Imperial College London
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DrJohnPinney

Central Faculty, Graduate School

Teaching Fellow- Data Science Skills Leader
 
 
 
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Contact

 

+44 (0)20 7594 8629j.pinney

 
 
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Location

 

327Sherfield BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Humphries:2009:10.1126/scisignal.2000396,
author = {Humphries, JD and Byron, A and Bass, MD and Craig, SE and Pinney, JW and Knight, D and Humphries, MJ},
doi = {10.1126/scisignal.2000396},
journal = {Sci Signal},
title = {Proteomic analysis of integrin-associated complexes identifies RCC2 as a dual regulator of Rac1 and Arf6.},
url = {http://dx.doi.org/10.1126/scisignal.2000396},
volume = {2},
year = {2009}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The binding of integrin adhesion receptors to their extracellular matrix ligands controls cell morphology, movement, survival, and differentiation in various developmental, homeostatic, and disease processes. Here, we report a methodology to isolate complexes associated with integrin adhesion receptors, which, like other receptor-associated signaling complexes, have been refractory to proteomic analysis. Quantitative, comparative analyses of the proteomes of two receptor-ligand pairs, alpha(4)beta(1)-vascular cell adhesion molecule-1 and alpha(5)beta(1)-fibronectin, defined both core and receptor-specific components. Regulator of chromosome condensation-2 (RCC2) was detected in the alpha(5)beta(1)-fibronectin signaling network at an intersection between the Rac1 and adenosine 5'-diphosphate ribosylation factor 6 (Arf6) subnetworks. RCC2 knockdown enhanced fibronectin-induced activation of both Rac1 and Arf6 and accelerated cell spreading, suggesting that RCC2 limits the signaling required for membrane protrusion and delivery. Dysregulation of Rac1 and Arf6 function by RCC2 knockdown also abolished persistent migration along fibronectin fibers, indicating a functional role for RCC2 in directional cell movement. This proteomics workflow now opens the way to further dissection and systems-level analyses of adhesion signaling.
AU  - Humphries,JD
AU  - Byron,A
AU  - Bass,MD
AU  - Craig,SE
AU  - Pinney,JW
AU  - Knight,D
AU  - Humphries,MJ
DO  - 10.1126/scisignal.2000396
PY  - 2009///
TI  - Proteomic analysis of integrin-associated complexes identifies RCC2 as a dual regulator of Rac1 and Arf6.
T2  - Sci Signal
UR  - http://dx.doi.org/10.1126/scisignal.2000396
UR  - https://www.ncbi.nlm.nih.gov/pubmed/19738201
VL  - 2
ER  -
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