Imperial College London


Faculty of Natural SciencesDepartment of Physics

Senior Lecturer



+44 (0)20 7594 7719james.mcginty




621Blackett LaboratorySouth Kensington Campus






BibTex format

author = {Marcu, L and French, PMW and Elson, DS},
doi = {10.1201/b17018},
title = {Preface},
url = {},
year = {2014}

RIS format (EndNote, RefMan)

AB - © 2015 by Taylor & Francis Group, LLC. Wide-field time-gated fluorescence lifetime imaging (FLIM) essentially entails illuminating a sample with an ultrashort pulse of excitation radiation and sampling the resulting time varying fluorescence “image” following excitation by acquiring a series of gated fluorescence intensity images recorded at different relative delays with respect to the excitation pulse. This is represented schematically in Figure 8.1. In the simplest case, a map of the mean fluorescence decay times across the field of view is obtained. If the sampling of the fluorescence decay profiles is appropriately detailed, then the entire fluorescence decay profile for each image pixel can be acquired, and the resulting data set can be fitted to complex temporal decay models. For example, a double exponential decay model is frequently used to analyze data from Förster resonant energy transfer (FRET) experiments. The acquisition of time-gated fluorescence intensity images requires a 2-D detector, normally a charge-coupled device (CCD) camera, and some kind of fast “shutter” able to sample fluorescence decay profiles on subnanosecond timescales. Such a “shutter” function cannot be provided by mechanical means or yet by electronic circuitry and is typically provided by optical image intensifiers whose gain can be modulated by varying the applied voltage.
AU - Marcu,L
AU - French,PMW
AU - Elson,DS
DO - 10.1201/b17018
PY - 2014///
SN - 9781439861677
TI - Preface
UR -
ER -