Imperial College London
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DrJesusRodriguez Manzano

Faculty of Medicine, Department of Infectious Disease

Senior Lecturer in Diagnostics for Infectious Disease
 
 
 
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Contact

 

j.rodriguez-manzano

 
 
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Location

 

Commonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Cacho-Soblechero:2020:10.1109/TBCAS.2020.2978000,
author = {Cacho-Soblechero, M and Malpartida-Cardenas, K and Cicatiello, C and Rodriguez-Manzano, J and Georgiou, P},
doi = {10.1109/TBCAS.2020.2978000},
journal = {IEEE Transactions on Biomedical Circuits and Systems},
pages = {477--489},
title = {A dual-sensing thermo-chemical ISFET array for DNA-based diagnostics.},
url = {http://dx.doi.org/10.1109/TBCAS.2020.2978000},
volume = {14},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - This paper presents a 32x32 ISFET array with in-pixel dual-sensing and programmability targeted for on-chip DNA amplification detection. The pixel architecture provides thermal and chemical sensing by encoding temperature and ion activity in a single output PWM, modulating its frequency and its duty cycle respectively. Each pixel is composed of an ISFET-based differential linear OTA and a 2-stage sawtooth oscillator. The operating point and characteristic response of the pixel can be programmed, enabling trapped charge compensation and enhancing the versatility and adaptability of the architecture. Fabricated in 0.18 μm standard CMOS process, the system demonstrates a quadratic thermal response and a highly linear pH sensitivity, with a trapped charge compensation scheme able to calibrate 99.5% of the pixels in the target range, achieving a homogeneous response across the array. Furthermore, the sensing scheme is robust against process variations and can operate under various supply conditions. Finally, the architecture suitability for on-chip DNA amplification detection is proven by performing Loop-mediated Isothermal Amplification (LAMP) of phage lambda DNA, obtaining a time-to-positive of 7.71 minutes with results comparable to commercial qPCR instruments. This architecture represents the first in-pixel dual thermo-chemical sensing in ISFET arrays for Lab-on-a-Chip diagnostics.
AU  - Cacho-Soblechero,M
AU  - Malpartida-Cardenas,K
AU  - Cicatiello,C
AU  - Rodriguez-Manzano,J
AU  - Georgiou,P
DO  - 10.1109/TBCAS.2020.2978000
EP  - 489
PY  - 2020///
SN  - 1932-4545
SP  - 477
TI  - A dual-sensing thermo-chemical ISFET array for DNA-based diagnostics.
T2  - IEEE Transactions on Biomedical Circuits and Systems
UR  - http://dx.doi.org/10.1109/TBCAS.2020.2978000
UR  - https://www.ncbi.nlm.nih.gov/pubmed/32149696
UR  - https://ieeexplore.ieee.org/document/9022959
UR  - http://hdl.handle.net/10044/1/77659
VL  - 14
ER  -
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