Imperial College London

DrJanineBosse

Faculty of MedicineDepartment of Infectious Disease

Senior Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 1803j.bosse

 
 
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Location

 

234Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Bosse:2018:10.1016/j.vetmic.2018.02.019,
author = {Bosse, JT and Li, Y and Sárközi, R and Fodor, L and Lacoutre, S and Gottschalk, M and Amoribieta, MC and Angen, Ø and Nedbalcova, K and Holden, MTG and Maskell, DJ and Tucker, AW and Wren, BW and Rycroft, AN and Langford, PR},
doi = {10.1016/j.vetmic.2018.02.019},
journal = {Veterinary Microbiology},
pages = {1--6},
title = {Proposal of serovars 17 and 18 of Actinobacillus pleuropneumoniae based on serological and genotypic analysis},
url = {http://dx.doi.org/10.1016/j.vetmic.2018.02.019},
volume = {217},
year = {2018}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - The aim of this study was to investigate isolates of Actinobacillus pleuropneumoniae previously designated serologically either as NT or as ‘K2:07’, which did not produce serovar-specific amplicons in PCR assays.We used whole genome sequencing to identify the capsule (CPS) loci of six previously designated biovar 1 non-typable (NT) and two biovar 1 ‘K2:O7’ isolates of A. pleuropneumoniae from Denmark, as well as a recent biovar 2 NT isolate from Canada. All of the NT isolates have the same six-gene type I CPS locus, sharing common cpsABC genes with serovars 2, 3, 6, 7, 8, 9, 11 and 13. The two ‘K2:O7’ isolates contain a unique three-gene type II CPS locus, having a cpsA gene similar to that of serovars 1, 4, 12, 14 and 15. The previously NT isolates share the same O-antigen genes, found between erpA and rpsU, as serovars 3, 6, 8, and 15. Whereas the ‘K2:O7’ isolates, have the same O-antigen genes as serovar 7, which likely contributed to their previous mis-identification. All of the NT and ‘K2:O7’ isolates have only the genes required for production of ApxII (apxIICA structural genes, and apxIBD export genes).Rabbit polyclonal antisera raised against representative isolates with these new CPS loci demonstrated distinct reactivity compared to the 16 known serovars. The serological and genomic results indicate that the isolates constitute new serovars 17 (previously NT) and 18 (previously ‘K2:O7’). Primers designed for amplification of specific serovar 17 and 18 sequences for molecular diagnostics will facilitate epidemiological tracking of these two new serovars of A. pleuropneumoniae.
AU - Bosse,JT
AU - Li,Y
AU - Sárközi,R
AU - Fodor,L
AU - Lacoutre,S
AU - Gottschalk,M
AU - Amoribieta,MC
AU - Angen,Ø
AU - Nedbalcova,K
AU - Holden,MTG
AU - Maskell,DJ
AU - Tucker,AW
AU - Wren,BW
AU - Rycroft,AN
AU - Langford,PR
DO - 10.1016/j.vetmic.2018.02.019
EP - 6
PY - 2018///
SN - 0378-1135
SP - 1
TI - Proposal of serovars 17 and 18 of Actinobacillus pleuropneumoniae based on serological and genotypic analysis
T2 - Veterinary Microbiology
UR - http://dx.doi.org/10.1016/j.vetmic.2018.02.019
UR - http://hdl.handle.net/10044/1/57266
VL - 217
ER -