40 results found
Biggs MB, Medlock GL, Moutinho TJ, et al., Systems-level metabolism of the Altered Schaedler Flora, a complete gut microbiota, ISME Journal, ISSN: 1751-7362
The Altered Schaedler Flora (ASF) is a model microbial community with both in vivo and in vitro relevance. Here we provide the first characterization of the ASF community in vitro, independent of a murine host. We compared the functional genetic content of the ASF to wild murine metagenomes and found that the ASF functionally represents wild microbiomes better than random consortia of similar taxonomic composition. We developed a chemically-defined medium that supported growth of seven of the eight ASF members. To elucidate the metabolic capabilities of these ASF species—including potential for interactions such as cross feeding—we performed a spent media screen and analyzed the results through dynamic growth measurements and non-targeted metabolic profiling. We found that cross-feeding is relatively rare (32 of 3 570 possible cases), but is enriched between Clostridium ASF356 and Parabacteroides ASF519. We identified many cases of emergent metabolism (856 of 3 570 possible cases). These data will inform efforts to understand ASF dynamics and spatial distribution in vivo, to design pre- and probiotics that modulate relative abundances of ASF members, and will be essential for validating computational models of ASF metabolism. Well-characterized, experimentally tractable microbial communities enable research that can translate into more effective microbiome-targeted therapies to improve human health.
Mayneris-Perxachs J, Bolick DT, Leng J, et al., Protein and zinc deficient diets modulate the murine microbiome and metabolic phenotype, American Journal of Clinical Nutrition, ISSN: 1938-3207
Background: Environmental enteropathy, linked to undernutrition and chronic infections, affects the physical and mental growth of children in developing areas worldwide. Key to understanding how these factors combine to shape developmental outcomes is first understanding the effects of nutritional deficiencies on the mammalian system, including the effect on the gut microbiota.Objective: We dissect the nutritional components of environmental enteropathy by analyzing the specific metabolic and gut microbiota changes that occur in weaned mouse models of zinc or protein deficiency as compared to well-nourished controls. Design: Using a 1H NMR spectroscopy-based metabolic profiling approach with matching 16S microbiota analyses, the metabolic consequences and specific effects on the fecal microbiota of protein and zinc deficiency were probed independently in a murine model.Results: We find considerable shifts within the intestinal microbiota 14-24d post-weaning in mice maintained on a normal diet (including increases in Proteobacteria and striking decreases in Bacterioidetes). While the zinc deficient microbiota were comparable to the age-matched well-nourished profile, the protein-restricted microbiota remained closer in composition to the weaned enterotype with retention of Bacteroidetes. Striking increases in Verrucomicrobia (predominantly Akkermansia muciniphila) were observed in both well-nourished and protein-deficient mice 14d post-weaning. We find that protein malnutrition impairs growth and has major metabolic consequences (much more than zinc deficiency) that include altered energy, polyamine and purine/pyrimidine metabolism. Consistent with major changes in the gut microbiota, reductions in microbial proteolysis and increases in microbial dietary choline processing were observed.
Rowland I, Gibson G, Heinken A, et al., Gut microbiota functions: metabolism of nutrients and other food components, European Journal of Nutrition, ISSN: 1436-6207
Bartelt LA, Bolick DT, Mayneris-Perxachs J, et al., 2017, Cross-modulation of pathogen-specific pathways enhances malnutrition during enteric co-infection with Giardia lamblia and enteroaggregative Escherichia coli, PLOS Pathogens, Vol: 13, Pages: e1006471-e1006471
Bolick DT, Mayneris-Perxachs J, Medlock GL, et al., 2017, Increased urinary trimethylamine N-oxide (TMAO) following Cryptosporidium infection and protein malnutrition independent of microbiome effects, The Journal of Infectious Diseases, ISSN: 0022-1899
Grimaldi R, Cela D, Swann JR, et al., 2017, In vitro fermentation of B-GOS: impact on faecal bacterial populations and metabolic activity in autistic and non-autistic children, FEMS Microbiology Ecology, Vol: 93, Pages: fiw233-fiw233
Li JV, Swann J, Marchesi JR, 2017, Biology of the Microbiome 2: Metabolic Role, Gastroenterology Clinics of North America, Vol: 46, Pages: 37-47, ISSN: 0889-8553
© 2016 Elsevier Inc. The human microbiome is a new frontier in biology and one that is helping to define what it is to be human. Recently, we have begun to understand that the “communication” between the host and its microbiome is via a metabolic superhighway. By interrogating and understanding the molecules involved we may start to know who the main players are, and how we can modulate them and the mechanisms of health and disease.
