Imperial College London
Alternate

Emeritus ProfessorJeremyNicholson

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Emeritus Professor of Biological Chemistry
 
 
 
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Contact

 

+44 (0)20 7594 3195j.nicholson Website

 
 
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Assistant

 

Ms Wendy Torto +44 (0)20 7594 3225

 
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Location

 

Office no. 665Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Letertre:2020:10.1007/s10337-020-03900-4,
author = {Letertre, M and Munjoma, NC and Slade, SE and Plumb, RS and Swann, J and Coen, M and Nicholson, JK and Wilson, ID},
doi = {10.1007/s10337-020-03900-4},
journal = {Chromatographia},
pages = {853--861},
title = {Metabolic phenotyping using UPLC–MS and rapid microbore UPLC–IM–MS: determination of the effect of different dietary regimes on the urinary metabolome of the rat},
url = {http://dx.doi.org/10.1007/s10337-020-03900-4},
volume = {83},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - A rapid reversed-phase gradient method employing a 50 mm × 1 mm i.d., C18 microbore column, combined with ion mobility and high-resolution mass spectrometry, was applied to the metabolic phenotyping of urine samples obtained from rats receiving different diets. This method was directly compared to a “conventional” method employing a 150 × 2.1 mm i.d. column packed with the same C18 bonded phase using the same samples. Multivariate statistical analysis of the resulting data showed similar class discrimination for both microbore and conventional methods, despite the detection of fewer mass/retention time features by the former. Multivariate statistical analysis highlighted a number of ions that represented diet-specific markers in the samples. Several of these were then identified using the combination of mass, ion-mobility-derived collision cross section and retention time including N-acetylglutamate, urocanic acid, and xanthurenic acid. Kynurenic acid was tentatively identified based on mass and ion mobility data.
AU  - Letertre,M
AU  - Munjoma,NC
AU  - Slade,SE
AU  - Plumb,RS
AU  - Swann,J
AU  - Coen,M
AU  - Nicholson,JK
AU  - Wilson,ID
DO  - 10.1007/s10337-020-03900-4
EP  - 861
PY  - 2020///
SN  - 0009-5893
SP  - 853
TI  - Metabolic phenotyping using UPLC–MS and rapid microbore UPLC–IM–MS: determination of the effect of different dietary regimes on the urinary metabolome of the rat
T2  - Chromatographia
UR  - http://dx.doi.org/10.1007/s10337-020-03900-4
UR  - http://hdl.handle.net/10044/1/82149
VL  - 83
ER  -
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