Imperial College London

DrJamesGardiner

Faculty of MedicineDepartment of Medicine

Reader in Molecular Physiology
 
 
 
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Contact

 

+44 (0)20 3313 3942j.gardiner

 
 
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Location

 

commonwealth building 6S8Commonwealth BuildingHammersmith Campus

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Summary

 

Publications

Publication Type
Year
to

67 results found

Cegla J, Jones BJ, Gardiner JV, Hodson DJ, Marjot T, McGlone ER, Tan TM, Bloom SRet al., 2017, RAMP2 Influences Glucagon Receptor Pharmacology via Trafficking and Signaling, 52nd Annual Meeting of the European-Association-for-the-Study-of-Diabetes (EASD), Publisher: OXFORD UNIV PRESS INC, Pages: 2680-2693, ISSN: 0013-7227

CONFERENCE PAPER

Cork SC, Eftekhar A, Mirza KB, Gardiner JV, Bloom SR, Toumazou Cet al., 2017, Extracellular pH monitoring for use in closed-loop vagus nerve stimulation., J Neural Eng

OBJECTIVE: Vagal nerve stimulation (VNS) has shown potential benefits for obesity treatment; however current devices lack physiological feedback, which limit their efficacy. Changes in extracellular pH (pH<sub>e</sub>) have shown to be correlated with neural activity, but have traditionally been measured with glass microelectrodes, which limit their in vivo applicability. APPROACH: Iridium oxide has previously been shown to be sensitive to fluctuations in pH and is biocompatible. Iridium oxide microelectrodes were inserted into the subdiaphragmatic vagus nerve of anaesthetised rats. Introduction of the gut hormone cholecystokinin (CCK) or distension of the stomach was used to elicit vagal nerve activity. MAIN RESULTS: iridium oxide microelectrodes have sufficient pH sensitivity to readily detect changes in pH<sub>e</sub> associated with both CCK and gastric distension. What's more, custom made Matlab script was able to use these changes in pH<sub>e</sub> to automatically trigger an implanted VNS device. SIGNIFICANCE: This is the first study to show pH<sub>e</sub> changes in peripheral nerves in vivo. Furthermore, the demonstration that iridium oxide microelectrodes are sufficiently pH sensitive as to measure changes in pH<sub>e</sub> associated with physiological stimuli means they have the potential to be integrated into closed-loop neurostimulating devices.

JOURNAL ARTICLE

Hameed S, Patterson M, Dhillo WS, Rahman SA, Ma Y, Holton C, Gogakos A, Yeo GSH, Lam BYH, Polex-Wolf J, Fenske W, Bell J, Anastasovska J, Samarut J, Bloom SR, Bassett JHD, Williams GR, Gardiner JVet al., 2017, Thyroid Hormone Receptor Beta in the Ventromedial Hypothalamus Is Essential for the Physiological Regulation of Food Intake and Body Weight, CELL REPORTS, Vol: 19, Pages: 2202-2209, ISSN: 2211-1247

JOURNAL ARTICLE

Ma Y, Ratnasabapathy R, Gardiner J, 2017, Carbohydrate craving: not everything is sweet, CURRENT OPINION IN CLINICAL NUTRITION AND METABOLIC CARE, Vol: 20, Pages: 261-265, ISSN: 1363-1950

JOURNAL ARTICLE

De Backer I, Hussain SS, Bloom SR, Gardiner JVet al., 2016, Insights into the role of neuronal glucokinase, AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, Vol: 311, Pages: E42-E55, ISSN: 0193-1849

JOURNAL ARTICLE

Hu MH, Li XF, McCausland B, Li SY, Gresham R, Kinsey-Jones JS, Gardiner JV, Sam AH, Bloom SR, Poston L, Lightman SL, Murphy KG, O'Byrne KTet al., 2015, Relative Importance of the Arcuate and Anteroventral Periventricular Kisspeptin Neurons in Control of Puberty and Reproductive Function in Female Rats, ENDOCRINOLOGY, Vol: 156, Pages: 2619-2631, ISSN: 0013-7227

JOURNAL ARTICLE

Hussain S, Richardson E, Ma Y, Holton C, De Backer I, Buckley N, Dhillo W, Bewick G, Zhang S, Carling D, Bloom S, Gardiner Jet al., 2015, Glucokinase activity in the arcuate nucleus regulates glucose intake., J Clin Invest, Vol: 125, Pages: 337-349

The brain relies on a constant supply of glucose, its primary fuel, for optimal function. A taste-independent mechanism within the CNS that promotes glucose delivery to the brain has been postulated to maintain glucose homeostasis; however, evidence for such a mechanism is lacking. Here, we determined that glucokinase activity within the hypothalamic arcuate nucleus is involved in regulation of dietary glucose intake. In fasted rats, glucokinase activity was specifically increased in the arcuate nucleus but not other regions of the hypothalamus. Moreover, pharmacologic and genetic activation of glucokinase in the arcuate nucleus of rodent models increased glucose ingestion, while decreased arcuate nucleus glucokinase activity reduced glucose intake. Pharmacologic targeting of potential downstream glucokinase effectors revealed that ATP-sensitive potassium channel and P/Q calcium channel activity are required for glucokinase-mediated glucose intake. Additionally, altered glucokinase activity affected release of the orexigenic neurotransmitter neuropeptide Y in response to glucose. Together, our results suggest that glucokinase activity in the arcuate nucleus specifically regulates glucose intake and that appetite for glucose is an important driver of overall food intake. Arcuate nucleus glucokinase activation may represent a CNS mechanism that underlies the oft-described phenomena of the "sweet tooth" and carbohydrate craving.

