Imperial College London
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DrJesusRodriguez Manzano

Faculty of Medicine, Department of Infectious Disease

Senior Lecturer in Diagnostics for Infectious Disease
 
 
 
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Contact

 

j.rodriguez-manzano

 
 
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Location

 

Commonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Rodriguez-Manzano:2021:10.1021/acscentsci.0c01288,
author = {Rodriguez-Manzano, J and Malpartida-Cardenas, K and Moser, N and Pennisi, I and Cavuto, M and Miglietta, L and Moniri, A and Penn, R and Satta, G and Randell, P and Davies, F and Bolt, F and Barclay, W and Holmes, A and Georgiou, P},
doi = {10.1021/acscentsci.0c01288},
journal = {ACS Central Science},
pages = {307--317},
title = {Handheld point-of-care system for rapid detection of SARS-CoV-2 extracted RNA in under 20 min},
url = {http://dx.doi.org/10.1021/acscentsci.0c01288},
volume = {7},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The COVID-19 pandemic is a global health emergency characterized by the high rate of transmission and ongoing increase of cases globally. Rapid point-of-care (PoC) diagnostics to detect the causative virus, SARS-CoV-2, are urgently needed to identify and isolate patients, contain its spread and guide clinical management. In this work, we report the development of a rapid PoC diagnostic test (<20 min) based on reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) and semiconductor technology for the detection of SARS-CoV-2 from extracted RNA samples. The developed LAMP assay was tested on a real-time benchtop instrument (RT-qLAMP) showing a lower limit of detection of 10 RNA copies per reaction. It was validated against extracted RNA from 183 clinical samples including 127 positive samples (screened by the CDC RT-qPCR assay). Results showed 91% sensitivity and 100% specificity when compared to RT-qPCR and average positive detection times of 15.45 ± 4.43 min. For validating the incorporation of the RT-LAMP assay onto our PoC platform (RT-eLAMP), a subset of samples was tested (n = 52), showing average detection times of 12.68 ± 2.56 min for positive samples (n = 34), demonstrating a comparable performance to a benchtop commercial instrument. Paired with a smartphone for results visualization and geolocalization, this portable diagnostic platform with secure cloud connectivity will enable real-time case identification and epidemiological surveillance.
AU  - Rodriguez-Manzano,J
AU  - Malpartida-Cardenas,K
AU  - Moser,N
AU  - Pennisi,I
AU  - Cavuto,M
AU  - Miglietta,L
AU  - Moniri,A
AU  - Penn,R
AU  - Satta,G
AU  - Randell,P
AU  - Davies,F
AU  - Bolt,F
AU  - Barclay,W
AU  - Holmes,A
AU  - Georgiou,P
DO  - 10.1021/acscentsci.0c01288
EP  - 317
PY  - 2021///
SN  - 2374-7943
SP  - 307
TI  - Handheld point-of-care system for rapid detection of SARS-CoV-2 extracted RNA in under 20 min
T2  - ACS Central Science
UR  - http://dx.doi.org/10.1021/acscentsci.0c01288
UR  - http://hdl.handle.net/10044/1/85853
VL  - 7
ER  -
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