Massot-Cladera M, Mayneris-Perxachs J, Costabile A, et al., 2017, Association between urinary metabolic profile and the intestinal effects of cocoa in rats, British Journal of Nutrition, Vol: 117, Pages: 623-634, ISSN: 0007-1145
Swann JR, Garcia-Perez I, Braniste V, et al., 2017, Application of H-1 NMR spectroscopy to the metabolic phenotyping of rodent brain extracts: A metabonomic study of gut microbial influence on host brain metabolism, JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, Vol: 143, Pages: 141-146, ISSN: 0731-7085
Bartelt LA, Swann JR, Guerrant RL, 2016, Decoding Hidden Messages: Can Fecal Host Transcriptomics Open Pathways to Understanding Environmental Enteropathy?, Cellular and Molecular Gastroenterology and Hepatology, Vol: 2, Pages: 114-115, ISSN: 2352-345X
Bottin JH, Swann JR, Cropp E, et al., 2016, Mycoprotein reduces energy intake and postprandial insulin release without altering glucagon-like peptide-1 and peptide tyrosine-tyrosine concentrations in healthy overweight and obese adults: a randomised-controlled trial, BRITISH JOURNAL OF NUTRITION, Vol: 116, Pages: 360-374, ISSN: 0007-1145
Darzi J, Frost GS, Swann JR, et al., 2016, L-rhamnose as a source of colonic propionate inhibits insulin secretion but does not influence measures of appetite or food intake, APPETITE, Vol: 98, Pages: 142-149, ISSN: 0195-6663
Farshim P, Walton G, Chakrabarti B, et al., 2016, Maternal Weaning Modulates Emotional Behavior and Regulates the Gut-Brain Axis, SCIENTIFIC REPORTS, Vol: 6, ISSN: 2045-2322
Grimaldi R, Swann JR, Vulevic J, et al., 2016, Fermentation properties and potential prebiotic activity of Bimuno® galacto-oligosaccharide (65 % galacto-oligosaccharide content) on in vitro gut microbiota parameters, British Journal of Nutrition, Vol: 116, Pages: 480-486, ISSN: 0007-1145
Guerrant RL, Leite AM, Pinkerton R, et al., 2016, Biomarkers of Environmental Enteropathy, Inflammation, Stunting, and Impaired Growth in Children in Northeast Brazil, PLOS ONE, Vol: 11, Pages: e0158772-e0158772
Mayneris-Perxachs J, Lima AAM, Guerrant RL, et al., 2016, Urinary N-methylnicotinamide and β-aminoisobutyric acid predict catch-up growth in undernourished Brazilian children, Scientific Reports, Vol: 6
Omairi S, Matsakas A, Degens H, et al., 2016, Enhanced exercise and regenerative capacity in a mouse model that violates size constraints of oxidative muscle fibres, eLife, Vol: 5, ISSN: 2050-084X
A central tenet of skeletal muscle biology is the existence of an inverse relationship between the oxidative fibre capacity and its size. However, robustness of this relationship is unknown. We show that superimposition of Estrogen-related receptor gamma (Errγ) on the myostatin (Mtn) mouse null background (Mtn-/-/ErrγTg/+) results in hypertrophic muscle with a high oxidative capacity thus violating the inverse relationship between fibre size and oxidative capacity. We also examined the canonical view that oxidative muscle phenotype positively correlate with Satellite cell number, the resident stem cells of skeletal muscle. Surprisingly, hypertrophic fibres from Mtn-/-/ErrγTg/+ mouse showed satellite cell deficit which unexpectedly did not affect muscle regeneration. These observations 1) challenge the concept of a constraint between fibre size and oxidative capacity and 2) indicate the important role of the microcirculation in the regenerative capacity of a muscle even when satellite cell numbers are reduced.
