Imperial College London

Emeritus ProfessorJohnMansfield

Faculty of Natural SciencesDepartment of Life Sciences

Emeritus Professor of Plant Pathology
 
 
 
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Contact

 

+44 (0)20 7594 5394j.mansfield

 
 
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Location

 

Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

167 results found

Hulin MT, Armitage AD, Vicente JG, Holub EB, Baxter L, Bates HJ, Mansfield JW, Jackson RW, Harrison RJet al., 2018, Comparative genomics of Pseudomonas syringae reveals convergent gene gain and loss associated with specialization onto cherry (Prunus avium)., New Phytol

Genome-wide analyses of the effector- and toxin-encoding genes were used to examine the phylogenetics and evolution of pathogenicity amongst diverse strains of Pseudomonas syringae causing bacterial canker of cherry (Prunus avium), including pathovars P. syringae pv morsprunorum (Psm) races 1 and 2, P. syringae pv syringae (Pss) and P. syringae pv avii. Phylogenetic analyses revealed Psm races and P. syringae pv avii clades were distinct and were each monophyletic, whereas cherry-pathogenic strains of Pss were interspersed amongst strains from other host species. A maximum likelihood approach was used to predict effectors associated with pathogenicity on cherry. Pss possesses a smaller repertoire of type III effectors but has more toxin biosynthesis clusters than Psm and P. syringae pv avii. Evolution of cherry pathogenicity was correlated with gain of genes such as hopAR1 and hopBB1 through putative phage transfer and horizontal transfer respectively. By contrast, loss of the avrPto/hopAB redundant effector group was observed in cherry-pathogenic clades. Ectopic expression of hopAB and hopC1 triggered the hypersensitive reaction in cherry leaves, confirming computational predictions. Cherry canker provides a fascinating example of convergent evolution of pathogenicity that is explained by the mix of effector and toxin repertoires acting on a common host.

JOURNAL ARTICLE

Hulin MT, Mansfield JW, Brain P, Xu X, Jackson RW, Harrison RJet al., 2018, Characterization of the pathogenicity of strains of Pseudomonas syringae towards cherry and plum, Plant Pathology, Vol: 67, Pages: 1177-1193, ISSN: 0032-0862

© 2018 The Authors. Plant Pathology published by John Wiley & Sons Ltd on behalf of British Society for Plant Pathology. Bacterial canker is a major disease of Prunus avium (cherry), Prunus domestica (plum) and other stone fruits. It is caused by pathovars within the Pseudomonas syringae species complex including P. syringae pv. morsprunorum (Psm) race 1 (R1), Psm race 2 (R2) and P. syringae pv. syringae (Pss). Psm R1 and Psm R2 were originally designated as the same pathovar; however, phylogenetic analysis revealed them to be distantly related, falling into phylogroups 3 and 1, respectively. This study characterized the pathogenicity of 18 newly genome-sequenced P. syringae strains on cherry and plum, in the field and laboratory. The field experiment confirmed that the cherry cultivar Merton Glory exhibited a broad resistance to all clades. Psm R1 contained strains with differential specificity on cherry and plum. The ability of tractable laboratory-based assays to reproduce assessments on whole trees was examined. Good correlations were achieved with assays using cut shoots or leaves, although only the cut shoot assay was able to reliably discriminate cultivar differences seen in the field. Measuring bacterial multiplication in detached leaves differentiated pathogens from nonpathogens and was therefore suitable for routine testing. In cherry leaves, symptom appearance discriminated Psm races from nonpathogens, which triggered a hypersensitive reaction. Pathogenic strains of Pss rapidly induced disease lesions in all tissues and exhibited a more necrotrophic lifestyle than hemibiotrophic Psm. This in-depth study of pathogenic interactions, identification of host resistance and optimization of laboratory assays provides a framework for future genetic dissection of host–pathogen interactions in the canker disease.

JOURNAL ARTICLE

Rufian JS, Macho AP, Corry DS, Mansfield JW, Ruiz-Albert J, Arnold DL, Beuzon CRet al., 2018, Confocal microscopy reveals in planta dynamic interactions between pathogenic, avirulent and non-pathogenic Pseudomonas syringae strains, MOLECULAR PLANT PATHOLOGY, Vol: 19, Pages: 537-551, ISSN: 1464-6722

JOURNAL ARTICLE

Rufian JS, Sanchez-Romero M-A, Lopez-Marquez D, Macho AP, Mansfield JW, Arnold DL, Ruiz-Albert J, Casadesus J, Beuzon CRet al., 2016, Pseudomonas syringae differentiates into phenotypically distinct subpopulations during colonization of a plant host, ENVIRONMENTAL MICROBIOLOGY, Vol: 18, Pages: 3593-3605, ISSN: 1462-2912

