoprostanes by human pulmonary artery in organ culture: A cyclo-oxygenase and nitric oxide dependent pathway, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol: 233, Pages: 668-672, ISSN: 0006-291X

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• Citations: 49

Jourdan KB, Evans TW, Curzen NP, Mitchell JAet al., 1997, Evidence for a dilator function of 8-iso prostaglandin F-2 alpha in rat pulmonary artery, BRITISH JOURNAL OF PHARMACOLOGY, Vol: 120, Pages: 1280-1285, ISSN: 0007-1188

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• Citations: 44

BishopBailey D, Haddad EB, Larkin S, Newton R, Pepper JR, Evans TW, Mitchell JAet al., 1997, Induction of cyclo-oxygenase-2 in human internal mammary artery and saphenous vein in organ culture, BRITISH JOURNAL OF PHARMACOLOGY, Vol: 120, Pages: P72-P72, ISSN: 0007-1188

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Jourdan KB, Curzen NP, Evans TW, Mitchell JAet al., 1997, The isoprostane 8-iso prostaglandin F-2 alpha vasodilates rat pulmonary artery via the release of nitric oxide, BRITISH JOURNAL OF PHARMACOLOGY, Vol: 120, Pages: P166-P166, ISSN: 0007-1188

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Belvisi MG, Saunders MA, Haddad EB, Hirst SJ, Yacoub MH, Barnes PJ, Mitchell JAet al., 1997, Induction of cyclo-oxygenase-2 by cytokines in human cultured airway smooth muscle cells: Novel inflammatory role of this cell type, BRITISH JOURNAL OF PHARMACOLOGY, Vol: 120, Pages: 910-916, ISSN: 0007-1188

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• Citations: 153