Imperial College London
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Professor Jerry Heng

Faculty of Engineering, Department of Chemical Engineering

Professor in Particle Technology
 
 
 
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Contact

 

+44 (0)20 7594 0784jerry.heng

 
 
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Location

 

208ACE ExtensionSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Yang:2019:10.1021/acs.cgd.8b01534,
author = {Yang, H and Chen, W and Peczulis, P and Heng, JYY},
doi = {10.1021/acs.cgd.8b01534},
journal = {Crystal Growth and Design},
pages = {983--991},
title = {Development and workflow of a continuous protein crystallization process: a case of lysozyme},
url = {http://dx.doi.org/10.1021/acs.cgd.8b01534},
volume = {19},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - In the present work, a workflow on the development of a continuous protein crystallisation is introduced, with lysozyme as a model protein, from micro L screening experiments, to small scale batch crystallisation experiments in a shaking crystallisation platform, and to batch and continuous crystallisation experiments in an oscillatory flow platform. The lysozyme crystallisation investigated were for a concentration range from 30 to 100 mg/mL, shaking conditions from 100 to 200 rpm in the batch shaking crystallisation platform, and oscillatory conditions with amplitude (x) from 5 to 30 mm and frequency (f) from 0.1 to 1.0 Hz in the batch oscillatory flow crystallisation platform. We propose the use of the Reynold’s number (Re) for scaling up of the process from the shaking batch to the continuous oscillatory flow platform. Additionally, it is shown that the nucleation rate increased with increase in concentration of initial lysozyme solution, or increase in shear rate, inducing smaller size of lysozyme crystals. These indicate that continuous crystallisation platforms may offer advantages to the downstream bioprocessing of proteins.
AU  - Yang,H
AU  - Chen,W
AU  - Peczulis,P
AU  - Heng,JYY
DO  - 10.1021/acs.cgd.8b01534
EP  - 991
PY  - 2019///
SN  - 1528-7483
SP  - 983
TI  - Development and workflow of a continuous protein crystallization process: a case of lysozyme
T2  - Crystal Growth and Design
UR  - http://dx.doi.org/10.1021/acs.cgd.8b01534
UR  - https://pubs.acs.org/doi/10.1021/acs.cgd.8b01534
UR  - http://hdl.handle.net/10044/1/65501
VL  - 19
ER  -
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