JOURNAL ARTICLE

Stewart SA, Hodge S, Bennett M, Mansfield JW, Powell Get al., 2016, Aphid induction of phytohormones in Medicago truncatula is dependent upon time post-infestation, aphid density and the genotypes of both plant and insect, ARTHROPOD-PLANT INTERACTIONS, Vol: 10, Pages: 41-53, ISSN: 1872-8855

JOURNAL ARTICLE

Wye with Hinxhill Parish Council, 2016, Wye Neighbourhood Development Plan, Ashford Borough Council

OTHER

Li B, Hulin MT, Brain P, Mansfield JW, Jackson RW, Harrison RJet al., 2015, Rapid, automated detection of stem canker symptoms in woody perennials using artificial neural network analysis, PLANT METHODS, Vol: 11, ISSN: 1746-4811

JOURNAL ARTICLE

Mitchell K, Brown I, Knox P, Mansfield Jet al., 2015, The role of cell wall-based defences in the early restriction of non-pathogenic hrp mutant bacteria in Arabidopsis, PHYTOCHEMISTRY, Vol: 112, Pages: 139-150, ISSN: 0031-9422

JOURNAL ARTICLE

Zabala MDT, Littlejohn G, Jayaraman S, Studholme D, Bailey T, Lawson T, Tillich M, Licht D, Boelter B, Delfino L, Truman W, Mansfield J, Smirnoff N, Grant Met al., 2015, Chloroplasts play a central role in plant defence and are targeted by pathogen effectors, NATURE PLANTS, Vol: 1, ISSN: 2055-026X

JOURNAL ARTICLE

Hodge S, Ward JL, Beale MH, Bennett M, Mansfield JW, Powell Get al., 2013, Aphid-induced accumulation of trehalose in Arabidopsis thaliana is systemic and dependent upon aphid density, PLANTA, Vol: 237, Pages: 1057-1064, ISSN: 0032-0935

JOURNAL ARTICLE

Mansfield J, Genin S, Magori S, Citovsky V, Sriariyanum M, Ronald P, Dow M, Verdier V, Beer SV, Machado MA, Toth I, Salmond G, Foster GDet al., 2012, Top 10 plant pathogenic bacteria in molecular plant pathology, MOLECULAR PLANT PATHOLOGY, Vol: 13, Pages: 614-629, ISSN: 1464-6722

JOURNAL ARTICLE

Pham J, Liu J, Bennett MH, Mansfield JW, Desikan Ret al., 2012, Arabidopsis histidine kinase 5 regulates salt sensitivity and resistance against bacterial and fungal infection, NEW PHYTOLOGIST, Vol: 194, Pages: 168-180, ISSN: 0028-646X

JOURNAL ARTICLE

Arnold DL, Lovell HC, Jackson RW, Mansfield JWet al., 2011, Pseudomonas syringae pv. phaseolicola: from 'has bean' to supermodel, MOLECULAR PLANT PATHOLOGY, Vol: 12, Pages: 617-627, ISSN: 1464-6722

JOURNAL ARTICLE

Denness L, McKenna JF, Segonzac C, Wormit A, Madhou P, Bennett M, Mansfield J, Zipfel C, Hamann Tet al., 2011, Cell Wall Damage-Induced Lignin Biosynthesis Is Regulated by a Reactive Oxygen Species- and Jasmonic Acid-Dependent Process in Arabidopsis, PLANT PHYSIOLOGY, Vol: 156, Pages: 1364-1374, ISSN: 0032-0889

JOURNAL ARTICLE

Godfrey SAC, Lovell HC, Mansfield JW, Corry DS, Jackson RW, Arnold DLet al., 2011, The Stealth Episome: Suppression of Gene Expression on the Excised Genomic Island PPHGI-1 from Pseudomonas syringae pv. phaseolicola, PLOS PATHOGENS, Vol: 7, ISSN: 1553-7374

JOURNAL ARTICLE

Lovell HC, Jackson RW, Mansfield JW, Godfrey SAC, Hancock JT, Desikan R, Arnold DLet al., 2011, In planta conditions induce genomic changes in Pseudomonas syringae pv. phaseolicola, MOLECULAR PLANT PATHOLOGY, Vol: 12, Pages: 167-176, ISSN: 1464-6722

JOURNAL ARTICLE

Forcat S, Bennett M, Grant M, Mansfield JWet al., 2010, Rapid linkage of indole carboxylic acid to the plant cell wall identified as a component of basal defence in Arabidopsis against hrp mutant bacteria, PHYTOCHEMISTRY, Vol: 71, Pages: 870-876, ISSN: 0031-9422

JOURNAL ARTICLE

Forsyth A, Mansfield JW, Grabov N, de Torres M, Sinapidou E, Grant MRet al., 2010, Genetic Dissection of Basal Resistance to Pseudomonas syringae pv. phaseolicola in Accessions of Arabidopsis, MOLECULAR PLANT-MICROBE INTERACTIONS, Vol: 23, Pages: 1545-1552, ISSN: 0894-0282

