Imperial College London


Faculty of EngineeringDepartment of Chemical Engineering

Reader in Particle Technology



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BibTex format

author = {Yang, H and Chen, W and Peczulis, P and Heng, JYY},
doi = {10.1021/acs.cgd.8b01534},
journal = {Crystal Growth & Design},
pages = {983--991},
title = {The development and workflow of a continuous protein crystallisation process: A case of lysozyme},
url = {},
volume = {19},
year = {2019}

RIS format (EndNote, RefMan)

AB - In the present work, a workflow on the development of a continuous protein crystallisation is introduced, with lysozyme as a model protein, from micro L screening experiments, to small scale batch crystallisation experiments in a shaking crystallisation platform, and to batch and continuous crystallisation experiments in an oscillatory flow platform. The lysozyme crystallisation investigated were for a concentration range from 30 to 100 mg/mL, shaking conditions from 100 to 200 rpm in the batch shaking crystallisation platform, and oscillatory conditions with amplitude (x) from 5 to 30 mm and frequency (f) from 0.1 to 1.0 Hz in the batch oscillatory flow crystallisation platform. We propose the use of the Reynold’s number (Re) for scaling up of the process from the shaking batch to the continuous oscillatory flow platform. Additionally, it is shown that the nucleation rate increased with increase in concentration of initial lysozyme solution, or increase in shear rate, inducing smaller size of lysozyme crystals. These indicate that continuous crystallisation platforms may offer advantages to the downstream bioprocessing of proteins.
AU - Yang,H
AU - Chen,W
AU - Peczulis,P
AU - Heng,JYY
DO - 10.1021/acs.cgd.8b01534
EP - 991
PY - 2019///
SN - 1528-7483
SP - 983
TI - The development and workflow of a continuous protein crystallisation process: A case of lysozyme
T2 - Crystal Growth & Design
UR -
UR -
VL - 19
ER -