Imperial College London
Alternate

ProfessorJaneDavies

Faculty of Medicine, National Heart & Lung Institute

Professor of Paediatric Respirology & Experimental Medicine
 
 
 
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Contact

 

+44 (0)20 7594 7973j.c.davies

 
 
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Assistant

 

Mrs Gina Rivellini +44 (0)20 7594 7986

 
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Location

 

G44Emmanuel Kaye BuildingRoyal Brompton Campus

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Summary

 

Publications

Citation

BibTex format

@article{Turnbull:2020:10.1016/j.jcf.2019.05.017,
author = {Turnbull, A and Pyle, C and Patel, D and Jackson, P and Hilliard, T and Regamey, N and Tan, H-L and Brown, S and Thursfield, R and Short, C and Mc, Fie M and Alton, E and Gaggar, A and Blalock, JE and Lloyd, C and Bush, A and Davies, J and Snelgrove, R},
doi = {10.1016/j.jcf.2019.05.017},
journal = {Journal of Cystic Fibrosis},
pages = {40--48},
title = {Abnormal pro-gly-pro pathway and airway neutrophilia in pediatric cystic fibrosis},
url = {http://dx.doi.org/10.1016/j.jcf.2019.05.017},
volume = {19},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BackgroundProline–glycine–proline (PGP) is a bioactive fragment of collagen generated by the action of matrix metalloproteinase-9 (MMP-9) and prolylendopeptidase (PE), and capable of eliciting neutrophil chemotaxis and epithelial remodelling. PGP is normally then degraded by leukotriene A4 hydrolase (LTA4H) to limit inflammation and remodelling. This study hypothesized that early and persistent airway neutrophilia in Cystic Fibrosis (CF) may relate to abnormalities in the PGP pathway and sought to understand underlying mechanisms.MethodsBroncho-alveolar lavage (BAL) fluid was obtained from 38 CF (9 newborns and 29 older children) and 24 non-CF children. BAL cell differentials and levels of PGP, MMP-9, PE and LTA4H were assessed.ResultsWhilst PGP was present in all but one of the older CF children tested, it was absent in non-CF controls and the vast majority of CF newborns. BAL levels of MMP-9 and PE were elevated in older children with CF relative to CF newborns and non-CF controls, correlating with airway neutrophilia and supportive of PGP generation. Furthermore, despite extracellular LTA4H commonly being greatly elevated concomitantly with inflammation to promote PGP degradation, this was not the case in CF children, potentially owing to degradation by neutrophil elastase.ConclusionsA striking imbalance between PGP-generating and -degrading enzymes enables PGP accumulation in CF children from early life and potentially supports airway neutrophilia.
AU  - Turnbull,A
AU  - Pyle,C
AU  - Patel,D
AU  - Jackson,P
AU  - Hilliard,T
AU  - Regamey,N
AU  - Tan,H-L
AU  - Brown,S
AU  - Thursfield,R
AU  - Short,C
AU  - Mc,Fie M
AU  - Alton,E
AU  - Gaggar,A
AU  - Blalock,JE
AU  - Lloyd,C
AU  - Bush,A
AU  - Davies,J
AU  - Snelgrove,R
DO  - 10.1016/j.jcf.2019.05.017
EP  - 48
PY  - 2020///
SN  - 1569-1993
SP  - 40
TI  - Abnormal pro-gly-pro pathway and airway neutrophilia in pediatric cystic fibrosis
T2  - Journal of Cystic Fibrosis
UR  - http://dx.doi.org/10.1016/j.jcf.2019.05.017
UR  - https://www.sciencedirect.com/science/article/pii/S1569199319307696?via%3Dihub
UR  - http://hdl.handle.net/10044/1/70674
VL  - 19
ER  -
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