Imperial College London

DrJamesMcGinty

Faculty of Natural SciencesDepartment of Physics

Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 7719james.mcginty

 
 
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Location

 

621Blackett LaboratorySouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

91 results found

Dunsby C, McGinty J, French P, 2014, Multidimensional fluorescence imaging of biological tissue, Biomedical Photonics Handbook, Second Edition: Fundamentals, Devices, and Techniques, Pages: 531-560, ISBN: 9781420085129

© 2015 by Taylor & Francis Group, LLC. This chapter aims to review multidimensional fluorescence imaging (MDFI) technology and its application to biological tissue, with a particular emphasis on fluorescence lifetime imaging (FLIM) of biological tissue with examples from our work at Imperial College London. Fluorescence imaging is flourishing tremendously, partly driven by advances in laser and detector technology, partly by advances in labeling technologies such as genetically expressed fluorescent proteins, and partly by advances in computational analysis techniques. Increasingly, fluorescence instrumentation is developed to provide more information than just the localization or distribution of specific fluorescent molecules. Often, fluorescence signals are analyzed to provide information on the local fluorophore environment or to contrast different fluorophores in complex mixtures-as often occur in biological tissue. This trend to higher-content fluorescence imaging increasingly exploits MDFI and measurement capabilities with instrumentation that resolves fluorescence lifetime together with other spectroscopic parameters such as excitation and emission wavelength and polarization, providing image information in two or three spatial dimensions as well as with respect to elapsed time (Figure 18.1). However, caution should be exercised when acquiring such MDFI since photobleaching or experimental considerations usually impose a limited photon budget and/or a maximum image acquisition time and also present significant challenges with respect to data analysis and data management. These considerations are particularly important for real-time clinical diagnostic applications, for higher-throughput assays, and for the investigation of dynamic biological systems (Figure 18.1).

Book chapter

Xavier GDS, Bellomo EA, McGinty JA, French PM, Rutter GAet al., 2013, Animal Models of GWAS-Identified Type 2 Diabetes Genes, Journal of Diabetes Research, Vol: 2013, ISSN: 2314-6753

More than 65 loci, encoding up to 500 different genes, have been implicated by genome-wide association studies (GWAS) as conferring an increased risk of developing type 2 diabetes (T2D). Whilst mouse models have in the past been central to understanding the mechanisms through which more penetrant risk genes for T2D, for example, those responsible for neonatal or maturity-onset diabetes of the young, only a few of those identified by GWAS, notably TCF7L2 and ZnT8/SLC30A8, have to date been examined in mouse models. We discuss here the animal models available for the latter genes and provide perspectives for future, higher throughput approaches towards efficiently mining the information provided by human genetics.

Journal article

Coda S, Kelly DJ, Lagarto JL, Manning HB, Patalay R, Sparks H, Thompson AJ, Warren SC, Dudhia J, Kennedy G, Nickdel MB, Talbot CB, Yamamoto K, Neil MAA, Itoh Y, McGinty J, Stamp GW, Thillainayagam AV, Dunsby C, French PMWet al., 2013, Autofluorescence lifetime imaging and metrology for medical research and clinical diagnosis

We report the development of instrumentation to utilise autofluorescence lifetime for the study and diagnosis of disease including cancer and osteoarthritis. ©2013 The Optical Society (OSA).

Conference paper

Xavier GDS, Mondragon A, Mitchell R, Hodson DJ, Ferre J, Thorens B, McGinty JA, French PMW, Rutter GAet al., 2013, Defective glucose homeostasis in mice inactivated selectively for Tcf7l2 in the adult beta cell with an Ins1-controlled Cre, 49th Annual Meeting of the European-Association-for-the-Study-of-Diabetes (EASD), Publisher: SPRINGER, Pages: S142-S142, ISSN: 0012-186X

Conference paper

Chen L, Andrews N, Kumar S, Frankel P, McGinty J, French PMWet al., 2013, Simultaneous angular multiplexing optical projection tomography at shifted focal planes, OPTICS LETTERS, Vol: 38, Pages: 851-853, ISSN: 0146-9592

Journal article

Chen L, McGinty J, Taylor HB, Bugeon L, Lamb JR, Dallman MJ, French Pet al., 2012, Improved OPT reconstructions based on the MTF and extension to FLIM-OPT

We demonstrate the improved reconstruction of OPT datasets by incorporating the measured MTF in the reconstruction process. We also extend OPT to FLIM-OPT and demonstrate its use for imaging live zebrafish embryos displaying autofluorescence. © 2012 OSA.