JOURNAL ARTICLE

Godfrey SAC, Mansfield JW, Corry DS, Lovell HC, Jackson RW, Arnold DLet al., 2010, Confocal Imaging of Pseudomonas syringae pv. phaseolicola Colony Development in Bean Reveals Reduced Multiplication of Strains Containing the Genomic Island PPHGI-1, MOLECULAR PLANT-MICROBE INTERACTIONS, Vol: 23, Pages: 1294-1302, ISSN: 0894-0282

JOURNAL ARTICLE

Mansfield J, 2010, New Honorary Member of BSPP, PLANT PATHOLOGY, Vol: 59, Pages: 1-2, ISSN: 0032-0862

JOURNAL ARTICLE

Ortiz-Martin I, Thwaites R, Macho AP, Mansfield JW, Beuzon CRet al., 2010, Positive Regulation of the Hrp Type III Secretion System in Pseudomonas syringae pv. phaseolicola, MOLECULAR PLANT-MICROBE INTERACTIONS, Vol: 23, Pages: 665-681, ISSN: 0894-0282

JOURNAL ARTICLE

Ortiz-Martin I, Thwaites R, Mansfield JW, Beuzon CRet al., 2010, Negative Regulation of the Hrp Type III Secretion System in Pseudomonas syringae pv. phaseolicola, MOLECULAR PLANT-MICROBE INTERACTIONS, Vol: 23, Pages: 682-701, ISSN: 0894-0282

JOURNAL ARTICLE

Ward JL, Forcat S, Beckmann M, Bennett M, Miller SJ, Baker JM, Hawkins ND, Vermeer CP, Lu C, Lin W, Truman WM, Beale MH, Draper J, Mansfield JW, Grant Met al., 2010, The metabolic transition during disease following infection of Arabidopsis thaliana by Pseudomonas syringae pv. tomato, PLANT JOURNAL, Vol: 63, Pages: 443-457, ISSN: 0960-7412

JOURNAL ARTICLE

Hamann T, Bennett M, Mansfield J, Somerville Cet al., 2009, Identification of cell-wall stress as a hexose-dependent and osmosensitive regulator of plant responses, PLANT JOURNAL, Vol: 57, Pages: 1015-1026, ISSN: 0960-7412

JOURNAL ARTICLE

Lovell HC, Mansfield JW, Godfrey SAC, Jackson RW, Hancock JT, Arnold DLet al., 2009, Bacterial Evolution by Genomic Island Transfer Occurs via DNA Transformation In Planta, CURRENT BIOLOGY, Vol: 19, Pages: 1586-1590, ISSN: 0960-9822

JOURNAL ARTICLE

Mansfield JW, 2009, From bacterial avirulence genes to effector functions via the hrp delivery system: an overview of 25 years of progress in our understanding of plant innate immunity, MOLECULAR PLANT PATHOLOGY, Vol: 10, Pages: 721-734, ISSN: 1464-6722

JOURNAL ARTICLE

Stewart SA, Hodge S, Ismail N, Mansfield JW, Feys BJ, Prosperi J-M, Huguet T, Ben C, Gentzbittel L, Powell Get al., 2009, The RAP1 Gene Confers Effective, Race-Specific Resistance to the Pea Aphid in Medicago truncatula Independent of the Hypersensitive Reaction, MOLECULAR PLANT-MICROBE INTERACTIONS, Vol: 22, Pages: 1645-1655, ISSN: 0894-0282

JOURNAL ARTICLE

Forcat S, Bennett MH, Mansfield JW, Grant MRet al., 2008, A rapid and robust method for simultaneously measuring changes in the phytohormones ABA, JA and SA in plants following biotic and abiotic stress, PLANT METHODS, Vol: 4, ISSN: 1746-4811

JOURNAL ARTICLE

Goehre V, Spallek T, Haeweker H, Mersmann S, Mentzel T, Boller T, de Torres M, Mansfield JW, Robatzek Set al., 2008, Plant Pattern-Recognition Receptor FLS2 Is Directed for Degradation by the Bacterial Ubiquitin Ligase AvrPtoB, CURRENT BIOLOGY, Vol: 18, Pages: 1824-1832, ISSN: 0960-9822

JOURNAL ARTICLE

Tsiamis G, Siozios S, Mansfield J, Bourtzis Ket al., 2008, Expression of the HrpA gene product in planta activates plant immunity in Arabidopsis thaliana, Joint Conference of the 33rd FEBS Congress/11th IUBMB Conference, Publisher: BLACKWELL PUBLISHING, Pages: 276-276, ISSN: 1742-464X

CONFERENCE PAPER

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