Collins-Hooper H, Sartori R, Giallourou N, et al., 2015, Symmorphosis through Dietary Regulation: A Combinatorial Role for Proteolysis, Autophagy and Protein Synthesis in Normalising Muscle Metabolism and Function of Hypertrophic Mice after Acute Starvation (vol 10, e0120524, 2015), PLOS ONE, Vol: 10, ISSN: 1932-6203
Escalona EE, Leng J, Dona AC, et al., 2015, Dominant components of the Thoroughbred metabolome characterised by H-1-nuclear magnetic resonance spectroscopy: A metabolite atlas of common biofluids, EQUINE VETERINARY JOURNAL, Vol: 47, Pages: 721-730, ISSN: 0425-1644
Matsakas A, Prosdocimo DA, Mitchell R, et al., 2015, Investigating mechanisms underpinning the detrimental impact of a high-fat diet in the developing and adult hypermuscular myostatin null mouse, SKELETAL MUSCLE, Vol: 5, ISSN: 2044-5040
Bodinham CL, Smith L, Thomas EL, et al., 2014, Efficacy of increased resistant starch consumption in human type 2 diabetes, ENDOCRINE CONNECTIONS, Vol: 3, Pages: 75-84
Daud NM, Ismail NA, Thomas EL, et al., 2014, The Impact of Oligofructose on Stimulation of Gut Hormones, Appetite Regulation and Adiposity, OBESITY, Vol: 22, Pages: 1430-1438, ISSN: 1930-7381
Frost G, Sleeth ML, Sahuri-Arisoylu M, et al., 2014, The short-chain fatty acid acetate reduces appetite via a central homeostatic mechanism, NATURE COMMUNICATIONS, Vol: 5, ISSN: 2041-1723
Frost GS, Walton GE, Swann JR, et al., 2014, Impacts of Plant-Based Foods in Ancestral Hominin Diets on the Metabolism and Function of Gut Microbiota In Vitro, MBIO, Vol: 5, ISSN: 2150-7511
Gan XT, Ettinger G, Huang CX, et al., 2014, Probiotic Administration Attenuates Myocardial Hypertrophy and Heart Failure After Myocardial Infarction in the Rat, Circulation: Heart Failure, Vol: 7, Pages: 491-499, ISSN: 1941-3289
Kok MGM, Swann JR, Wilson ID, et al., 2014, Hydrophilic interaction chromatography–mass spectrometry for anionic metabolic profiling of urine from antibiotic-treated rats, Journal of Pharmaceutical and Biomedical Analysis, Vol: 92, Pages: 98-104, ISSN: 0731-7085
Lees H, Swann J, Poucher SM, et al., 2014, Age and Microenvironment Outweigh Genetic Influence on the Zucker Rat Microbiome, PLOS ONE, Vol: 9, ISSN: 1932-6203
Reid G, Nduti N, Sybesma W, et al., 2014, Harnessing microbiome and probiotic research in sub-Saharan Africa: recommendations from an African workshop, Microbiome, Vol: 2, Pages: 12-12, ISSN: 2049-2618
Swann JR, Claus SP, 2014, Nutrimetabonomics: nutritional applications of metabolic profiling, Science Progress, Vol: 97, Pages: 41-47, ISSN: 0036-8504
This data is extracted from the Web of Science and reproduced under a licence from Thomson Reuters. You may not copy or re-distribute this data in whole or in part without the written consent of the Science business of Thomson Reuters.