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@article{Martinez-Micaelo:2016:10.1242/dmm.025528,
author = {Martinez-Micaelo, N and Gonzalez-Abuin, N and Terra, X and Ardevol, A and Pinent, M and Petretto, E and Behmoaras, JV},
doi = {10.1242/dmm.025528},
journal = {Disease Models & Mechanisms},
pages = {1231--1239},
title = {Identification of a nutrient sensing transcriptional network in monocytes by using inbred rat models of cafeteria diet},
url = {http://dx.doi.org/10.1242/dmm.025528},
volume = {9},
year = {2016}
}

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TY  - JOUR
AB  - Obesity has reached pandemic levels worldwide. The current models of dietinducedobesity in rodents use predominantly high-fat based diets that do nottake into account the consumption of variety of highly palatable, energy densefoods that are prevalent in Western society. We and others have shown thatcafeteria diet (CAF) is a robust and reproducible model of human metabolicsyndrome with tissue inflammation in the rat. We have previously shown thatinbred rat strains such as Wistar Kyoto (WKY) and Lewis (LEW) showdifferent susceptibilities to CAF diets with distinct metabolic and morphometricprofiles. Here we show a difference in plasma MCP-1 levels and investigatethe effect of CAF diet on peripheral blood monocyte transcriptome as powerfulstress-sensing immune cells in WKY and LEW rats. We found that 75.5% ofthe differentially expressed transcripts under CAF diet were up-regulated inWKY rats and were functionally related to the activation of the immuneresponse. Using a gene co-expression network constructed from the genesdifferentially expressed between CAF diet-fed LEW and WKY rats, weidentified the Acyl-CoA synthetase short-chain family member 2 (Acss2) as ahub gene for a nutrient sensing cluster of transcripts in monocytes. Acss2genomic region is significantly enriched for previously established metabolismquantitative trait loci in the rat. Notably, monocyte expression levels of Acss2significantly correlated with plasma glucose, triglyceride, leptin and NEFAlevels as well as morphometric measurements such as body weight and thetotal fat following CAF in the rat. These results show the importance of thegenetic background in nutritional genomics and identify inbred rat strains aspotential models for CAF-induced obesity.
AU  - Martinez-Micaelo,N
AU  - Gonzalez-Abuin,N
AU  - Terra,X
AU  - Ardevol,A
AU  - Pinent,M
AU  - Petretto,E
AU  - Behmoaras,JV
DO  - 10.1242/dmm.025528
EP  - 1239
PY  - 2016///
SN  - 1754-8403
SP  - 1231
TI  - Identification of a nutrient sensing transcriptional network in monocytes by using inbred rat models of cafeteria diet
T2  - Disease Models & Mechanisms
UR  - http://dx.doi.org/10.1242/dmm.025528
UR  - http://hdl.handle.net/10044/1/34475
VL  - 9
ER  -

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