Imperial College London
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DrJanineBosse

Faculty of Medicine, Department of Infectious Disease

Honorary Senior Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 1803j.bosse

 
 
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Location

 

234Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Pereira:2015:10.1099/mic.0.083923-0,
author = {Pereira, MF and Rossi, CC and de, Queiroz MV and Martins, GF and Isaac, C and Bosse, JT and Li, Y and Wren, BW and Terra, VS and Cuccui, J and Langford, PR and Soares, Bazzolli DM},
doi = {10.1099/mic.0.083923-0},
journal = {Microbiology},
pages = {387--400},
title = {Galleria mellonella is an effective model to study Actinobacillus pleuropneumoniae infection},
url = {http://dx.doi.org/10.1099/mic.0.083923-0},
volume = {161},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Actinobacillus pleuropneumoniae is responsible for swine pleuropneumonia, a respiratory disease that causes significant global economic loss. Its virulence depends on many factors, such as capsular polysaccharides, RTX toxins and iron-acquisition systems. Analysis of virulence may require easy-to-use models that approximate mammalian infection and avoid ethical issues. Here, we investigate the potential use of the wax moth Galleria mellonella as an informative model for A. pleuropneumoniae infection. Genotypically distinct A. pleuropneumoniae clinical isolates were able to kill larvae at 37 °C but had different LD50 values, ranging from 104 to 107 c.f.u. per larva. The most virulent isolate (1022) was able to persist and replicate within the insect, while the least virulent (780) was rapidly cleared. We observed a decrease in haemocyte concentration, aggregation and DNA damage post-infection with isolate 1022. Melanization points around bacterial cells were observed in the fat body and pericardial tissues of infected G. mellonella, indicating vigorous cell and humoral immune responses close to the larval dorsal vessel. As found in pigs, an A. pleuropneumoniae hfq mutant was significantly attenuated for infection in the G. mellonella model. Additionally, the model could be used to assess the effectiveness of several antimicrobial agents against A. pleuropneumoniae in vivo. G. mellonella is a suitable inexpensive alternative infection model that can be used to study the virulence of A. pleuropneumoniae, as well as assess the effectiveness of antimicrobial agents against this pathogen.
AU  - Pereira,MF
AU  - Rossi,CC
AU  - de,Queiroz MV
AU  - Martins,GF
AU  - Isaac,C
AU  - Bosse,JT
AU  - Li,Y
AU  - Wren,BW
AU  - Terra,VS
AU  - Cuccui,J
AU  - Langford,PR
AU  - Soares,Bazzolli DM
DO  - 10.1099/mic.0.083923-0
EP  - 400
PY  - 2015///
SN  - 1350-0872
SP  - 387
TI  - Galleria mellonella is an effective model to study Actinobacillus pleuropneumoniae infection
T2  - Microbiology
UR  - http://dx.doi.org/10.1099/mic.0.083923-0
VL  - 161
ER  -
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