Imperial College London
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DrJanineBosse

Faculty of Medicine, Department of Infectious Disease

Honorary Senior Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 1803j.bosse

 
 
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Location

 

234Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Li:2018:10.3389/fmicb.2018.02489,
author = {Li, Y and da, Silva GC and Li, Y and Rossi, C and Fernandez, Crespo R and Williamson, S and Langford, P and Bazzolli, D and Bosse, J},
doi = {10.3389/fmicb.2018.02489},
journal = {Frontiers in Microbiology},
title = {Evidence of illegitimate recombination between two Pasteurellaceae plasmids resulting in a novel multi-resistance replicon, pM3362MDR, in Actinobacillus pleuropneumoniae},
url = {http://dx.doi.org/10.3389/fmicb.2018.02489},
volume = {9},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Evidence of plasmids carrying the tetracycline resistance gene, tet(B), was found in the previously reported whole genome sequences of 14 United Kingdom, and 4 Brazilian, isolates of Actinobacillus pleuropneumoniae. Isolation and sequencing of selected plasmids, combined with comparative sequence analysis, indicated that the four Brazilian isolates all harbor plasmids that are nearly identical to pB1001, a plasmid previously found in Pasteurella multocida isolates from Spain. Of the United Kingdom isolates, 13/14 harbor plasmids that are (almost) identical to pTetHS016 from Haemophilus parasuis. The remaining United Kingdom isolate, MIDG3362, harbors a 12666 bp plasmid that shares extensive regions of similarity with pOV from P. multocida (which carries blaROB-1, sul2, and strAB genes), as well as with pTetHS016. The newly identified multi-resistance plasmid, pM3362MDR, appears to have arisen through illegitimate recombination of pTetHS016 into the stop codon of the truncated strB gene in a pOV-like plasmid. All of the tet(B)-carrying plasmids studied were capable of replicating in Escherichia coli, and predicted origins of replication were identified. A putative origin of transfer (oriT) sequence with similar secondary structure and a nic-site almost identical to that of RP4 was also identified in these plasmids, however, attempts to mobilize them from an RP4-encoding E. coli donor strain were not successful, indicating that specific conjugation machinery may be required.
AU  - Li,Y
AU  - da,Silva GC
AU  - Li,Y
AU  - Rossi,C
AU  - Fernandez,Crespo R
AU  - Williamson,S
AU  - Langford,P
AU  - Bazzolli,D
AU  - Bosse,J
DO  - 10.3389/fmicb.2018.02489
PY  - 2018///
SN  - 1664-302X
TI  - Evidence of illegitimate recombination between two Pasteurellaceae plasmids resulting in a novel multi-resistance replicon, pM3362MDR, in Actinobacillus pleuropneumoniae
T2  - Frontiers in Microbiology
UR  - http://dx.doi.org/10.3389/fmicb.2018.02489
UR  - http://hdl.handle.net/10044/1/65164
VL  - 9
ER  -
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