Imperial College London

DrJanineBosse

Faculty of MedicineDepartment of Infectious Disease

Senior Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 1803j.bosse

 
 
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Location

 

234Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Li:2018:10.1080/21505594.2018.1491255,
author = {Li, Y and Spiropoulos, J and Cooley, J and Khara, J and Gladstone, C and Asai, M and Bosse, J and Robertson, B and Newton, SM and Langford, P},
doi = {10.1080/21505594.2018.1491255},
journal = {Virulence},
pages = {1126--1137},
title = {Galleria mellonella - a novel infection model for the Mycobacterium tuberculosis complex},
url = {http://dx.doi.org/10.1080/21505594.2018.1491255},
volume = {9},
year = {2018}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Animal models have long been used in tuberculosis research to understand disease pathogenesis and to evaluate novel vaccine candidates and anti-mycobacterial drugs. However, all have limitations and there is no single animal model which mimics all the aspects of mycobacterial pathogenesis seen in humans. Importantly mice, the most commonly used model, do not normally form granulomas, the hallmark of tuberculosis infection. Thus there is an urgent need for the development of new alternative in vivo models. The insect larvae, Galleria mellonella has been increasingly used as a successful, simple, widely available and cost-effective model to study microbial infections. Here we report for the first time that G. mellonella can be used as an infection model for members of the M. tuberculosis complex. We demonstrate a dose-response for G. mellonella survival infected with different inocula of bioluminescent, Mycobacterium bovis BCG lux, and demonstrate suppression of mycobacterial luminesence over 14 days. Histopathology staining and transmission electron microscopy of infected G. mellonella phagocytic haemocytes show internalization and aggregation of M. bovis BCG lux in granuloma-like structures, and increasing accumulation of lipid bodies within M. bovis BCG lux over time, characteristic of latent tuberculosis infection. Our results demonstrate that G. mellonella can act as a surrogate host to study the pathogenesis of mycobacterial infection and shed light on host-mycobacteria interactions, including latent tuberculosis infection
AU - Li,Y
AU - Spiropoulos,J
AU - Cooley,J
AU - Khara,J
AU - Gladstone,C
AU - Asai,M
AU - Bosse,J
AU - Robertson,B
AU - Newton,SM
AU - Langford,P
DO - 10.1080/21505594.2018.1491255
EP - 1137
PY - 2018///
SN - 2150-5594
SP - 1126
TI - Galleria mellonella - a novel infection model for the Mycobacterium tuberculosis complex
T2 - Virulence
UR - http://dx.doi.org/10.1080/21505594.2018.1491255
UR - http://hdl.handle.net/10044/1/61321
VL - 9
ER -