JOURNAL ARTICLE

Ma Y, Bloom SR, Gardiner JV, 2015, Arcuate nucleus glucokinase and dietary glucose intake, ONCOTARGET, Vol: 6, Pages: 19926-19927, ISSN: 1949-2553

JOURNAL ARTICLE

McGavigan AK, O'Hara HC, Amin A, Kinsey-Jones J, Spreckley E, Alamshah A, Agahi A, Banks K, France R, Hyberg G, Wong C, Bewick GA, Gardiner JV, Lehmann A, Martin NM, Ghatei MA, Bloom SR, Murphy KGet al., 2015, L-cysteine suppresses ghrelin and reduces appetite in rodents and humans, INTERNATIONAL JOURNAL OF OBESITY, Vol: 39, Pages: 447-455, ISSN: 0307-0565

JOURNAL ARTICLE

Beale KE, Kinsey-Jones JS, Gardiner JV, Harrison EK, Thompson EL, Hu MH, Sleeth ML, Sam AH, Greenwood HC, McGavigan AK, Dhillo WS, Mora JM, Li XF, Franks S, Bloom SR, O'Byrne KT, Murphy KGet al., 2014, The Physiological Role of Arcuate Kisspeptin Neurons in the Control of Reproductive Function in Female Rats, ENDOCRINOLOGY, Vol: 155, Pages: 1091-1098, ISSN: 0013-7227

JOURNAL ARTICLE

McGowan BM, Minnion JS, Murphy KG, Roy D, Stanley SA, Dhillo WS, Gardiner JV, Ghatei MA, Bloom SRet al., 2014, Relaxin-3 stimulates the neuro-endocrine stress axis via corticotrophin-releasing hormone, JOURNAL OF ENDOCRINOLOGY, Vol: 221, Pages: 337-346, ISSN: 0022-0795

JOURNAL ARTICLE

Beale KEL, Gardiner JV, Bewick GA, Hostomska K, Patel NA, Hussain SS, Jayasena CN, Ebling FJP, Jethwa PH, Prosser HM, Lattanzi R, Negri L, Ghatei MA, Bloom SR, Dhillo WSet al., 2013, Peripheral administration of prokineticin 2 potently reduces food intake and body weight in mice via the brainstem, BRITISH JOURNAL OF PHARMACOLOGY, Vol: 168, Pages: 403-410, ISSN: 0007-1188

JOURNAL ARTICLE

Patterson M, Bloom SR, Gardiner JV, 2011, Ghrelin and appetite control in humans-Potential application in the treatment of obesity, PEPTIDES, Vol: 32, Pages: 2290-2294, ISSN: 0196-9781

JOURNAL ARTICLE

Boughton CK, Patterson M, Bewick GA, Tadross JA, Gardiner JV, Beale KEL, Chaudery F, Hunter G, Busbridge M, Leavy EM, Ghatei MA, Bloom SR, Murphy KGet al., 2010, Alarin stimulates food intake and gonadotrophin release in male rats, BRITISH JOURNAL OF PHARMACOLOGY, Vol: 161, Pages: 601-613, ISSN: 0007-1188

JOURNAL ARTICLE

Boughton CK, Patterson M, Tadross JA, Bewick GA, Gardiner JV, Ghatei MA, Bloom SR, Murphy KGet al., 2010, The Novel Neuropeptide Alarin Stimulates Food Intake and the Reproductive Axis in Male Rats., 92nd Meeting and Expo of the Endocrine Society (ENDO 2010), Publisher: ENDOCRINE SOC, ISSN: 0163-769X

CONFERENCE PAPER

Gardiner JV, Bataveljic A, Patel NA, Bewick GA, Roy D, Campbell D, Greenwood HC, Murphy KG, Hameed S, Jethwa PH, Ebling FJP, Vickers SP, Cheetham S, Ghatei MA, Bloom SR, Dhillo WSet al., 2010, Prokineticin 2 Is a Hypothalamic Neuropeptide That Potently Inhibits Food Intake, DIABETES, Vol: 59, Pages: 397-406, ISSN: 0012-1797

JOURNAL ARTICLE

Gardiner JV, Beale KE, Roy D, Boughton CK, Bataveljic A, Campbell DC, Bewick GA, Patel NA, Patterson M, Leavy EM, Ghatei MA, Bloom SR, Dhillo WSet al., 2010, Cerebellin1 is a novel orexigenic peptide, DIABETES OBESITY & METABOLISM, Vol: 12, Pages: 883-890, ISSN: 1462-8902

