Imperial College London
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Professor Jerry Heng

Faculty of Engineering, Department of Chemical Engineering

Professor in Particle Technology
 
 
 
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Contact

 

+44 (0)20 7594 0784jerry.heng

 
 
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Location

 

208ACE ExtensionSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Li:2020:10.1039/d0ce00642d,
author = {Li, X and Chen, W and Yang, H and Yang, Z and Heng, JYY},
doi = {10.1039/d0ce00642d},
journal = {CrystEngComm},
pages = {4566--4572},
title = {Protein crystal occurrence domains in selective protein crystallisation for bio-separation},
url = {http://dx.doi.org/10.1039/d0ce00642d},
volume = {22},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Bio-separation is a key bottleneck in the manufacture of biopharmaceuticals. In this work, we report experimental evidence of direct selective protein crystallisation from a binary protein mixture solution. Lysozyme–thaumatin mixtures with a wide protein composition range (0–100 mg mL−1, respectively) were tested under the same crystallisation cocktail conditions using the hanging-drop vapour-diffusion (HDVD) crystallisation method. This work demonstrates the selectivity of crystallisation from a model binary protein mixture and four crystal occurrence domains were determined as the operation windows of selective crystallisation of the target protein: 1) an unsaturated region with no crystal formation, 2 & 3) target regions with only a single type of protein crystals (lysozyme crystals only or thaumatin crystals only) and 4) a mixture region which have a mixture of both types of protein. This study demonstrates that protein crystallisation is not only applicable to high-purity protein solutions and emphasizes the vital impacts of the presence of protein impurities in the process of target protein crystallisation. The study concludes that protein crystallisation is a feasible approach to separate a target protein from a complex mixture environment which can be achieved by manipulating the crystallisation operation conditions such as mixture composition, precipitant concentration, and operation time.
AU  - Li,X
AU  - Chen,W
AU  - Yang,H
AU  - Yang,Z
AU  - Heng,JYY
DO  - 10.1039/d0ce00642d
EP  - 4572
PY  - 2020///
SN  - 1466-8033
SP  - 4566
TI  - Protein crystal occurrence domains in selective protein crystallisation for bio-separation
T2  - CrystEngComm
UR  - http://dx.doi.org/10.1039/d0ce00642d
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000549313300004&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://pubs.rsc.org/en/content/articlelanding/2020/CE/D0CE00642D#!divAbstract
UR  - http://hdl.handle.net/10044/1/83021
VL  - 22
ER  -
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