Imperial College London

DrJamesMcGinty

Faculty of Natural SciencesDepartment of Physics

Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 7719james.mcginty

 
 
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Location

 

621Blackett LaboratorySouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{French:2016:10.1002/jbio.201500258,
author = {French, PMW and andrews, N and dallman, M and ramel, MC and kumar, S and alexandrov, Y and kelly, D and warren, S and kerry, L and bugeon, L and Lockwood, N and Frolov, A},
doi = {10.1002/jbio.201500258},
journal = {Journal of Biophotonics},
pages = {414--424},
title = {Visualising apoptosis in live zebrafish using fluorescence lifetime imaging with optical projection tomography to map FRET biosensor activity in space and time},
url = {http://dx.doi.org/10.1002/jbio.201500258},
volume = {9},
year = {2016}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Fluorescence lifetime imaging (FLIM) combined with optical projection tomography (OPT) has the potential to map Förster resonant energy transfer (FRET) readouts in space and time in intact transparent or near transparent live organisms such as zebrafish larvae, thereby providing a means to visualise cell signalling processes in their physiological context. Here the first application of FLIM OPT to read out biological function in live transgenic zebrafish larvae using a genetically expressed FRET biosensor is reported. Apoptosis, or programmed cell death, is mapped in 3-D by imaging the activity of a FRET biosensor that is cleaved by Caspase 3, which is a key effector of apoptosis. Although apoptosis is a naturally occurring process during development, it can also be triggered in a variety of ways, including through gamma irradiation. FLIM OPT is shown here to enable apoptosis to be monitored over time, in live zebrafish larvae via changes in Caspase 3 activation following gamma irradiation at 24 hours post fertilisation. Significant apoptosis was observed at 3.5 hours post irradiation, predominantly in the head region.
AU - French,PMW
AU - andrews,N
AU - dallman,M
AU - ramel,MC
AU - kumar,S
AU - alexandrov,Y
AU - kelly,D
AU - warren,S
AU - kerry,L
AU - bugeon,L
AU - Lockwood,N
AU - Frolov,A
DO - 10.1002/jbio.201500258
EP - 424
PY - 2016///
SN - 1864-0648
SP - 414
TI - Visualising apoptosis in live zebrafish using fluorescence lifetime imaging with optical projection tomography to map FRET biosensor activity in space and time
T2 - Journal of Biophotonics
UR - http://dx.doi.org/10.1002/jbio.201500258
UR - http://hdl.handle.net/10044/1/29014
VL - 9
ER -