Conference paper

Xavier GDS, Mondragon A, Sun G, Chen L, McGinty JA, French PM, Rutter GAet al., 2012, Abnormal glucose tolerance and insulin secretion in pancreas-specific Tcf7l2-null mice, DIABETOLOGIA, Vol: 55, Pages: 2667-2676, ISSN: 0012-186X

Journal article

Chen L, McGinty J, Taylor HB, Bugeon L, Lamb JR, Dallman MJ, French PMWet al., 2012, Incorporation of an experimentally determined MTF for spatial frequency filtering and deconvolution during optical projection tomography reconstruction, OPTICS EXPRESS, Vol: 20, Pages: 7323-7337, ISSN: 1094-4087

Journal article

Sardini A, Stuckey DW, McGinty J, Laine R, Soloviev VY, Arridge SR, Wells DJ, French PMW, Hajnal JVet al., 2012, In Vivo Investigation of Calpain Activity by Lifetime Imaging of Genetically Encoded FRET Sensors, BIOPHYSICAL JOURNAL, Vol: 102, Pages: 159A-159A, ISSN: 0006-3495

Journal article

Antkowiak M, Torres-Mapa ML, McGinty J, Chahine M, Bugeon L, Rose A, Finn A, Moleirinho S, Okuse K, Dallman M, French P, Harding SE, Reynolds P, Gunn-Moore F, Dholakia Ket al., 2012, Towards gene therapy based on femtosecond optical transfection, BIOPHOTONICS: PHOTONIC SOLUTIONS FOR BETTER HEALTH CARE III, Vol: 8427, ISSN: 0277-786X

Journal article

Soloviev VY, McGinty J, Stuckey DW, Laine R, Wylezinska-Arridge M, Wells DJ, Sardini A, Hajnal JV, French PMW, Arridge SRet al., 2011, Förster resonance energy transfer imaging in vivo with approximated radiative transfer equation, Applied Optics, Vol: 50, Pages: 6583-6590

We describe a new light transport model, which was applied to three-dimensional lifetime imaging of Förster resonance energy transfer in mice in vivo. The model is an approximation to the radiative transfer equation and combines light diffusion and ray optics. This approximation is well adopted to wide-field time-gated intensity-based data acquisition. Reconstructed image data are presented and compared with results obtained by using the telegraph equation approximation. The new approach provides improved recovery of absorption and scattering parameters while returning similar values for the fluorescence parameters.

Journal article

McGinty J, Stuckey DW, Soloviev VY, Laine R, Wylezinska-Arridge M, Wells DJ, Arridge SR, French PMW, Hajnal JV, Sardini Aet al., 2011, In vivo fluorescence lifetime tomography of a FRET probe expressed in mouse, Biomedical Optics Express, Vol: 2, Pages: 1907-1917, ISSN: 2156-7085

Förster resonance energy transfer (FRET) is a powerful biological tool for reading out cell signaling processes. In vivo use of FRET is challenging because of the scattering properties of bulk tissue. By combining diffuse fluorescence tomography with fluorescence lifetime imaging (FLIM), implemented using wide-field time-gated detection of fluorescence excited by ultrashort laser pulses in a tomographic imaging system and applying inverse scattering algorithms, we can reconstruct the three dimensional spatial localization of fluorescence quantum efficiency and lifetime. We demonstrate in vivo spatial mapping of FRET between genetically expressed fluorescent proteins in live mice read out using FLIM. Following transfection by electroporation, mouse hind leg muscles were imaged in vivo and the emission of free donor (eGFP) in the presence of free acceptor (mCherry) could be clearly distinguished from the fluorescence of the donor when directly linked to the acceptor in a tandem (eGFP-mCherry) FRET construct.

Journal article

McGinty J, Taylor HB, Chen L, Bugeon L, Lamb JR, Dallman MJ, French PMWet al., 2011, In vivo fluorescence lifetime optical projection tomography, Biomedical Optics Express, Vol: 2, Pages: 1340-1350, ISSN: 2156-7085

We demonstrate the application of fluorescence lifetime optical projection tomography (FLIM-OPT) to in vivo imaging of lysC:GFP transgenic zebrafish embryos (Danio rerio). This method has been applied to unambiguously distinguish between the fluorescent protein (GFP) signal in myeloid cells from background autofluorescence based on the fluorescence lifetime. The combination of FLIM, an inherently ratiometric method, in conjunction with OPT results in a quantitative 3-D tomographic technique that could be used as a robust method for in vivo biological and pharmaceutical research, for example as a readout of Förster resonance energy transfer based interactions.

