Imperial College London

Krishnan

Faculty of EngineeringDepartment of Chemical Engineering

Senior Lecturer
 
 
 
//

Contact

 

+44 (0)20 7594 6633j.krishnan

 
 
//

Location

 

C503Roderic Hill BuildingSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Gorgoni:2019:10.3390/cells8080800,
author = {Gorgoni, B and Zhao, Y-B and Krishnan, J and Stansfield, I},
doi = {10.3390/cells8080800},
journal = {Cells},
title = {Destabilisation of eukaryote mRNAs by 5’ proximal stop codons can occur independently of the nonsense-mediated mRNA decay pathway},
url = {http://dx.doi.org/10.3390/cells8080800},
volume = {8},
year = {2019}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - In eukaryotes, the binding of poly(A) binding protein (PAB) to the poly(A) tail is central to maintaining mRNA stability. PABP interacts with the translation termination apparatus, and with eIF4G to maintain 3′–5′ mRNA interactions as part of an mRNA closed loop. It is however unclear how ribosome recycling on a closed loop mRNA is influenced by the proximity of the stop codon to the poly(A) tail, and how post-termination ribosome recycling affects mRNA stability. We show that in a yeast disabled for nonsense mediated mRNA decay (NMD), a PGK1 mRNA with an early stop codon at codon 22 of the reading frame is still highly unstable, and that this instability cannot be significantly countered even when 50% stop codon readthrough is triggered. In an NMD-deficient mutant yeast, stable reporter alleles with more 3′ proximal stop codons could not be rendered unstable through Rli1-depletion, inferring defective Rli1 ribosome recycling is insufficient in itself to trigger mRNA instability. Mathematical modelling of a translation system including the effect of ribosome recycling and poly(A) tail shortening supports the hypothesis that impaired ribosome recycling from 5′ proximal stop codons may compromise initiation processes and thus destabilize the mRNA. A model is proposed wherein ribosomes undergo a maturation process during early elongation steps, and acquire competency to re-initiate on the same mRNA as translation elongation progresses beyond the very 5′ proximal regions of the mRNA.
AU - Gorgoni,B
AU - Zhao,Y-B
AU - Krishnan,J
AU - Stansfield,I
DO - 10.3390/cells8080800
PY - 2019///
SN - 2073-4409
TI - Destabilisation of eukaryote mRNAs by 5’ proximal stop codons can occur independently of the nonsense-mediated mRNA decay pathway
T2 - Cells
UR - http://dx.doi.org/10.3390/cells8080800
UR - http://hdl.handle.net/10044/1/72422
VL - 8
ER -