JOURNAL ARTICLE

Gardiner JV, Campbell D, Patterson M, Kent A, Ghatei MA, Bloom SR, Bewick GAet al., 2010, The Hyperphagic Effect of Ghrelin Is Inhibited in Mice by a Diet High in Fat, GASTROENTEROLOGY, Vol: 138, Pages: 2468-U338, ISSN: 0016-5085

JOURNAL ARTICLE

Leavy EM, Gardiner JV, Richardson E, Buckley NW, Martin NM, Bloom SR, Smith KLet al., 2010, The Role of Neuropeptide Y within the Hypothalamic Dorsomedial Nucleus in the Regulation of Energy Homeostasis., 92nd Meeting and Expo of the Endocrine Society (ENDO 2010), Publisher: ENDOCRINE SOC, ISSN: 0163-769X

CONFERENCE PAPER

McGowan BMC, Minnion JS, Murphy KG, White NE, Roy D, Stanley SA, Dhillo WS, Gardiner JV, Ghatei MA, Bloom SRet al., 2010, Central and peripheral administration of human relaxin-2 to adult male rats inhibits food intake, DIABETES OBESITY & METABOLISM, Vol: 12, Pages: 1090-1096, ISSN: 1462-8902

JOURNAL ARTICLE

Richardson E, Hussain SS, Counsell JR, Leavy E, Bewick GA, Bloom SR, Gardiner JVet al., 2010, The Role of Ventromedial Hypothalamic Glucose-Sensing Neurons during Glucoprivation., 92nd Meeting and Expo of the Endocrine Society (ENDO 2010), Publisher: ENDOCRINE SOC, ISSN: 0163-769X

CONFERENCE PAPER

Bewick GA, Kent A, Campbell D, Patterson M, Ghatei MA, Bloom SR, Gardiner JVet al., 2009, Mice With Hyperghrelinemia Are Hyperphagic and Glucose Intolerant and Have Reduced Leptin Sensitivity, DIABETES, Vol: 58, Pages: 840-846, ISSN: 0012-1797

JOURNAL ARTICLE

Dhillo WS, Bewick GA, White NE, Gardiner JV, Thompson EL, Bataveljic A, Murphy KG, Roy D, Patel NA, Scutt JN, Armstrong A, Ghatei MA, Bloom SRet al., 2009, The thyroid hormone derivative 3-iodothyronamine increases food intake in rodents, DIABETES OBESITY & METABOLISM, Vol: 11, Pages: 251-260, ISSN: 1462-8902

JOURNAL ARTICLE

Gardiner J, Bloom S, 2008, Ghrelin gets its GOAT, CELL METABOLISM, Vol: 7, Pages: 193-194, ISSN: 1550-4131

JOURNAL ARTICLE

Gardiner JV, Jayasena CN, Bloom SR, 2008, Gut hormones: A weight off your mind, JOURNAL OF NEUROENDOCRINOLOGY, Vol: 20, Pages: 834-841, ISSN: 0953-8194

JOURNAL ARTICLE

McGowan BM, Stanley SA, Donovan J, Thompson EL, Patterson M, Semjonous NM, Gardiner JV, Murphy KG, Ghatei MA, Bloom SRet al., 2008, Relaxin-3 stimulates the hypothalamic-pituitary-gonadal axis, AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, Vol: 295, Pages: E278-E286, ISSN: 0193-1849

JOURNAL ARTICLE

Smith KL, Gardiner JV, Ward HL, Kong WM, Murphy KG, Martin NM, Ghatei MA, Bloom SRet al., 2008, Overexpression of CART in the PVN increases food intake and weight gain in rats, OBESITY, Vol: 16, Pages: 2239-2244, ISSN: 1930-7381

JOURNAL ARTICLE

White NE, Dhillo WS, Liu Y-L, Small CJ, Kennett GA, Gardiner JV, Ghatei MA, Bloom SRet al., 2008, Co-administration of SR141716 with peptide YY3-36 or oxyntomodulin has additive effects on food intake in mice, DIABETES OBESITY & METABOLISM, Vol: 10, Pages: 167-170, ISSN: 1462-8902

JOURNAL ARTICLE

McGowan BM, Stanley SA, White NE, Spangeus A, Patterson M, Thompson EL, Smith KL, Donovan J, Gardiner JV, Ghatei MA, Bloom SRet al., 2007, Hypothalamic mapping of orexigenic action and Fos-like immunoreactivity following relaxin-3 administration in male Wistar rats, AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, Vol: 292, Pages: E913-E919, ISSN: 0193-1849

JOURNAL ARTICLE

McGowan BM, Stanley SA, Smith KL, Minnion JS, Donovan J, Thompson EL, Patterson M, Connolly MM, Abbott CR, Small CJ, Gardiner JV, Ghatel MA, Bloom SRet al., 2006, Effects of acute and chronic relaxin-3 on food intake and energy expenditure in rats, REGULATORY PEPTIDES, Vol: 136, Pages: 72-77, ISSN: 0167-0115

JOURNAL ARTICLE

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