Imperial College London
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DrJohnPinney

Central Faculty, Graduate School

Teaching Fellow- Data Science Skills Leader
 
 
 
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Contact

 

+44 (0)20 7594 8629j.pinney

 
 
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Location

 

327Sherfield BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Williams:2013:10.1186/1471-2164-14-436,
author = {Williams, KJ and Bryant, WA and Jenkins, VA and Barton, GR and Witney, AA and Pinney, JW and Robertson, BD},
doi = {10.1186/1471-2164-14-436},
journal = {BMC Genomics},
title = {Deciphering the response of Mycobacterium smegmatis to nitrogen stress using bipartite active modules},
url = {http://dx.doi.org/10.1186/1471-2164-14-436},
volume = {14},
year = {2013}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BackgroundThe ability to adapt to environments with fluctuating nutrient availability is vital for bacterial survival. Although essential for growth, few nitrogen metabolism genes have been identified or fully characterised in mycobacteria and nitrogen stress survival mechanisms are unknown.ResultsA global transcriptional analysis of the mycobacterial response to nitrogen stress, showed a significant change in the differential expression of 16% of the Mycobacterium smegmatis genome. Gene expression changes were mapped onto the metabolic network using Active Modules for Bipartite Networks (AMBIENT) to identify metabolic pathways showing coordinated transcriptional responses to the stress. AMBIENT revealed several key features of the metabolic response not identified by KEGG enrichment alone. Down regulated reactions were associated with the general reduction in cellular metabolism as a consequence of reduced growth rate. Up-regulated modules highlighted metabolic changes in nitrogen assimilation and scavenging, as well as reactions involved in hydrogen peroxide metabolism, carbon scavenging and energy generation.ConclusionsApplication of an Active Modules algorithm to transcriptomic data identified key metabolic reactions and pathways altered in response to nitrogen stress, which are central to survival under nitrogen limiting environments.
AU  - Williams,KJ
AU  - Bryant,WA
AU  - Jenkins,VA
AU  - Barton,GR
AU  - Witney,AA
AU  - Pinney,JW
AU  - Robertson,BD
DO  - 10.1186/1471-2164-14-436
PY  - 2013///
SN  - 1471-2164
TI  - Deciphering the response of Mycobacterium smegmatis to nitrogen stress using bipartite active modules
T2  - BMC Genomics
UR  - http://dx.doi.org/10.1186/1471-2164-14-436
UR  - http://hdl.handle.net/10044/1/24074
VL  - 14
ER  -
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