Imperial College London


Faculty of MedicineDepartment of Infectious Disease

Senior Research Fellow



+44 (0)20 7594 1803j.bosse




234Wright Fleming WingSt Mary's Campus






BibTex format

author = {Howell, KJ and Weinert, LA and Peters, SE and Wang, J and Hernandez-Garcia, J and Chaudhuri, RR and Luan, S-L and Angen, O and Aragon, V and Williamson, SM and Langford, PR and Rycroft, AN and Wren, BW and Maskell, DJ and Tucker, AW},
doi = {10.1128/JCM.02464-16},
journal = {Journal of Clinical Microbiology},
pages = {2617--2628},
title = {"Pathotyping" Multiplex PCR Assay for haemophilus parasuis: a tool for prediction of virulence},
url = {},
volume = {55},
year = {2017}

RIS format (EndNote, RefMan)

AB - Haemophilus parasuis is a diverse bacterial species that is found in the upper respiratory tracts of pigs and can also cause Glässer's disease and pneumonia. A previous pangenome study of H. parasuis identified 48 genes that were associated with clinical disease. Here, we describe the development of a generalized linear model (termed a pathotyping model) to predict the potential virulence of isolates of H. parasuis based on a subset of 10 genes from the pangenome. A multiplex PCR (mPCR) was constructed based on these genes, the results of which were entered into the pathotyping model to yield a prediction of virulence. This new diagnostic mPCR was tested on 143 field isolates of H. parasuis that had previously been whole-genome sequenced and a further 84 isolates from the United Kingdom from cases of H. parasuis-related disease in pigs collected between 2013 and 2014. The combination of the mPCR and the pathotyping model predicted the virulence of an isolate with 78% accuracy for the original isolate collection and 90% for the additional isolate collection, providing an overall accuracy of 83% (81% sensitivity and 93% specificity) compared with that of the “current standard” of detailed clinical metadata. This new pathotyping assay has the potential to aid surveillance and disease control in addition to serotyping data.
AU - Howell,KJ
AU - Weinert,LA
AU - Peters,SE
AU - Wang,J
AU - Hernandez-Garcia,J
AU - Chaudhuri,RR
AU - Luan,S-L
AU - Angen,O
AU - Aragon,V
AU - Williamson,SM
AU - Langford,PR
AU - Rycroft,AN
AU - Wren,BW
AU - Maskell,DJ
AU - Tucker,AW
DO - 10.1128/JCM.02464-16
EP - 2628
PY - 2017///
SN - 0095-1137
SP - 2617
TI - "Pathotyping" Multiplex PCR Assay for haemophilus parasuis: a tool for prediction of virulence
T2 - Journal of Clinical Microbiology
UR -
UR -
UR -
VL - 55
ER -