Imperial College London

DrJerryHeng

Faculty of EngineeringDepartment of Chemical Engineering

Reader in Particle Technology
 
 
 
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Contact

 

+44 (0)20 7594 0784jerry.heng

 
 
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Location

 

417AACE ExtensionSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Zhang:2018:10.1021/acsami.8b15629,
author = {Zhang, B and Mei, AR and Isbell, MA and Wang, D and Wang, Y and Tan, SF and Teo, XL and Xu, L-J and Yang, Z and Heng, JYY},
doi = {10.1021/acsami.8b15629},
journal = {ACS Applied Materials & Interfaces},
pages = {44240--44246},
title = {DNA origami as seeds for promoting protein crystallization},
url = {http://dx.doi.org/10.1021/acsami.8b15629},
volume = {10},
year = {2018}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - This study reports the first experimental evidence of DNA origami as a seed resulting in the increase in probability of protein crystallization. Using DNA origami constructed from long single-stranded M13 DNA scaffolds folded with short single-stranded DNA staples, it was found that the addition of the DNA origami in concentrations of 2-6 nM to mixtures of a well-characterized protein (Catalase) solution (1.0-7.0 mg/mL) resulted in a higher proportion of mixtures with successful crystallization, up to 11x greater. The improvement to crystallization is evident particularly for mixtures with low concentrations of Catalase (< 5 mg/mL). DNA origami in different conformations: a flat rectangular sheet and a tubular hollow cylinder, were examined. Both conformations improved crystallization as compared to control experiments without M13 DNA or non-folded M13 DNA, but exhibited little difference in the extent of protein crystallization improvement. This work confirms predictions of the potential use of DNA origami to promote protein crystallization, with potential application to systems with limited protein available or difficult to crystallize.
AU - Zhang,B
AU - Mei,AR
AU - Isbell,MA
AU - Wang,D
AU - Wang,Y
AU - Tan,SF
AU - Teo,XL
AU - Xu,L-J
AU - Yang,Z
AU - Heng,JYY
DO - 10.1021/acsami.8b15629
EP - 44246
PY - 2018///
SN - 1944-8244
SP - 44240
TI - DNA origami as seeds for promoting protein crystallization
T2 - ACS Applied Materials & Interfaces
UR - http://dx.doi.org/10.1021/acsami.8b15629
UR - https://pubs.acs.org/doi/10.1021/acsami.8b15629
UR - http://hdl.handle.net/10044/1/64731
VL - 10
ER -