Imperial College London


Faculty of MedicineDepartment of Infectious Disease

Research Fellow



+44 (0)20 7594 3058j.macdonald Website




Sir Alexander Fleming BuildingSouth Kensington Campus





I am a synthetic biologist working on cell-free protein expression systems and computational protein design. I develop computational methods as well as working in the wet-lab.

Please visit my personal web page to learn more about my research:



Freemont PS, Moore S, MacDonald J, et al., 2018, Rapid acquisition and model-based analysis of cell-free transcription-translation reactions from non-model bacteria, Proceedings of the National Academy of Sciences, Vol:115, ISSN:0027-8424, Pages:E4340-E4349

Freemont PS, Salih O, He S, et al., 2018, Atomic Structure of Type VI Contractile Sheath from Pseudomonas aeruginosa, Structure, Vol:26, ISSN:0969-2126, Pages:329-336.e3

MacDonald JT, Siciliano V, 2017, Computational Sequence Design with R2oDNA Designer., Methods Mol Biol, Vol:1651, Pages:249-262

Moore SJ, macdonald JT, freemont PS, 2017, Cell-free synthetic biology for in vitro prototype engineering, Biochemical Society Transactions, Vol:45, ISSN:1470-8752, Pages:785-791

MacDonald J, Freemont PS, 2016, Computational protein design with backbone plasticity, Biochemical Society Transactions, Vol:44, ISSN:1470-8752, Pages:1523-1529

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