Journal article

Kumar S, Alibhai D, Margineanu A, Laine R, Kennedy G, McGinty J, Warren S, Kelly D, Alexandrov Y, Munro I, Talbot C, Stuckey DW, Kimberly C, Viellerobe B, Lacombe F, Lam E-F, Taylor H, Dallman MJ, Stamp G, Murray EJ, Stuhmeier F, Sardini A, Katan M, Elson DS, Neil MAA, Dunsby C, French PMWet al., 2011, FLIM FRET Technology for Drug Discovery: Automated Multiwell-Plate High-Content Analysis, Multiplexed Readouts and Application in Situ, ChemPhysChem, Vol: 12, Pages: 609-626

A fluorescence lifetime imaging (FLIM) technology platform intendedto read out changes in Fçrster resonance energy transfer(FRET) efficiency is presented for the study of protein interactionsacross the drug-discovery pipeline. FLIM provides arobust, inherently ratiometric imaging modality for drug discoverythat could allow the same sensor constructs to betranslated from automated cell-based assays through smalltransparent organisms such as zebrafish to mammals. To thisend, an automated FLIM multiwell-plate reader is described forhigh content analysis of fixed and live cells, tomographic FLIMin zebrafish and FLIM FRET of live cells via confocal endomicroscopy.For cell-based assays, an exemplar application readingout protein aggregation using FLIM FRET is presented, andthe potential for multiple simultaneous FLIM (FRET) readoutsin microscopy is illustrated.

Journal article

Margineanu A, Laine R, Kumar S, Talbot C, Warren S, Kimberley C, McGinty J, Kennedy G, Sardini A, Dunsby C, Neil MAA, Katan M, French PMWet al., 2011, Multiplexed Time Lapse Fluorescence Lifetime Readouts in an Optically Sectioning Time-Gated Imaging Microscope, 55th Annual Meeting of the Biophysical-Society, Publisher: CELL PRESS, Pages: 183-183, ISSN: 0006-3495

Conference paper

Alibhai D, Kumar S, Kelly D, Warren S, Alexandrov Y, Munro I, McGinty J, Talbot C, Murray EJ, Stuhmeier F, Neil MAA, Dunsby C, French PMWet al., 2011, An automated wide-field, time-gated, optically sectioning, fluorescence lifetime imaging multiwell plate reader for high-content analysis of protein-protein interactions, Conference on Three-Dimensional and Multidimensional Microscopy - Image Acquisition and Processing XVIII, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X

Conference paper

McGinty J, Talbot C, Owen D, Grant D, Kumar S, Galletly N, Treanor B, Kennedy G, Lanigan PMP, Munro I, Elson DS, Magee A, Davis D, Stamp G, Neil M, Dunsby C, French PMWet al., 2011, Fluorescence Lifetime Imaging Microscopy, Endoscopy and Tomography, Editors: Boas, Pitris, Ramanujam, ISBN: 1420090364

Book chapter

McGinty J, Stuckey D, Laine R, Tahir KB, Neil MAA, Hajnal JV, Sardini A, French PMWet al., 2010, Time-domain fluorescence lifetime optical projection tomography

We present a platform for measuring the fluorescence lifetime distribution in mesoscopic samples (~0.1-1cm) based on optical projection tomography and time-gated imaging. This is applied to optically cleared embryos expressing a calcium sensing FRET probe. © OSA / BIOMED/DH 2010.

Conference paper

McGinty J, Galletly NP, Dunsby C, Munro I, Elson DS, Requejo-Isidro J, Cohen P, Ahmad R, Forsyth A, Thillainayagam AV, Neil MAA, French PMW, Stamp GWet al., 2010, Wide-field fluorescence lifetime imaging of cancer, BIOMEDICAL OPTICS EXPRESS, Vol: 1, Pages: 627-640, ISSN: 2156-7085

Journal article

Sun G, Tarasov AI, McGinty JA, French PM, McDonald A, Leclerc I, Rutter GAet al., 2010, LKB1 deletion with the RIP2.Cre transgene modifies pancreatic beta-cell morphology and enhances insulin secretion in vivo, AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, Vol: 298, Pages: E1261-E1273, ISSN: 0193-1849

Journal article

Sun G, Tarasov AI, McGinty J, McDonald A, Xavier GDS, Gorman T, Marley A, French PM, Parker H, Gribble F, Reimann F, Prendiville O, Carzaniga R, Viollet B, Leclerc I, Rutter GAet al., 2010, Ablation of AMP-activated protein kinase alpha 1 and alpha 2 from mouse pancreatic beta cells and RIP2.Cre neurons suppresses insulin release in vivo, DIABETOLOGIA, Vol: 53, Pages: 924-936, ISSN: 0012-186X

