Imperial College London
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DrJanineBosse

Faculty of Medicine, Department of Infectious Disease

Honorary Senior Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 1803j.bosse

 
 
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Location

 

234Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Li:2018:10.1080/21505594.2018.1491255,
author = {Li, Y and Spiropoulos, J and Cooley, J and Khara, J and Gladstone, C and Asai, M and Bosse, J and Robertson, B and Newton, SM and Langford, P},
doi = {10.1080/21505594.2018.1491255},
journal = {Virulence},
pages = {1126--1137},
title = {Galleria mellonella - a novel infection model for the Mycobacterium tuberculosis complex},
url = {http://dx.doi.org/10.1080/21505594.2018.1491255},
volume = {9},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Animal  models  have  long  been  used  in  tuberculosis  research  to  understand  disease  pathogenesis  and  to  evaluate  novel  vaccine  candidates  and  anti-mycobacterial  drugs.  However,  all  have  limitations  and  there  is  no  single  animal  model  which  mimics  all  the  aspects  of  mycobacterial  pathogenesis  seen  in  humans.  Importantly  mice,  the  most  commonly  used  model,  do  not  normally  form  granulomas,  the  hallmark  of  tuberculosis  infection. Thus there is an urgent need for the development of new alternative in vivo models. The insect larvae, Galleria mellonella has been increasingly used as a successful, simple, widely available and cost-effective model to study microbial infections. Here we report for the first  time  that G.  mellonella  can  be  used  as  an  infection  model  for  members  of  the  M. tuberculosis complex. We demonstrate a dose-response for G. mellonella survival infected with  different  inocula  of  bioluminescent,  Mycobacterium  bovis  BCG  lux, and  demonstrate  suppression  of  mycobacterial  luminesence  over  14  days.  Histopathology  staining  and  transmission  electron  microscopy  of  infected  G.  mellonella  phagocytic  haemocytes  show  internalization  and  aggregation  of  M.  bovis  BCG lux in  granuloma-like  structures,  and  increasing accumulation of lipid bodies within M. bovis BCG lux over time, characteristic of latent  tuberculosis  infection.  Our  results  demonstrate  that  G.  mellonella  can  act  as  a  surrogate host to study the pathogenesis of mycobacterial infection and shed light on host-mycobacteria interactions, including latent tuberculosis infection
AU  - Li,Y
AU  - Spiropoulos,J
AU  - Cooley,J
AU  - Khara,J
AU  - Gladstone,C
AU  - Asai,M
AU  - Bosse,J
AU  - Robertson,B
AU  - Newton,SM
AU  - Langford,P
DO  - 10.1080/21505594.2018.1491255
EP  - 1137
PY  - 2018///
SN  - 2150-5594
SP  - 1126
TI  - Galleria mellonella - a novel infection model for the Mycobacterium tuberculosis complex
T2  - Virulence
UR  - http://dx.doi.org/10.1080/21505594.2018.1491255
UR  - http://hdl.handle.net/10044/1/61321
VL  - 9
ER  -
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