Imperial College London
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DrJesusRodriguez Manzano

Faculty of Medicine, Department of Infectious Disease

Senior Lecturer in Diagnostics for Infectious Disease
 
 
 
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Contact

 

j.rodriguez-manzano

 
 
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Location

 

Commonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Miglietta:2023:10.1038/s42003-023-05235-w,
author = {Miglietta, L and Chen, Y and Luo, Z and Xu, K and Ding, N and Peng, T and Moniri, A and Kreitmann, L and Cacho-Soblechero, M and Holmes, A and Georgiou, P and Rodriguez-Manzano, J},
doi = {10.1038/s42003-023-05235-w},
journal = {Communications Biology},
title = {Smart-Plexer: a breakthrough workflow for hybrid development of multiplex PCR assays.},
url = {http://dx.doi.org/10.1038/s42003-023-05235-w},
volume = {6},
year = {2023}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Developing multiplex PCR assays requires extensive experimental testing, the number of which exponentially increases by the number of multiplexed targets. Dedicated efforts must be devoted to the design of optimal multiplex assays ensuring specific and sensitive identification of multiple analytes in a single well reaction. Inspired by data-driven approaches, we reinvent the process of developing and designing multiplex assays using a hybrid, simple workflow, named Smart-Plexer, which couples empirical testing of singleplex assays and computer simulation to develop optimised multiplex combinations. The Smart-Plexer analyses kinetic inter-target distances between amplification curves to generate optimal multiplex PCR primer sets for accurate multi-pathogen identification. In this study, the Smart-Plexer method is applied and evaluated for seven respiratory infection target detection using an optimised multiplexed PCR assay. Single-channel multiplex assays, together with the recently published data-driven methodology, Amplification Curve Analysis (ACA), were demonstrated to be capable of classifying the presence of desired targets in a single test for seven common respiratory infection pathogens.
AU  - Miglietta,L
AU  - Chen,Y
AU  - Luo,Z
AU  - Xu,K
AU  - Ding,N
AU  - Peng,T
AU  - Moniri,A
AU  - Kreitmann,L
AU  - Cacho-Soblechero,M
AU  - Holmes,A
AU  - Georgiou,P
AU  - Rodriguez-Manzano,J
DO  - 10.1038/s42003-023-05235-w
PY  - 2023///
SN  - 2399-3642
TI  - Smart-Plexer: a breakthrough workflow for hybrid development of multiplex PCR assays.
T2  - Communications Biology
UR  - http://dx.doi.org/10.1038/s42003-023-05235-w
UR  - https://www.ncbi.nlm.nih.gov/pubmed/37689821
UR  - http://hdl.handle.net/10044/1/106559
VL  - 6
ER  -
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