Imperial College London

DrJanineBosse

Faculty of MedicineDepartment of Infectious Disease

Senior Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 1803j.bosse

 
 
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Location

 

234Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Maglennon:2013:10.1186/1297-9716-44-124,
author = {Maglennon, GA and Cook, BS and Deeney, AS and Bossé, JT and Peters, SE and Langford, PR and Maskell, DJ and Tucker, AW and Wren, BW and Rycroft, AN and BRaDP1T, consortium},
doi = {10.1186/1297-9716-44-124},
journal = {Veterinary Research},
title = {Transposon mutagenesis in Mycoplasma hyopneumoniae using a novel mariner-based system for generating random mutations.},
url = {http://dx.doi.org/10.1186/1297-9716-44-124},
volume = {44},
year = {2013}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Mycoplasma hyopneumoniae is the cause of enzootic pneumonia in pigs, a chronic respiratory disease associated with significant economic losses to swine producers worldwide. The molecular pathogenesis of infection is poorly understood due to the lack of genetic tools to allow manipulation of the organism and more generally for the Mycoplasma genus. The objective of this study was to develop a system for generating random transposon insertion mutants in M. hyopneumoniae that could prove a powerful tool in enabling the pathogenesis of infection to be unraveled. A novel delivery vector was constructed containing a hyperactive C9 mutant of the Himar1 transposase along with a mini transposon containing the tetracycline resistance cassette, tetM. M. hyopneumoniae strain 232 was electroporated with the construct and tetM-expressing transformants selected on agar containing tetracycline. Individual transformants contained single transposon insertions that were stable upon serial passages in broth medium. The insertion sites of 44 individual transformants were determined and confirmed disruption of several M. hyopneumoniae genes. A large pool of over 10 000 mutants was generated that should allow saturation of the M. hyopneumoniae strain 232 genome. This is the first time that transposon mutagenesis has been demonstrated in this important pathogen and could be generally applied for other Mycoplasma species that are intractable to genetic manipulation. The ability to generate random mutant libraries is a powerful tool in the further study of the pathogenesis of this important swine pathogen.
AU - Maglennon,GA
AU - Cook,BS
AU - Deeney,AS
AU - Bossé,JT
AU - Peters,SE
AU - Langford,PR
AU - Maskell,DJ
AU - Tucker,AW
AU - Wren,BW
AU - Rycroft,AN
AU - BRaDP1T,consortium
DO - 10.1186/1297-9716-44-124
PY - 2013///
SN - 1297-9716
TI - Transposon mutagenesis in Mycoplasma hyopneumoniae using a novel mariner-based system for generating random mutations.
T2 - Veterinary Research
UR - http://dx.doi.org/10.1186/1297-9716-44-124
UR - http://hdl.handle.net/10044/1/28167
VL - 44
ER -