Imperial College London
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DrJesusRodriguez Manzano

Faculty of Medicine, Department of Infectious Disease

Senior Lecturer in Diagnostics for Infectious Disease
 
 
 
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Contact

 

j.rodriguez-manzano

 
 
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Location

 

Commonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Cavallo:2021:10.1021/acssensors.0c02605,
author = {Cavallo, FR and Mirza, KB and de, Mateo S and Nikolic, K and Rodriguez-Manzano, J and Toumazou, C},
doi = {10.1021/acssensors.0c02605},
journal = {ACS Sensors},
pages = {709--715},
title = {Aptasensor for quantification of leptin through PCR amplification of short DNA-aptamers.},
url = {http://dx.doi.org/10.1021/acssensors.0c02605},
volume = {6},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Protein quantification is traditionally performed through enzyme-linked immunosorbent assay (ELISA), which involves long preparation times. To overcome this, new approaches use aptamers as an alternative to antibodies. In this paper, we present a new approach to quantify proteins with short DNA aptamers through polymerase chain reaction (PCR) resulting in shorter protocol times with comparatively improved limits of detection. The proposed method includes a novel way to quantify both the target protein and the corresponding short DNA-aptamers simultaneously, which also allows us to fully characterize the performance of aptasensors. Human leptin is used as a target protein to validate this technique, because it is considered an important biomarker for obesity-related studies. In our experiments, we achieved the lowest limit of detection of 100 pg/mL within less than 2 h, a limit affected by the dissociation constant of the leptin aptamer, which could be improved by selecting a more specific aptamer. Because of the simple and inexpensive approach, this technique can be employed for Lab-On-Chip implementations and for rapid "on-site" quantification of proteins.
AU  - Cavallo,FR
AU  - Mirza,KB
AU  - de,Mateo S
AU  - Nikolic,K
AU  - Rodriguez-Manzano,J
AU  - Toumazou,C
DO  - 10.1021/acssensors.0c02605
EP  - 715
PY  - 2021///
SN  - 2379-3694
SP  - 709
TI  - Aptasensor for quantification of leptin through PCR amplification of short DNA-aptamers.
T2  - ACS Sensors
UR  - http://dx.doi.org/10.1021/acssensors.0c02605
UR  - https://www.ncbi.nlm.nih.gov/pubmed/33650854
UR  - https://pubs.acs.org/doi/10.1021/acssensors.0c02605
VL  - 6
ER  -
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