Imperial College London
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DrJesusRodriguez Manzano

Faculty of Medicine, Department of Infectious Disease

Senior Lecturer in Diagnostics for Infectious Disease
 
 
 
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Contact

 

j.rodriguez-manzano

 
 
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Location

 

Commonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@inproceedings{Alexandrou:2020:10.1109/ISCAS45731.2020.9180622,
author = {Alexandrou, G and Moser, N and Rodriguez-Manzano, J and Georgiou, P and Shaw, J and Coombes, C and Toumazou, C and Kalofonou, M},
doi = {10.1109/ISCAS45731.2020.9180622},
pages = {1--5},
publisher = {IEEE},
title = {Detection of breast cancer ESR1 p.E380Q mutation on an ISFET lab-on-chip platform},
url = {http://dx.doi.org/10.1109/ISCAS45731.2020.9180622},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - CPAPER
AB  - This paper presents a method for detection of ESR1 p.E380Q, a common Breast Cancer (BC) mutation, using an ISFET (Ion-Sensitive Field-Effect Transistor) based Lab-on-Chip (LoC) platform. The LoC contains an ISFET array that can detect pH changes during DNA amplification, specifically Loop-Mediated Isothermal Amplification (LAMP). Synthetic ESR1 DNA was detected in a comparison pH-LAMP assay, carried out on the LoC platform as well as a conventional qPCR instrument. Positive detection of the allele arises due to bespoke allele-specific primers that target one base-pair difference between the wild-type and mutant alleles. The LoC and qPCR demonstrate comparable results detecting the mutant allele with mutant primers in around 25 minutes. The sensing microchip technology coupled with the molecular methods of isothermal chemistries and primer design allow this platform to be tested at a Point-of-Care setting for breast cancer patients, offering mutational tracking platform of circulating tumour DNA in liquid biopsies to assist patient stratification and allow tailored treatments.
AU  - Alexandrou,G
AU  - Moser,N
AU  - Rodriguez-Manzano,J
AU  - Georgiou,P
AU  - Shaw,J
AU  - Coombes,C
AU  - Toumazou,C
AU  - Kalofonou,M
DO  - 10.1109/ISCAS45731.2020.9180622
EP  - 5
PB  - IEEE
PY  - 2020///
SN  - 0271-4302
SP  - 1
TI  - Detection of breast cancer ESR1 p.E380Q mutation on an ISFET lab-on-chip platform
UR  - http://dx.doi.org/10.1109/ISCAS45731.2020.9180622
UR  - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000696570700235&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=a2bf6146997ec60c407a63945d4e92bb
UR  - https://ieeexplore.ieee.org/document/9180622
ER  -
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