Imperial College London
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DrJanineBosse

Faculty of Medicine, Department of Infectious Disease

Honorary Senior Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 1803j.bosse

 
 
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Location

 

234Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Cunha:2017:10.1016/j.vetmic.2017.04.019,
author = {Cunha, da silva G and Rossi, CC and Santana, MF and Langford, PR and Bosse, JT and Bazzolli, DM},
doi = {10.1016/j.vetmic.2017.04.019},
journal = {Veterinary Microbiology},
pages = {129--132},
title = {p518, a small floR plasmid from a South American isolate of Actinobacillus pleuropneumoniae},
url = {http://dx.doi.org/10.1016/j.vetmic.2017.04.019},
volume = {204},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - A small (3.9 kb) plasmid (p518), conferring resistance to florfenicol (MIC >8 μg/mL) and chloramphenicol (MIC >8 μg/mL) was isolated from an Actinobacillus pleuropneumoniae clinical isolate from Southeastern Brazil. To date, this is the smallest florfenicol resistance plasmid isolated from a member of the Pasteurellaceae. The complete nucleotide of this plasmid revealed a unique gene arrangement compared to previously reported florfenicol resistance plasmids found in other members of the Pasteurellaceae. In addition to the floR gene and a lysR gene, common to various florfenicol resistance plasmids, p518 also encodes strA and a partial strB sequence. An origin of replication (oriV) similar to that in the broad host range plasmid, pLS88, was identified in p518, and transformation into Escherichia coli MFDpir confirmed the ability to replicate in other species. Mobilisation genes appear to have been lost, with only a partial mobC sequence remaining, and attempts to transfer p518 from a conjugal donor strain (E. coli MFDpir) were not successful, suggesting this plasmid is not mobilisable. Similarly, attempts to transfer p518 into a competent A. pleuropneumoniae strain, MIDG2331, by natural transformation were also not successful. These results suggest that p518 may be only transferred by vertical descent.
AU  - Cunha,da silva G
AU  - Rossi,CC
AU  - Santana,MF
AU  - Langford,PR
AU  - Bosse,JT
AU  - Bazzolli,DM
DO  - 10.1016/j.vetmic.2017.04.019
EP  - 132
PY  - 2017///
SN  - 1873-2542
SP  - 129
TI  - p518, a small floR plasmid from a South American isolate of Actinobacillus pleuropneumoniae
T2  - Veterinary Microbiology
UR  - http://dx.doi.org/10.1016/j.vetmic.2017.04.019
UR  - http://hdl.handle.net/10044/1/48260
VL  - 204
ER  -
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