Imperial College London
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DrJohnLees

Faculty of Medicine, School of Public Health

Visiting Researcher
 
 
 
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Contact

 

+44 (0)20 7594 2939j.lees Website

 
 
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Location

 

UG4Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Aggarwal:2022:10.1101/2022.08.05.500807,
author = {Aggarwal, SD and Lees, JA and Jacobs, NT and Bee, GCW and Abruzzo, AR and Weiser, JN},
doi = {10.1101/2022.08.05.500807},
title = {BlpC-mediated selfish program leads to rapid loss of <i>Streptococcus pneumoniae</i> clonal diversity during infection},
url = {http://dx.doi.org/10.1101/2022.08.05.500807},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - <jats:title>SUMMARY</jats:title><jats:p>Chromosomal barcoding and high-throughput sequencing were used to investigate the population dynamics of <jats:italic>Streptococcus pneumoniae</jats:italic>. During infant mouse colonization, >35-fold reduction in diversity and expansion of a single clonal lineage was observed within 1 day post-inoculation. This loss of diversity was not due to immune factors, host microbiota or exclusively because of genetic drift. Rather, it required the expression of <jats:italic>blp</jats:italic> bacteriocins induced by the BlpC-quorum sensing pheromone. This points towards the role of intra-strain competition whereby the subpopulation reaching a quorum eliminates others that have yet to activate the <jats:italic>blp</jats:italic> locus. We show that this loss of diversity also restricts the number of unique clones that could establish colonization during transmission between hosts. Moreover, we show that genetic variation in the <jats:italic>blp</jats:italic> locus is associated with transmissibility in the human population. We posit this is due to its importance in clonal selection and its role as a selfish element.</jats:p>
AU  - Aggarwal,SD
AU  - Lees,JA
AU  - Jacobs,NT
AU  - Bee,GCW
AU  - Abruzzo,AR
AU  - Weiser,JN
DO  - 10.1101/2022.08.05.500807
PY  - 2022///
TI  - BlpC-mediated selfish program leads to rapid loss of <i>Streptococcus pneumoniae</i> clonal diversity during infection
UR  - http://dx.doi.org/10.1101/2022.08.05.500807
ER  -
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