Imperial College London
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DrJanineBosse

Faculty of Medicine, Department of Infectious Disease

Honorary Senior Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 1803j.bosse

 
 
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Location

 

234Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Bossé:2010:10.1128/JB.01513-09,
author = {Bossé, JC and Sinha, S and Li, MS and O'Dwyer, CA and Nash, JH and Rycroft, AN and Kroll, JS and Langford, PR},
doi = {10.1128/JB.01513-09},
journal = {Journal of Bacteriology},
pages = {2414--2423},
title = {Regulation of pga operon expression and biofilm formation in Actinobacillus pleuropneumoniae by sigmaE and H-NS.},
url = {http://dx.doi.org/10.1128/JB.01513-09},
volume = {192},
year = {2010}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Clinical isolates of the porcine pathogen Actinobacillus pleuropneumoniae often form adherent colonies on agar plates due to expression of an operon, pgaABCD, encoding a poly-beta-1,6-N-acetyl-D-glucosamine (PGA) extracellular matrix. The adherent colony phenotype, which correlates with the ability to form biofilms on the surfaces of polystyrene plates, is lost following serial passage in broth culture, and repeated passage of the nonadherent variants on solid media does not result in reversion to the adherent colony phenotype. In order to investigate the regulation of PGA expression and biofilm formation in A. pleuropneumoniae, we screened a bank of transposon mutants of the nonadherent serovar 1 strain S4074(T) and identified mutations in two genes, rseA and hns, which resulted in the formation of the adherent colony phenotype. In other bacteria, including the Enterobacteriaceae, H-NS acts as a global gene regulator, and RseA is a negative regulator of the extracytoplasmic stress response sigma factor sigma(E). Transcription profiling of A. pleuropneumoniae rseA and hns mutants revealed that both sigma(E) and H-NS independently regulate expression of the pga operon. Transcription of the pga operon is initiated from a sigma(E) promoter site in the absence of H-NS, and upregulation of sigma(E) is sufficient to displace H-NS, allowing transcription to proceed. In A. pleuropneumoniae, H-NS does not act as a global gene regulator but rather specifically regulates biofilm formation via repression of the pga operon. Positive regulation of the pga operon by sigma(E) indicates that biofilm formation is part of the extracytoplasmic stress response in A. pleuropneumoniae.
AU  - Bossé,JC
AU  - Sinha,S
AU  - Li,MS
AU  - O'Dwyer,CA
AU  - Nash,JH
AU  - Rycroft,AN
AU  - Kroll,JS
AU  - Langford,PR
DO  - 10.1128/JB.01513-09
EP  - 2423
PY  - 2010///
SP  - 2414
TI  - Regulation of pga operon expression and biofilm formation in Actinobacillus pleuropneumoniae by sigmaE and H-NS.
T2  - Journal of Bacteriology
UR  - http://dx.doi.org/10.1128/JB.01513-09
UR  - http://www.ncbi.nlm.nih.gov/pubmed/20207760
UR  - http://jb.asm.org/cgi/content/abstract/192/9/2414
VL  - 192
ER  -
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