Journal article

Soloviev VY, McGinty J, Tahir KB, Laine R, Stuckey DW, Mohan S, Hajnal JV, Sardini A, French PMW, Arridge SRet al., 2010, Tomographic imaging of fluorescence resonance energy transfer in highly scattering media, SPIE Photonics West, Publisher: SPIE, ISSN: 1605-7422

Conference paper

Soloviev VY, McGinty J, Tahir KB, Laine R, Stuckey DW, Mohan PS, Hajnal JV, Sardini A, French PMW, Arridge SRet al., 2010, Tomographic imaging of Fluorescence Resonance Energy Transfer in highly light scattering media, Conference on Biomedical Applications of Light Scattering IV, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X

Conference paper

McGinty J, Stuckey DW, Tahir KB, Laine R, Hajnal JV, Sardini A, French PMWet al., 2010, tomoFLIM - fluorescence lifetime projection tomography, Conference on Three-Dimensional and Multidimensional Microscopy - Image Acquisition and Processing XVII, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X

Conference paper

McGinty J, Soloviev VY, Tahir KB, Laine R, Stuckey DW, Hajnal JV, Sardini A, PMW F, Arridge SRet al., 2009, Three-dimensional imaging of Forster resonance energy transfer in heterogeneous turbid media by tomographic fluorescent lifetime imaging, OPT LETT, Vol: 34, Pages: 2772-2774, ISSN: 0146-9592

We report a three-dimensional time-resolved tomographic imaging technique for localizing protein-protein interaction and protein conformational changes in turbid media based on Forster resonant energy-transfer read out using fluorescence lifetime. This application of "tomoFRET" employs an inverse scattering algorithm utilizing the diffusion approximation to the radiative-transfer equation applied to a large tomographic data set of time-gated images. The approach is demonstrated by imaging a highly scattering cylindrical phantom within which are two thin wells containing cytosol preparations of HEK293 cells expressing TN-L15, a cytosolic genetically encoded calcium Forster resonant energy-transfer sensor. A 10 mM calcium chloride solution was added to one of the wells, inducing a protein conformation change upon binding to TN-L15, resulting in Forster resonant energy transfer and a corresponding decrease in the donor fluorescence lifetime. We successfully reconstruct spatially resolved maps of the resulting fluorescence lifetime distribution as well as of the quantum efficiency, absorption, and scattering coefficients. (C) 2009 Optical Society of America

Journal article

McGinty J, Requejo-Isidro J, Munro I, Talbot CB, Kellett PA, Hares JD, Dunsby C, Neil MAA, French PMWet al., 2009, Signal-to-noise characterization of time-gated intensifiers used for wide-field time-domain FLIM, JOURNAL OF PHYSICS D-APPLIED PHYSICS, Vol: 42, ISSN: 0022-3727

Journal article

Galletly N, McGinty J, Munro I, Elson DS, Requejo-Isidro J, Dunsby C, Neil MA, Thillainayagam AV, French PM, Stamp GWet al., 2009, Fluorescence lifetime imaging of liver cancer, 107th Annual Meeting of the American-Gastroenterlogical Association, Publisher: W B Saunders Co-Elsevier Inc

Conference paper

McGinty J, Dunsby C, Auksorius E, Benninger RKP, De Beule P, Elson DS, Galletly N, Grant D, Hofmann O, Kennedy G, Kumar S, Lanigan PMP, Manning H, Munro I, Önfelt B, Owen D, Requejo-Isidro J, Suhling K, Talbot CB, Soutter P, Lever MJ, deMello AJ, Stamp GS, Neil MAA, French PMWet al., 2009, Chapter 4 Multidimensional fluorescence imaging

Book

McGinty J, Dunsby C, Auksorius E, Benninger RKP, De Beule P, Elson DS, Galletly N, Hofmann O, Kennedy G, Lanigan P, Munro I, Onfelt B, Requejo-Isidro J, Suhling K, Talbot C, Soutter P, Lever M, deMello AJ, Stamp G, Neil M, French Pet al., 2009, Multidimensional fluorescence imaging, Laboratory Techniques in Biochemistry and Molecular Biology (FLIM and FRET techniques), Editors: Gadella, Publisher: Elsevier, ISBN: 9780080915128

This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips.

Book chapter

McGinty J, Tahir KB, Soloviev VY, Laine R, Sardini A, Talbot CB, Dunsby C, Munro I, Elson DS, Hajnal JV, Neil MAA, Arridge SR, French PMWet al., 2008, Time-domain fluorescence lifetime tomography

We present a platform for fluorescence lifetime tomography utilising tuneable supercontinuum excitation and wide-field time-gated technology. Applied to optical projection and diffuse fluorescence tomography, we demonstrate 3D time-resolved fluorescence reconstruction in transparent and scattering phantoms.

Conference paper

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